The his - tagged peacl - gfp purified from the supernatants could polymerize into green fluorescent filamentous structures with diameter, length and shape being identical to that of muscle f - actins, which could be labeled by tritc - phalloidin ( a specific agent for staining actin microfilaments ), and were identified as having a 9 nm diameter by negative staining, corresponding with that of the muscle f - actins ( 7 - 10 nm ). under polymerization conditions, his - tagged peacl - gfp polymerized with kinetics similar to those of skeleton muscle actin, that is, an obvious lag nucleation period at the beginning of polymerization and an s - like typical polymerization curve could be obtained. the critical concentration is 0. 75 umol / l near to that of chicken muscle actin ( 0. 56 umol / l ) under the same condition
熒光標記結合熒光顯微觀察表明:從可溶性上清中純化
的his - taggedpeac1 - gfp聚合形成
的微
絲不僅可以直接在熒光顯微鏡下觀察,也可被微
絲的特異標記物鬼筆環肽所標記,而且其直徑、長度以及形態上與已知
的聚合肌動蛋白熒光
絲一致;電鏡負染
的結果進一步證實其直徑為9nm ,與傳統微
絲直徑相當( 7 ? 10nm ) ;聚合曲線有明顯
的停滯期,為典型
的s型聚合曲線,聚合臨界濃度為0 . 75 mol l ,這一結果與已有報道相
似。
Plugs and socket - outlets for household and similar purposes. part 2 : particular requirements for fused plugs
家用和類
似用途
的插頭和插座引出線.第2部分第1節:插塞式保險
絲的特殊要求
Resemblance to the person named by the hair, the hair is about as slim, called hair vegetable
因形
似人
的頭發而得名,纖細如發
絲,俗稱頭發菜。
Their likes : their breaths, too, sweetened with tea and jam, their bracelets tittering in the struggle. - kingstown pier, stephen said
跟他們類
似的人,她們
的呼吸也給紅茶果醬弄得甜
絲絲的,扭動時,她們腕上
的鐲子在竊笑著。
A felt resembling velvet, used in making hats
絨氈一種
似絲絨
的帖,用於制帽
She turned quickly, and went out. so the girls and their mother all walked together, a child on each side of tess, holding her hand, and looking at her meditatively from time to time, as at one who was about to do great things ; her mother just behind with the smallest ; the group forming a picture of honest beauty flanked by innocence, and backed by simple souled vanity
母女倆就這樣上路一起走著,苔
絲的兩邊各有一個孩子牽著她
的手,心裏
似乎想著什麼,不時地把苔
絲看上一眼,就像在看一個正要去干一番大事業
的人一樣她母親同最小
的一個孩子走在後面這一群人構成了一幅圖畫,中間走著誠實
的美麗,兩邊伴隨著無邪
的天真,後面跟隨著頭腦簡單
的虛榮。
Believing that hatred is the manifestation of longevity after death, hashimoto begins to plan his own immortality in the afterlife. fearing that the ghost child is dangerous, the team decides to isolate the ghost but in doing so they unknowingly unleashed the mysterious source on the other end of the silk strand, a much more powerful and ferocious energy
他認為促使鬼魂存在和行動
的原因是恨。小組成員因為害怕小孩
的危險性,先發制人地將它隔絕拘禁。僅管看
似成功,這個舉動卻擾動了細
絲的另一端,釋放出另一股更恐怖凶殘
的力量。
A similar cloth of rayon
一種近
似於人造
絲的布
Tellingly, the daily telegraph carries a near - identical report, suggesting that someone at chelski may have been a little indiscreet in the company of fleet street ' s finest
同時,每日電訊報也披露一則近
似相同
的消息,表明如果有一
絲的不慎將會使艦隊街
的旗幟倒下
To her relief, he unresistingly acquiesced ; her words had apparently thrown him back into his dream, which thenceforward seemed to enter on a new phase, wherein he fancied she had risen as a spirit, and was leading him to heaven
看到克萊爾順從了她,一點兒也沒有拒絕,她才放下心來顯然他又重新回到了夢境,似乎又進入了一個新的境界,在他幻想的那個境界里,苔絲的靈魂復活了,正帶著他升入天堂。