共轉 的英文怎麼說
中文拼音 [gòngzhuǎn]
共轉
英文
corotation
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共 :
共動詞[書面語]1. (圍繞) surround2. (兩手合圍) span with the hand
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轉 :
轉構詞成分。
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To investigate the consequence of this interaction, aes - rfp fusion protein expression vector was constructed and co - transfected into nih 3t3 cells with tle1 - gfp fusion protein expression vector. confocal microscopy observation showed that aes could interact with tle1 at the cytomembrane region. moreover, this interaction inhibited the concentration of tle1 into nucleus
在構建了紅色熒光蛋白aes表達載體后,將其與tle綠色熒尤蛋白載體
共轉染細胞,
共聚焦顯微鏡觀察發現這兩種分子在胞漿中有
共存現象,而且aes的表達可抑制tlei向胞核內的聚積。
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The newly molted 5th instar larva of silkworm bombyx mori was infected with the recombinant virus
另外,通過
共轉染和篩選純化獲得了攜帶appa基因的重組家蠶桿狀病毒。
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The effectiveness of the nuclear localization signal selected was clearly tested in initial experiments. based on this, the co - transfection systems used in the study was established. we first observed the multinucleate cells and chromatin bridges in cultured hela cells when the cells are marked with gfp and hochest33342, after co - transfection with the gfp expression plasmids and rnai plasmids rhe or rhc
當分別
共轉染附加kozac序列和核定位信號的gfp與人集縮素smc亞基hcap一e特異的rnai質粒rhe和人集縮素smc亞基hca屍一c的f { nai質粒rhc時,都觀察到gfp和hochest33342標記的
轉染后hela細胞表現多核和染色質橋現象。
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We also conducted the co - transfection with the rnai plasmids rhe and rhc and the selective marker gfp expressing plasmids simultaneously. there are three kinds of combination probability that could be observed by gfp marker
在實驗中同時
共轉染人集縮素smc亞基hcap一e和hcap一c特異的rnai質粒rhe和rhc和pcdna3 . 1 + kg到hela細胞。
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They are combination of co - transfection with rhe and gfp expressing plasmids, co - transfection with rhc and gfp expressing plasmids and co - transfection with plasmids rhe and rhc and gfp expressing plasmids at the same time. whichever the combination may be, we always observed the phenomena of chromatin bridge in co - transfected cells. these results indicate that the phenomena of chromatin bridge should be the characteristic feature of deficiency in the human smc subunits
這時,存在三種可能的情況,即
共轉染rhe和pedna3 . 1 * kc 、
共轉染rhc和pedna3 . 1 + kg 、
共轉染rhe和rhc和pc洲a3 . 1 + kg ,但都觀察到明顯的染色質橋現象,進一步證明這種表型是人集縮素中smc亞基的缺陷的特徵表型。
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It is applicable to coinject two or three genes whose expression products have no antagonistic action. 4. coinjection is a simple gene transfer method which saves time and money
非桔抗基因(
轉移基因的表達產物互不抑制)的
共轉移(顯微注射法)是一種既經濟、又有效的方法。
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In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv
本研究採用脂質體
轉染方法,將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的
轉移載體質粒psy681vp3lacz與fpv - 017
共轉染雞胚成纖維細胞,經6輪蝕斑克隆、篩選、表達, pcr鑒定和dot - elisa檢測,證明該重組病毒已構建成功,並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。
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According to above consideration, experiments was carried out as below : first, targeted gene - human serum albumin ( hsa ) gene was obtained via pcr technology. secondly, the hsa gene was liganded with a plasmidprla22 whose two arms carry dna sequences necessary for crossing with the human a - lactalbumin yac to form an integration vector prla - hsa, then the integration vector plasmid was co - transformated into the right yac - bearing yeast with another plasmid plrh33 which carrys a selective gene
因此,本試驗首先擴增出整合在酵母基因組里的人血清白蛋白( hsa )基因作為目的基因,並將人血清白蛋白基因插入到一個含有人-乳白蛋白yac同源序列的重組型質粒載體,以構建整合型載體,再與另一個帶篩選基因的質粒
共轉化入含人-乳白蛋白yac的酵母細胞體內。
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When cotransforming pgbd - nifa and ad fusion genomic library into saccharomyces cerevisiae pj69 - 4a, 109 candidates interacting with nifa had been selected by testing for the expression of the his3, ade2 and lacz reporter genes
誘餌質粒和文庫質粒
共轉化釀酒酵母( saccharomycescerevisiae ) pj69 - 4a ,通過檢測報告基因his3 、 ade2及lacz的表達進行篩選,初篩得到109個陽性酵母菌落。
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Having considered the particularity in converted - wave processing we pay much attention to main links such as the generation of ccp trace gather, the azimuth angle rotation of horizontal component etc., and better converted - wave profiles have been obtained
考慮到
轉換波處理的特殊性,在處理時特別注意了
共轉換點( ccp )道集的生成、水平分量方位角旋
轉等主要環節,從而得到了較好的
轉換波剖面。
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The pbacph - egf plasmid dna was used to co - transfect bmn cell with the modified bombyx mori baculovirus bm - bacpak dna, which was first linearized by aocl. after two rounds of plaque isolation, the recombinant virus ( named bmbacph - egf ) was further verified with pcr and dot hybridization. the recombinant bmbacph - egf virus was used to infect bmn culture cells at a moi of 10
重組
轉移載體dna與經bsu36i酶切線性化的修飾型病毒bm - bacpakdna
共轉染家蠶bmn細胞,兩輪空斑篩選和純化,獲得重組病毒rbmbacph - egf ,經pcr擴增、 dna點雜交等方法鑒定證實重組病毒中已正確插入ph - egf融合基因。
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On the other hand, our previous studies have shown that the nr2b construct with a gfp tag in its n - terminus cotransfected with nr1 subunit can reach the cell surface and be detected by surface staining with anti - gfp antibody in live cells. the nr2b construct will be retained in the endoplasmic reticulum ( er ) when expressed alone in heterologous cells
我們以往的研究表明,用綠熒光蛋白( gf內在信號膚下游n末端標記nrzb亞單位,與nri
共轉染時能形成與野生型相似的nmda受體通道,且可以通過抗gfp抗體標記活細胞膜表面表達的nmda受體復合物。
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Finally, a tranfer vector named as pltk - ha was constructed based on pltk - uni with insertion of ha gene of h3 subtype srv. the pltk - ha and prv bartha - k61 genomic dna were co - transfected into 50 - 70 % confluent vero cells in 6 - cm dishes. based on the expression of lacz gene, recombinant prv were selected and purified by blue - colored virus plaque
利用脂質體介導的方法,將pltk - ha與prvbartha - k61基因組
共轉染于亞單層vero細胞,依據報告基因lacz在細胞中的表達,篩選藍色蝕斑的重組病毒,經數次蝕斑純化后, pcr鑒定、 western - blot分析。
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In y2h, rapgap from c. elegans was used as bait to screen c. elegans cdna library. after - lth screen, 63 colonies were checked
在酵母雙雜交系統中,將ppc97 rpg 」與ppc86七dna文庫
共轉化入酵母菌y190細胞,經過一lth篩選后,有63個菌落擬為陽性菌落。
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The research showed that their interactions in yeast two - hybrid system are still steady when the vector was exchanged, in contrast, the interaction was disappear when the reading frame of each positive had been changed. the four positives were subcloned into suicide plasmid so that gene mutant strains could be constructed via conjugation and homologous recombination
為了進一步驗證這些克隆的編碼產物與nifa蛋白的相互作用,將它們與nifa基因互換載體后
共轉化酵母,仍然可以激活三個報告基因的表達,而陽性克隆移碼表達的重組質粒和pgbd - nifa的
共轉化物則不能在選擇性平板上生長。
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Much of the " reform " imperative pursued by australia governments has shifted the cost burden from the public to private purses
澳大利亞政府追尋的大量「改革」的必要性已經把費用負擔從公
共轉到私人的腰包。
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After obvious cytopathogenic effects developed, virus - contained supematants were harvested, and the progeny viruses were screened for lacz - expressing viruses by a plaque assay using x - gal. single blue plaques were picked, and a recombinant prv stably expressing lacz gene ( designated as rprv - lacz ) was obtained after ten cycles of plague purification and pcr identification. the results showed that the lacz gene expression cassette was stably expressed in the recombinant rprv - lacz derived from bartha - k61 strain
該載體與具有高度感染性的bartha - k61株基因組dna通過脂質體加plus法
共轉染vero細胞,採用甲基纖維素固定病變, x - gal染色,經過10代藍斑純化獲得了一株穩定表達lacz基因的ge tk基因缺失突變株,命名為rprv - lacz 。
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Network channel terminating equipment for public switched digital service
公
共轉換數字服務的網路通道終接設備
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After multiple, plasmid ploxifn and pbs185 dna were co - transfected into the green fluorescent cell clones, then filtrated the non - fluorescent cell clones. two non - fluorescent cell clones, the green fluorescent cell clones and the cells which were co - transfected by the plasmid ploxifn and pbsiss dna were checked up by pcr
首先將質粒ploxgfpdna電
轉染牛胎兒成纖維細胞,並用g418篩選,篩選出綠色熒光細胞克隆,增殖培養之後,將質粒ploxifn和pbs185dna
共轉染熒光細胞克隆,篩選出不發光的克隆。
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It is chloride - dependent and all the abnormalities can be corrected by thiazide diuretics which is the specific antagonist of the na - cl cotransporter ( ncct ). all these indicate that the disease should be relative to the ion transportation
它以高血壓,高血鉀和代謝性酸中毒為主要臨床表現,它是氯依賴性的,臨床異常能夠被鈉-氯共轉子ncct的特異性拮抗劑? ?雙氫克尿噻糾正,說明此疾病的發生和離子轉運相關。