凍融裂解液 的英文怎麼說
中文拼音 [dòngrónglièjiěyè]
凍融裂解液
英文
frozen-thaw lysate-
Western blot analysis we treated 200 fertilized eggs in a series of steps including lysing them in l0ul of lysis buffer ( 50mm tris - hcl ph 7. 5, 250mm nacl, 5mm edta, imm dtt, 0. 1 % triton, 50mm sodium orthovanadate, looug / mg pmsf and tpck, 50ug / ml tlck, 1 ug / ml leupeptin pepstatin and aprotinin ), frozing them below - 70, and then thawing them at room temperature for 10 min
2 . westem印跡將處理組及對照組小鼠一細胞g2期受精卵各200個取出，轉移到ep - pendoff管中， 5000甲m離心4分鐘，棄去上清，加入ro川粉碎緩沖液，充分振蕩混勻，在液氮中經過2一3次的凍融循環，迫使卵細胞裂解，加人等量的樣品緩沖液沸水中煮5分鐘，用於westem印跡分析。The eppendorf tube was immediately stored at - 80 c until the kinase assay was performed. the frozen eggs were frozen and thawed for three times, then added into 25l mpf buffer
Mpf及pka活性測定：將收集的鼠卵凍融3次，使細胞裂解，分別加人f反應液25山， 30t水浴反應7ruin 。
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