制備電泳 的英文怎麼說
中文拼音 [zhìbèidiànyǒng]
制備電泳
英文
preparative electrophoresis-
Our previous studies showed existence of apoplast cam in the plant cell and cam had many extracellular functions. so it supposed cam may be one of important extracellular polypeptides and trigger the intracellular signal transduction by binding the receptor. in this study, radiolabelled ligand is used to investigate the binding characteristic of cam and a. thaliana protoplasts. and chemical crosslinking is employed to explore binding proteins in the membrane. at first, ( 35 ) ~ s - cam was produced using ( 35 ) ~ s - labeled amino acid mixture in e. coli. sds - page and autoradiograph indicated high - purified, high - specific radioactivity ( 35 ) ~ s - cam was obtained. electrophoresis of ( 35 ) ~ s - cam is the same as that of unlabeled cam with ca ~ ( 2 + ) or egta ; a quatitive of protoplasts was prepared by enzymolysis
首先，用~ （ 35 ） s標記的氨基酸混合物喂養工程菌成功地制備了~ （ 35 ） s標記的擬南芥鈣調素（ ~ （ 35 ） s - cam ） ， ~ （ 35 ） s - cam純度高、放射活度高、 ca ~ （ 2 + ）與egta存在時的電泳行為與未標記cam相同，可作為一種高靈敏性的探針用於進行受體學分析實驗；用擬南芥種子誘導愈傷，通過酶解制備了大量原生質體。That is the premise of the bg / ha electrophoresis codeposition. the laws of the electrophoresis deposition of the bg and ha partic les were found by the study on each of their deposition under the different conditions. the electrophoresis codeposition of the bg and ha particles had been studied and the bg / ha graded coating, which is compact in the bottom layer and porous near the surface layer, had been prepared on the surface of the dental implant after the low temperature heat treatment ( about 740 ) and fast firing ( 50 - 80 / min, heat preservation time was 5 - 8min. )
以bg微粉和ha微粉作為塗層原料，通過研究bg和ha微粉在非水介質中的分散情況和帶電特性，選擇冰醋酸為介質，使分散在其中的bg顆粒和ha顆粒表面均帶上正電荷，為電泳共沉積提供前提條件；通過對不同條件下bg 、 ha各自電泳沉積的研究，探索出了兩者電泳沉積的規律；通過對bg和ha在冰醋酸中電泳共沉積以及后續低溫（ 740左右）快燒（ 50 ? 80 min ，保溫5 ? 8min ）熱處理的研究，在鈦合金牙根種植體基體上成功制備出了底層緻密而表層多孔的bg ha梯度塗層。In order to produce monoclonal antibodies, first, several v. dahliae isolates were grown in liquid czapeak medium, after rinsing mycelia and eliminating zoospores, the fungal tissue was homogenized with the pestle in liquid nitrogen and then transferred to test tubes and was centrifuged in tris - hcl buffer
在制備抗原的過程中，首先液體振蕩培養了若干株棉花黃萎病菌，經過沖洗除孢子、液氮研磨，用tris - hcl抽提，再離心制得菌絲蛋白提取液，可作為電泳樣品。Preparation of hydroxyapatite powder for electrophoretic deposition by chemical precipitation method
用於電泳沉積塗層的羥基磷灰石粉體沉澱法制備At the same time, alumina polymeric sols were prepared by using al ( o - sec - bu ) 3 as precusor and sec - buoh as solvent. then compact alumina films were fabricated by electrophoretic deposition
以異丁醇鋁為前驅物、異丁醇為溶劑制備了氧化鋁聚合溶膠，進而採用電泳沉積工藝制備了緻密氧化鋁膜。Based on the infrared anomalous dispersion of compact alumina film, the character of alumina sols and the processing for fabricating compact alumina film were studied by using sol - gel method, combining dip - dropping, spin - coating method and electrophoretic deposition techniques
本文基於緻密氧化鋁膜的紅外反常色散特性，結合溶膠-凝膠技術，採用浸漬提拉法、旋覆法和電泳沉積工藝，對氧化鋁溶膠的性質和緻密氧化鋁膜的制備工藝進行了研究。The general analysis of dip - dropping, spin - coating method and electrophoretic deposition techniques shows that the compaction degree of alumina film fabricated by the former two methods are higher than those prepared by the last one. besides, spin - coating method is the most efficient and fast way to raise film thickness
綜合分析提拉法、旋覆法和電泳沉積工藝，採用前兩種工藝制備的氧化鋁膜的緻密性優于末者，並且三者中旋覆法最能快捷有效地提高膜厚。A preparation method for total protein of nosema bombycis suitable for 2 - d page
一種適用於雙向電泳的家蠶微孢子蟲總蛋白的制備方法We successfully make the nanosized diamond particles calculated on the cathode and get the composed materials by electrophoretic method. comparing to the pecvd method by which people make the cnt and nanocrystal diamond grow currently on the same substrate, it ’ s more simple, economic and easy to control. the main jobs are followed : 1
實驗中採用電泳的辦法，將金剛石超微粉沉積到陰極基片上，從而制備出合成材料，此種辦法與通過pecvd方法實現基片上碳納米管與金剛石超微粉的合成材料的生長相比，更加經濟，簡單與實用，並且更加容易控制。One 66kd band appeared except 44kd main band when go isozyme above was subjected to sds - page and ce - sds, indicating this go isozyme was similar to that from spinach leaves which contained 40kd and 66kd simultaneously. whether b - mercaptoethanol was added or not when go isozyme was subjected to in sds - page and ce - sds, 40kd main band and 66kd band still appeared, indicating two subunits were not linked by covalent disulfide. amino acid analysis shew that the ratios of basic to acidic amino acid of go isozyme and its 40kd acidic subunit were 0
菜心go同工酶的sds - page和sds -毛細管電泳（ ce - sds ）顯示，該酶除了含40kd主帶外，還有很淺的66kd帶，和之前我們提出的菠菜go同工酶含40kd酸性亞基和66kd堿性亞基相似； sos - page和ce - sds電泳中，無論加入-巰基乙醇與否， go同工酶都只有40kd主帶和66kd淺帶，表明菜心go同工酶中40kd酸性亞基和66kd堿性亞基不是以共價二硫鍵相連；用制備性sds - page法獲得菜心go同工酶的40kd亞基，並和菜心go同工酶一起測定其氨基酸組成，該go同工酶及40kd亞基的堿酸性氨基酸的比例分別為0 . 66和0 . 54 ，表明40kd亞基可能是個酸性蛋白，而66kd帶則是個堿性蛋白。This review summarizes the progression in preparation and preprocessing technologies of biological specimens. it especially introduces the preprocessing technologies, including centrifugation, filtration, dielectrophoresis, immunomagnetic separation, nucleic acid extraction
摘要介紹生物標本的制備與預處理技術的進展，其中，重點介紹生物標本的預處理技術，包括離心分離、過濾、兩相電泳、免疫磁性分離及核酸抽提方法。Having noticed that in some organic solution, the surface of the nanosized diamond particles can get some negative voltage for they can absorb some ions, we can make the nanosized diamond particles calculated on the cathode by the cathodic electrophoretic method, which is important to make the cnt and the nanosized diamond particles composed materials
在合成材料的制備過程中，考慮到金剛石超微粉在一些有機溶液中因為其表面會吸附上一些帶電的離子，所以會帶上微弱的電壓。因此能通過電泳的辦法使得金剛石超微粉在陰極得到沉積，這對碳納米管與金剛石超微粉合成材料的制備有著重要的意義。This paper presents comparative systematic research of problems in the preparation of economical mould pressing bonded ndfeb permanent magnets, which include the fabricating of economical rapidly - quenching ndfeb magnetic powder and mould pressing bonded ndfeb permanent magnets ; the effect of the ndfeb magnetic powder on the magnetic properties of the magnets and the effect of the cathode electrophoretic coating on the corrosion resistance of the ndfeb magnets
為了充分發揮我國稀土資源優勢，發展我國的ndfeb稀土永磁產業，本文比較系統地研究了高性價比的模壓成型粘結ndfeb永磁體制備過程中的相關問題，包括高性價比快淬ndfeb磁粉制備、模壓成型粘結ndfeb磁體制備、 ndfeb磁粉特性對粘結磁體磁性能的影響、 ndfeb磁體陰極電泳塗層工藝及其塗層對磁體抗蝕性的影響。Once the recombinant virus has been i - dentified, we amplified the p - 1 stock to attain the large scale, high - titer viral stock in order to initiate expression studies. the recombinant angiostatin was produced from spodoptera frugiperda 9 ( sf9 ) insect cells infected by the high titer virus stock we have prepared. the time course for expression of recombinant protein was detected by sds - page and western blot, which can determine the optimal multiplicity of infection ( moi ) and the appropriate time of harvest for the protein
表達重組蛋白angiostatin ：用制備好的帶有矗s標記的融合性angiostatin基因的高滴度重組桿狀病毒貯存液感染草地貪夜蛾sfg細胞，用sds page電泳和hsternblot對感染不同時間後分泌的重組蛋白做時間表達分析，依此確定最適的感染復數（ moi ）和感染時間，以達到重組蛋白表達水平最適化，而後大規模進行重組蛋白的表達， sds page用來分析重組蛋白， westernblot用來在蛋白表達水平低的情況下檢測表達的特異重組蛋白。Based on the theory of chemical process, a novel method of electrophoyesis had been introduced and employed to synthesize expandable graphite successfully
所以，文章從化學法制備工藝的原理入手，引入電泳插層的新制備工藝，在無硫酸介入反應條件下成功地合成了可膨脹石墨製品。2. 4 preparation of yeast telomerase, the activity of telomerase was examined by the method of trap, polyacrylamide gel electrophoresis and silver staining
2 . 4酵母端粒酶的制備，端粒重復擴增法（ trap ） 、聚丙烯酞胺凝膠電泳及硝酸銀染色法檢測端粒酶活性。Status and development of preparation and surface modification methods for electrophoretic particle used in electronic ink displays
電子墨水顯示器用電泳顆粒制備和表面改性的研究進展2. 3 preparation of yeast dna, 0. 5 % agarosegel electrophoresis, eb staining for observation
3酵母dna的制備， 0 . 5瓊脂糖凝膠電泳， eb染色及紫外觀察攝影。Construction, preparation and quantity of cea dna vaccine : plasmid pgem4 - cea containing full - length of cea cdna was cleaved by ecori, 2. 4kb fragment was recovered, and ligated with ecori - cleaved plasmid pci - neo
Pci n的構建制備和定量： ecori酶切含仍a全基因的質粒pgem 。 cea ，瓊脂糖電泳回收2分享友人