噬菌體抗體 的英文怎麼說

中文拼音 [shìjūntǐkàngtǐ]
噬菌體抗體 英文
phage antibody
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 體 : 體構詞成分。
  • 抗 : Ⅰ動詞1 （抵抗; 抵擋） resist; combat; fight 2 （拒絕; 抗拒） refuse; defy 3 （對等） contend with...
  1. As well as providing a new tool to combat bacteria now, there is interest in developing bacteriophage lysins to replace antibiotics in some applications in the future

    作為對抗這些細菌的新的藥物工具，人們對將來開發噬菌體細胞溶解酶在某些條件下代替抗生素產生了濃厚的興趣。
  2. The monoclonal antibodies ( mab ) specific for the nucleoprotein of prrsv were used to select a phage random heptapeptide library

    採用噬菌體隨機7肽庫對單克隆抗體ge3識別的抗原表位進行了鑒定。
  3. Conditioned on the successful preparation of mg7 mab, this study was conducted with an attempt to develop the mg7scfv for its further application in the targeting therapy of gastric cancer

    本研究旨在利用噬菌體呈現技術制備mg _ 7單鏈抗體，為利用其進行胃癌的靶向治療研究奠定實驗基礎。
  4. The murine phage antibody library was amplified and then panned by human chorionic gonadotropin for four rounds. the last round enriched phage clones were used to reinfect

    由鼠源噬菌體抗體庫淘選到展示有人絨毛膜促性腺激素單鏈抗體的噬菌體克隆。
  5. Hau3r gene of s. lividans 1326 is directly related to its resistance to actinophage hau3

    野生型變鉛青鏈黴菌1326中的hau3 ~ r基因與1326對噬菌體hau3的抗性直接相關。
  6. When phz2057 was introduced into s. lividans zx1, it could confer partial resistance to actinophage hau3 on s. lividans zx1

    將phz2057轉入變鉛青鏈黴菌zx1 ，能賦予變鉛青鏈黴菌zx1對噬菌體hau3的部分抗性。
  7. Detection of antigen - binding affinity of mg7 recombinant phage antibody elisa was repeated to confirm the antigen - binding affinity of positive clones screened out in the former procedure ; these positive phages were examined by restriction analysis ( ecor i and hind iii ) ; competitive elisa was performed to test the inhibitory ratio of these positive clones to the binding affinity of mg7mab and its relevant antigen, the positive dones possessing apparent inhibitory effect were singled out for later use

    X陽性克到駛知烘扳原kbbjg ）濁對陽性克隆進行限制隴臥賜析（ uwi和hedlll ）鑒定三用競爭elisatoljmg7重組噬菌體抗體性克隆對mg7單扶與其相應抗原結合忙的喇率，從中j ） ed出對mg7單抗與期眩抗原結合有抑余j效應的克隆用於進一涉研究。
  8. Culture of mg7 hybridoma cells and detection of antigen - binding affinity of mg7 mab by elisa 2. construction and identification of mg7 recombinant phage antibody library mrna was isolated from cultured mg7 hybridoma cells and converted into cdna ; the variable fragments of heavy and light chain were separately amplified and assembled into scfvs with a specially constructed dna linker by pcr. the scfvs dma was ligated into the phagmid vector pcantabse and the ligated sample was transfered into competent e. co / / tg1 to generate a bacterial form of mg7recombinant phage antibody library ; volume and recombinant ratio of the library were evaluated by means of bacterial colony counts and restriction analysis ( ecor i and hind iii )

    Mg _ 7重組噬菌體抗體庫的構建及鑒定從培養的mg _ 7雜交瘤細胞中提取並分離mrna ，反轉錄成cdna ；利用pcr分別擴增mg _ 7單抗的重鏈及輕鏈可變區基因，並通過? dna連接子將二者連接起來形成mg _ 7單鏈抗體基因；將mg _ 7單鏈抗體基因插入pcantab5e ；將連接產物轉化感受態tg1大腸桿菌，制備細菌形式的mg _ 7重組噬菌體抗體庫；通過菌落計數和限制性酶切分析（ ecor和hind ）評估mg _ 7重組噬菌體抗體庫的容量和重組率。
  9. Phage display describes a selection technique in which a peptide or protein is expressed as a fussion with a coat protein of bacteriophage, resulting in display of the fused protein on the surface of the virion, while the dna encoding the fussion resides within the virion

    自1985年gpsmith首次提出噬菌體展示技術以來，隨著生物技術的發展，噬菌體隨機肽庫已成為研究分子間相互作用的有力工具，特別是在抗原表位研究方面。
  10. In addition, no back mutation to obtain a maximal complexity ( i. e. - resistant and sensitive species coexistence ) or original ecology ( i. e. original - lysogen ) for the evolution occurs

    而且，逆反應變異回原有菌相而成對噬菌體敏感及阻抗菌相共存之最大分歧度或回歸至起始單一對噬茵體敏感菌相併未曾演化發生。
  11. In this experiment, to screen the epitope of e2 protein of classical swine fever virus ( csfv ) defined by the monoclonal antibody ( mcab ) all, which had been prepared in previous experiment, the mcab all was raised in mice and followed by purification, the concentration of protein was assayed by using the bca protein assay kit

    本室利用e2基因疫苗制備了多株單抗，為e2的抗原表位研究提供了條件，我們以噬菌體隨機12肽庫分析鑒定了豬瘟病毒e2蛋白的抗原表位，為深入研究豬瘟病毒的抗原結構、制備更有效的診斷試劑和疫苗提供更多理論依據。
  12. [ methods ] two phage - epitope libraries were screened with two anti - vegf neutralizing monoclonal antibodies - jh121 and vg189, the clones were tested by dot blotting and elisa

    並通過硝酸纖維膜斑點印跡法進行陽性克隆鑒定。 elisa分析陽性噬菌體克隆與抗體的親和力。
  13. The library was rescued with phage m13k07 in order to display scfv on the surface of the phage and to form the recombinant phage antibody library. one of positive scfv clones, named pcsal, was selected with phage - elisa after panning and screening by bull sperm three times. scfv fragment, amplified from pcsa1, was ligated to pmd18 - t vector for sequencing analysis

    取陽性重組噬菌體抗體克隆株pcsa1 ， pcr擴增其scfv基因，篩選重組子進行序列測定，發現其序列符合小鼠抗體基因的一般特徵，並且與幾株抗磷酸膽堿的抗體重鏈和輕鏈可變區序列的同源性達80以上；推測pcsa1scfv針對的抗原是磷酸膽堿類物質。
  14. In the present study, the express library of monoclonal anti - sp18 scfv ( single chain fragment variable ) gene is constructed and selected for further study of sp18 antigen on mammalian fertilization and embryogenesis. total rna were firstly isolated from these growing hybridoma cells which secretes monoclonal anti - sp18 antibodies. after obtained using rpas system, vh and vl genes were used to assemble scfv gene fragment with a linker primer

    應用重組噬菌體抗體庫技術，從分泌小鼠抗牛精子sp18抗體的雜交瘤細胞系中分離總rna ，克隆抗體重鏈和輕鏈可變區基因，加入連接肽引物（ linkerprimer ）組裝成單鏈抗體scfv （ singlechainfragmentvariable ）基因並用rs引物進行擴增， sfi 、 not酶切，回收后與pcantab5e載體相連，轉化e . colitg1宿主菌，構建單鏈抗體文庫。
  15. Selection of anti - hcg scfv from murine phage antibody library selection of anti - hcg scfv from murine phage antibody library

    從噬菌體抗體庫中淘選人絨毛膜促性腺激素的單鏈抗體
  16. Methods : a set of oligonucleotide primers were designed and used to amplify the vh and vl gene from anti - hbsag fab antibodies screened from phage antibody library. the products were cloned into puc19 vector and their sequences were analysed. the vh and vl gene fragments were tethered by a peptide linker and a leader sequence coding region, with the leader sequence added at 5 " terminus of each gene ( l - vh - linker - vl ) and designated as l - scfv

    方法以從噬菌體抗體庫中篩選獲得的抗hbsag的fab抗體基因為模板，分別擴增出其輕、重鏈可變區（ v _ l 、 v _ h ）基因，通過重組pcr方法將輕、重鏈可變區基因用連接肽（ gly _ 4ser ） _ 3的編碼序列連接，並引入前導肽編碼序列，構建具有l - v _ h - linker - v _ l結構的單鏈抗體基因。
  17. Construction of murine phage antibody library and selection of n - peptide - binding single - chain antibodies

    小鼠噬菌體抗體庫的構建和n -肽結合單鏈抗體的篩選
  18. The patterns of sds - page indicated more than 30 % of recombinant proteins could be obtained from the extract of e. coli bl21. furthermore, library of recombinant phage antibodies was constructed from total rna isolated from spleen of the female balb / c mice immunized by bull sperm

    首先用牛精子免疫雌性四周齡balb c小鼠，從其脾臟組織分離總rna ，應用重組噬菌體抗體庫技術，構建了一個針對牛精子的噬菌體抗體文庫。
  19. Panning and enrichment ofmg7 recombinant phage antibody the transformed cells were infected with m13k07 helper phage to produce a phage form of mgy recombinant phage antibody library ; after three rounds panning of the library with the mgrbinding antigen highly expressed gastric cancer cell line kato iii, the mg7 scfv displayed phage dones were selected from the enriched recombinant phages by elisa

    3 mg _ 7重組噬菌體抗體庫的淘篩用m13ko7輔助噬菌體感染轉化菌，以挽救出噬菌體形式的抗體庫；用高表達mg _ 7抗原的胃癌細胞系kato對抗體庫進行三輪淘篩；用elisa篩檢呈現有mg _ 7scfv的噬菌體單克隆。
  20. Independent clones was constructed and affinity panning of anti - n - peptide antibody fragments was carried out

    的小鼠噬菌體抗體庫，並從中淘選出對n -肽有結合活性的單鏈抗體。
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