培可黴素 的英文怎麼說
中文拼音 [péikěméisù]
培可黴素
英文
bicozamycin-
It is an important that bacteria contaminated vaccine in the biologicals production. we collected 703 samples of cell culture, virus cultivation and harvest which were contaminated by bacteria during poliovaccine production within two years. we checked these samples by bacteriological method and antibiotics sensitivity tests were done. it shows that 1 ) the main contaminated bacteria come from staphylococci, bacilli and streptococci of environment in the poliovaccine production. 2 ） it is effect that antibiotics to contaminated bacteria are doxycycline, albiotic, prescription 2, cefotaxime na salt, gentamycin, neomycin, aureomycin and erythromycin
在疫苗生產實踐中，細菌污染是影響疫苗質量和產量的關鍵性因素，筆者通過了兩年左右的時間，選取正常生產中零星細菌污染的細胞培養瓶、病毒培養瓶及收毒污染樣品等共703份，進行細菌學檢查，並對造成污染的主要細菌種類進行了各種抗菌藥物的耐藥性實驗，結果表明：我所脊灰疫苗生產中主要的污染威脅來自環境中的葡萄球菌，潛在威脅是桿菌和鏈球菌；強力黴素、林可黴素、配方2 、噻孢黴素鈉鹽、慶大黴素、新黴素、金黴素和紅黴素等抗生素對目前引起污染優勢細菌-葡萄球菌有明顯的抑菌效果，可作為疫苗生產后備抗菌手段參考The results of carp ' s growth performance indicated : the mixed nutrient solution of psb could improve the increase of carp ' s weight than other three groups ( carp ' s diet of juxing, flavomycin, " yixubao " ), they are 9. 5 %, 9. 6 %, 15. 4 % respectively. and it could decrease the feed consumed / gain of carps than others, they are 2. 6 %, 6. 3 %, 4. 8 % respectively. all those showed that the mixed nutrient solution of psb could enhance the growth performance of carp
光合細菌復合培養物作為飼料添加劑應用於鯉魚生產試驗，在飼喂35天後試驗組總增重分別比某公司魚飼料組、添加黃黴素組（ 3ppm ） 、 「益畜寶」組提高9 . 5 、 9 . 6 、 15 . 4 ，料肉比降低了2 . 6 、 6 . 3 、 4 . 8 ，說明光合細菌可以提高鯉魚的生產性能。First, after investigation of two original strains " biological characteristics, we studied the main influence factors on protoplasts formation and regeneration in s. mycarofaciens and s. erythreus, and determined the best protoplasts formation and regeneration conditions of two original strains. the former shake - cultured in s " medium at 28 ?, 220r. min ~ ( - 1 ) for 24h, lysised by 3mg / ml lysozyme, keeping warm at 32 ? for 50 ~ 60min, regenerated on r _ ( 5 " ) medium, 28 ? for 5 ~ 6d. the latter used two - step culture, then used img / ml lysozyme keeping warm at 37 ? for ih ; the protoplasts were plated on r5 " regeneration medium at 28 ? for 5d
首先在對兩親株的生物學特性進行了鑒定后，考察了影響兩親株原生質體形成和再生的主要因素，確定了生米卡鏈黴菌和紅黴素鏈黴菌原生質體形成及再生的最佳條件：前者用s培養基，在28 、 220r . min ~ （ - 1 ）培養24h后，用3mg ml的溶菌酶在32恆溫酶解50 60min ，得到的原生質體在乾燥的r5培養基上28倒置培養5 6天，可得到再生率在20左右的再生菌落；後者採用二級菌絲培養，用1mg ml的溶菌酶在37恆溫酶解1h左右，得到的原生質體也在乾燥的r5培養基上28倒置培養5天，即可得到再生率在20左右的再生菌。( 5 ) when pgcs were cultured on different feeder layers, the better results were got if pgcs were cultured on sto and mef1 feeder layers, when pgcs were cultured on thawed sto feeders, pef, inactivated pef feeders, 0. 1 % gelatin, we can not get typical eg colonies
（ 5 ）用不同飼養層培養pgc ，實驗結果證明pgc在新鮮製作的sto和mef ~ 1飼養層上，可以得到傳3代以上的eg集落； pgc在鋪有解凍的sto飼養層細胞、 pef或絲裂黴素c處理過的pef 、塗有0 . 1明膠的4孔板上，一直沒有得到典型的eg集落。Our experiment indicates : ( 1 ) the optimal concentration of kanamycin for screening torenia fournier regenerated buds was 400 mg / l. the ideal transformation was obtained in the following conditions : the leaf discs were dipped in agrobacterium suspension that od600 was 0. 1 for 10 ~ 20 min ; subsequently cocultivated on the ms solid coculture medium containing 20umol / l acetosyringon for 7 to 8 d at 23, and the induction ratio of regenerated buds was 27. 2 %
研究結果表明： （ 1 ）篩選藍豬耳轉化芽的最適卡那黴素濃度為400mg l 。 od _ （ 600 ）為0 . 1的菌液濃度菌液浸染葉盤10 20min ，固體共培養基（含20 mol l乙酰丁香酮）上23共培養7 8d可獲得理想的轉化效率，轉化芽誘導率為27 . 2 ； 16h光照， 8h黑暗是較理想的共培養光周期；莖段是較好的轉化受體。The effect of some factors, including the appropriate materials for isolating protoplasts, the concentrations of enzyme, period of digestion and temperatures, and osmotic pressure stabilizers on the isolation and regeneration of protoplasts in penicillium digitatum were studied. the results demonstrated that the purified protoplasts could regenerate through double layers of czapek medium containing 0. 7mol / l nacl. the regeneration rate could reach 24. 9 %
通過對制備材料、酶液濃度、酶解時間、酶解溫度、滲透壓穩定劑的種類和濃度等因素的實驗研究，得到了一套制備指狀青黴（ penicilliumdigitatum ）原生質體的有效方法，並在雙層培養基上初步實現了原生質體的再生，再生率可達24 . 9 。分享友人