報告基因 的英文怎麼說

中文拼音 [bàogàojīyīn]
報告基因 英文
reporter gene
  • 報 : Ⅰ動詞1 （告知; 報告） report; declare; announce 2 （回答） reply; respond; reciprocate 3 （答謝）...
  • 告 : 告動詞（由上至下告知） officially announce
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 報告 : 1. （告訴） report; make known; inform 2. （講演; 書面申請或總結） report; speech; talk; lecture; advisory
  1. First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected

    目的：構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后，利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素，初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。
  2. Comprehensive cellular responses was found in human amnion fl cells following exposure to low concentration of mnng, such as the lowering of dna replication fidelity resulted from alteration of dna polymerase profile ; activation of a lot of transcription factors, such as api, creb, nf - kb etc ; clustering of egfr ( epidermal growth factor receptor ) and tnfr ( tumor necrosis factor receptor ) and activation of camp - pka - creb and jnk / sapk signal pathways

    我們發現，低劑量mnng處理后的人羊膜fl細胞有廣泛的細胞反應，並有多個信號轉導通路的激活和基因表達的改變。例如dna復制保真度下降， dna聚合酶譜發生改變，應用報告基因技術和底物磷酸化檢出技術證明細胞一系列轉錄因子如ap1 、 creb 、 nf b等被激活，細胞表面受體如表皮生長因子受體、腫瘤壞死因子受體發生聚簇，細胞信號轉導通路camp - pka - creb和jnk sapk被激活。
  3. A genetic transformation model for the brown seaweed undaria pinnatifida has been primarily set up by using micro - particle bombardment as the method, female or male gametophytes as the gene recipients, hybridization as the regeneration route and chloramphenicol, hygromycin or basta as selective reagents

    本文從轉化受體、轉化方法、報告基因、再生途徑、篩選方法等方面對裙帶菜的遺傳轉化進行了研究。首先，分離並建立了裙帶菜雌雄配子體的無性繁殖系，進行了裙帶菜的不同再生途徑的研究。
  4. Development of a functional reporter gene assay for the identification of glucagon - like peptide - 1 receptor agonist

    1受體激動劑功能性報告基因分析系統的構建
  5. To test the p7 promoter activity, a series of constructs were obtained by cloning the different dna fragments into the luciferase gene reporter vector pgl3 - b. when the constructs were transiently transfected into thp - 1 cells, luciferase activity assay showed that the core region of p7 promoter located at - - 165 bp

    第一部分的工作首先通過對人acat - 1基因p7啟動子序列進行5 '和3 ' -端的缺失的詳細分析，結果顯示最大報告基因轉錄表達活性位於- 612 - 165bp區段。
  6. In the study of floral - dip method, flowering inflorescences of oilseed rape growing in field are immersed in floral - dip solution resuspended with cultured agrobacterium tumefaciens lba4404 or eha105, resided binary plasmid pbi121

    在油菜花序浸泡法（ noraldip ）轉基因的研究中，用含雙元載體及報告基因的農桿菌在生產大田直接浸染正在開放的油菜花朵。
  7. The expression efficiency difference between ped5 and pcdhfrl, a vector utilizing cmv enhancer / promoter ( pcmv - ie ) for foreign protein production, was analyzed using human interferon - p ( ifn - ) gene and human secreted alkaline phosphatase ( seap ) gene as reporters. when analyzed in transient expression, ped5 showed a little more protein produciton than pcdhfrl. however, in continuous expression, when serum concentration was lessened to slow down cell proliferation, ped5 expressed 3. 1 times more reporter proteins than pcdhfrl, which implied that pef - io was less affected by cell cycle status in contrast to pcmv - ie, making ped5 a good expression vector for foreign protein production

    應用人-干擾素（ ifn - ）和人分泌型堿性磷酸酶（ seap ）基因作為報告基因，對含有巨細胞病毒即早期啟動子（ p _ （ cmv - ie ） ）的表達載體pcdhfr1和ped5表達外源蛋白的能力進行了比較，發現對于瞬時表達， ped5略好於pcdhfr1 ；在穩定表達中，通過降低血清濃度，使細胞增殖緩慢，這時ped5表達外源蛋白的能力較pcdhfr1高3 . 1倍。
  8. To follow the differentiation of lepcs in vivo, lepcs were tagged with reported gene egfp, dsred and lac z by retroviral methods

    採用逆轉錄病毒介導的基因轉染手段，將lepcs標記lacz 、 egf戶和osreo作為報告基因。
  9. In this study, the recombinant fowl - poxvirus was transfected into expressing the vp3 gene of isolated gpv h1 strain into the cef cells with fpv - 017 by liposome, which have the lacz reporter gene, earlier / latter promoters lp2ep2 of fpv, promoters p7. 5 and p7. 1 of vaccinia virus, replication unnecessary region of fpv - 017. following 6 cycles screenings, clonings, purification of blue plaques, detection of pcr and dot - elisa, which verified the genetic stable vp3 - fowlpox virus recombinant constructed successfully. this study provided the theoretical and practical foundation for development of gpv recombinant fowl - poxvirus genetic engineering vaccine, as well as provided substance preparatory for prevention the high mortality gpv

    本研究採用脂質體轉染方法，將含有完整gpvh1分離株vp3基因、報告基因lacz 、禽痘病毒早晚期啟動子lp2ep2 、痘苗病毒啟動子p7 . 5 、 p11和fpv - 017復制非必須區的轉移載體質粒psy681vp3lacz與fpv - 017共轉染雞胚成纖維細胞，經6輪蝕斑克隆、篩選、表達， pcr鑒定和dot - elisa檢測，證明該重組病毒已構建成功，並獲得了遺傳性狀穩定的鵝細小病毒vp3基因的重組禽痘病毒。
  10. Improvement of genetic transformation system by using gfp as reporter gene on pepper

    應用綠色熒光蛋白報告基因優化辣椒的遺傳轉化體系
  11. Vp3 gene of hl isolate of goose parvovirus derived from recombinant piasmid pproex htb - vp3 was subcloned into ecorl site of psy538, and the reporter gene lacz with promoter pll was cloned into smai site of recombinant piasmid. both vp3 gene and lacz gene were cloned into noti site of psy681, recombinan fpv transfer vector containing vp3 gene of gpv was obtained. the result is basis of construction of recombinant fpv expressing vp3 gene of gpv and gpv genetic engineering vaccine

    本研究另從含有gpvh1分離株主要結構蛋白vp3基因的重組質粒pproexhtb - vp3中切取gpvh1株vp3基因片段，將其亞克隆于psy538的ecori位點，然後將帶有痘苗病毒啟動子p11的lacz報告基因平端克隆于上述重組子的smai位點，再用noti切下同時含有vp3基因和lacz報告基因的片段，再亞克隆于psy681的noti位點，構建出含有vp3基因的重組禽痘病毒轉移載體，為構建表達vp3基因的重組禽痘病毒從而制備gpv基因工程疫苗奠定了基礎。
  12. Cloning and identification of nf - b p50 rel homology domain and detection of its autonomous reporter gene activity

    50亞基同源結構域的克隆鑒定及自主報告基因活性的檢測
  13. The transient expression of lacz driven by crtw 306bp s ' - flanking sequence and crtz 302bp s ' - flanking sequence shows that these two sequences habour transcription regulatory sequences

    以lacz為報告基因的瞬間表達實驗結果表明，長度分別為306bp和302bp的crtw和crtz5 '上游側翼序列具有很強的啟動轉錄活性，提示兩段序列包含了啟動子的結構。
  14. When cotransforming pgbd - nifa and ad fusion genomic library into saccharomyces cerevisiae pj69 - 4a, 109 candidates interacting with nifa had been selected by testing for the expression of the his3, ade2 and lacz reporter genes

    誘餌質粒和文庫質粒共轉化釀酒酵母（ saccharomycescerevisiae ） pj69 - 4a ，通過檢測報告基因his3 、 ade2及lacz的表達進行篩選，初篩得到109個陽性酵母菌落。
  15. Smai, kpni and sali flanking bamhi could be used to analyze the cloned fragment

    為應用協心加雙重報告基因研究根瘤菌的分子生物學積累了材料。
  16. The symbiosis between mesorhizobium huakuii and astragalus sinicus is a chinese - characteristic symbiotic nitrogen fixation system, while molecular genetic study on its early symbiotic interaction is still at primary stage

    本文對新型報告基因?綠色熒光蛋白基因在華癸中生根瘤菌-紫雲英共生固氮體系早期結瘤階段分子遺傳學中的應用進行了探索性研究。
  17. Our interesting is to use the red gene as a reporter gene

    為驗證redghe能否用來作為分子生物學研究的報告基因，將克隆在clon 。
  18. 328 his + positive colonies were streaked sd / - ade / - his / - leu / - trp plates, and 58 ade + positive colonies were obtained

    經ade報告基因生長實驗進一步鑒別，篩選到58個ade +克隆。
  19. By digesting with bamhi and sacl, we confirmed that the construction of the binary expression vector was correct

    選擇帶有gus報告基因（ 1 . 87kb ）的pbii21質粒為表達載體，其篩選標記為卡那黴素。
  20. A binary plant expression vector with osg6b " driving report gene gus was constructed and transferred into tobacco via agrobacterium tumefaciens mediation

    將克隆的啟動子與報告基因gus相連，構建植物表達載體，通過農桿菌介導轉化煙草。
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