堿基對突變 的英文怎麼說
中文拼音 [jiǎnjīduìtūbiàn]
堿基對突變
英文
base-pair mutation- 堿 : 名詞1. (含氫氧根的化合物的統稱) alkali2. (碳酸鈉) soda
- 對 : Ⅰ動詞1 (回答) answer; reply 2 (對待; 對付) treat; cope with; counter 3 (朝; 向; 面對) be tr...
- 突 : Ⅰ動詞1 (猛沖) dash forward; shoot out 2 (高於周圍) protrude; bulgeⅡ副詞(突然) abruptly; sud...
- 突變 : 1 (突然急劇的變化) sudden change; change suddenly; transilience; accident; saltation; revulsion...
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Analysis of the sequence variation of cytochrome b gene indicated that there is no evidence of insertions or deletions, i. e., they are all of identical length of 1143 bp in all the sequences of cytochrome b gene. further, the sequences can be fully translated into amino acid using chicken mitochondrial codon without nonsense mutations or intervening stop codons. the 1143 bp cytochrome b alignment contained 416 variable sites, of which 306 were parsimony informative sites with the strongest variable in third codon positions and less variable in first and second codon positions
細胞色素b基因序列變異分析表明: 1 )雁形目鳥類細胞色素b基因全序列長度一致,無插入和缺失:對照雞線粒體密碼子系統全序列能全部翻譯成氨基酸序列,無無義突變,全序列內部無終止密碼子; 2 )序列比對后1143加,含416個核著酸變異位點, 306個簡約信息位點,其中處於密碼子第三位的變異最大,第一位和第二位堿基的變異相對較小。It was interested that there was an extra six nucleosides insertion between 1647 - 1652nt ( according to the genomic sequence of la sota strain ), and the sequence were cccccc in f48e9 strain, and tcccac in zj1 strain. in order to test if insertion of this six nucleosides is related with the virulence of nd, two primers were designed to amplify the same fragment of another ten ndv strains. the result of sequence comparison of 16 strains showed that the six nucleoside was absent in lentogenic strain. this suggested that the six nucleosides insertion might have relationship with the ndv virulence. compared with all known sequence of ndv. there was a special sequence ( 5 ' tctctctcctctctcctcc3 " ) in the genomic cdna of ndv f48e9 strain
通過rt - pcr方法擴增獲得了另外10個背景清楚毒株的np - p基因間隔區片段,將這些序列與f48e9 、 lasota 、 clone30 、 b1 、 zj1和v4的相應序列進行了比較,結果在參比的16個ndv毒株中在該區段中除了有多個點突變外,個別毒株有堿基插入和缺失,所有以lasota株為代表的弱毒株均無6堿基的插入,而以f48e9株為首的強毒株均有此6堿基的插入,但有一個中等毒力的毒株dp沒有6堿基的插入,不過它的基因序列和lasota的幾乎相同,對于所克隆到的基因的代表性還有待確定。Sequence analysis showed that, this fragment has a homology of 99 % to the previously reported choe from rhodococcus equi. after cloning and subcloning and three identical fragments were obtained from three independent pcr, lacking three continual bases ( ttc, encoding the pheamino acid ) compared with choe, thus it can be assumed that the new fragment, designated as choew, and choe belong to the same gene family, even it might be the natural mutation of choe
對片段進行克隆、亞克隆之後測序分析,發現與已克隆的來源於馬紅球菌的膽固醇氧化酶基因cboe有99的同源性;並且三次獨立的pcr均得到相同的片段,所克隆的基因序列與choe相比,連續缺失三個堿基( ttc ) ,即相應的氨基酸序列缺失一個氨基酸( phe ) ,因此判斷所克隆的基因片段與choe屬同一基因家族或原有基因的天然突變體,命名為choew 。( 3 ) on the basis of the deletion analysis, three substitution mutants ( ml : 6bp sequence upstream of gcc box m2 : gcc box and m3 : g box - like sequence ) by pcr were designed to isolate the essential ja - responsive element. transgenic tobacco plants containing promoter substitution constructs were generated by agrobacterium - rnqdiaied leaf transformation. loss - of - function experiment, using transient expression analysis of gus reporter genes, confirmed that gcc box act as an essential element to respond ja signaling in pdf1. 2 promoter
( 3 )在缺失突變的基礎上,通過對gccbox及其相鄰的上下游六個堿基進行取代突變,將突變啟動子與gus構建融合基因,在煙草中受heja誘導的瞬時表達結果表明, h1和m3的突變對該啟動子應答ja信號的影響很小,而m2 ( gccbox的突變)則幾乎使該啟動子應答ja信號的功能完全喪失,所以gccbox是該啟動子中應答ja信號的必需元件。We sequence the inserted gene fragment of the indentified recombinant clone. the result is : angiostatin gene orf ( open reading frame ) links with the orf in expression vector correctly. but the first base of the codon aaa coding for lys414 in plg kringle 4 domain mutates from a to g which leads lys change to glu
隨后取通過上述鑒定的重組克隆菌,對重組子插入片段測序,結果為: as基因開放讀碼框與表達載體的讀碼框正確匹配相連,但在其kringle4區相當于編碼plg的lys ~ ( 414 )密碼子aaa的第一位堿基由a突變為g ,導致相應的氨基酸殘基突變為glu 。To discriminate between drier and passenger mutations, the authors annotated the 921 single - base substitutions that they identified as being either non - synonymous ( changing an amino acid ) or synonymous ( haing no effect on the protein sequence )
為了找出驅動和旅客突變間的差別,研究人員將921種單堿基替換突變分成兩類:非同義(改變一個氨基酸)或同義(對氨基酸序列沒有影響) 。To discriminate between driver and passenger mutations, the authors annotated the 921 single - base substitutions that they identified as being either non - synonymous ( changing an amino acid ) or synonymous ( having no effect on the protein sequence )
為了找出驅動和旅客突變間的差別,研究人員將921種單堿基替換突變分成兩類:非同義(改變一個氨基酸)或同義(對氨基酸序列沒有影響) 。The sequence is 451bp in length and shows98 % homologous to isolate gi 1575050 gb u57046. 1 pnu57046 deposited in the gene bank
18pnl57046 ,對它們的核酸序列相應片段比較,僅具7個堿基突變,二者的同源性達98 ,從而確認是pnrsv 。分享友人