堿基比 的英文怎麼說
中文拼音 [jiǎnjībǐ]
堿基比
英文
base ratio-
The base pair ratio, at : gc, is constant within a species but differs between species
At : gc之間的堿基比具有種內特異性,但在不同種之間往往是不同的。The base ratio of 1 : 1 for the bases adenine and thymine and the bases cytosine and guanine was the first clue to the specificity of base pairing in the nucleic acids
在核酸中的堿基配對高度專一性的原則決定了腺嘌呤與胸腺嘧啶,或鳥嘌呤與胞嘧啶之間的比值為1 : 1 。G gene of rabies virus m, the of two main regions ( about 1000nt ), ranging from 3161nt to 4162nt and ranging from 4012nt to 4863nt of glycoprotein gene of rabies virus strain m, isolated from mouse in he nan, china were amplified by reverse transcriptase - polynerase chain reaction ( rt - pcr ) in order to complete glycoprotein gene of strain m. these regions were sequenced by the produce of pcr directly. comparison and analysis of nucleotide sequence and amino acid sequence deduced with that of other strains published was performed by computer with dnasisv 2. 5demo software
本研究對我國河南某地野鼠體內分離的狂犬病野毒株mrv基因的3161位? 4162位( 1001個堿基)和4012位? 4863位( 851個堿基)片段進行了反轉錄pcr擴增和序列測定,得到mrv的糖蛋白基因全序列,用dnasisv2 . 5demo分析軟體,與已發表的代表性毒株g基因全序列進行核苷酸和氨基酸序列的比較分析,結果表明在同一基因型中, mrv和國際標準攻毒株cvs的同源性最高( 96 . 5 ) ,和中國減毒株ctn的同源性最低( 79 . 8 ) 。The start reading framae and stop codons, base composition in protein - coding genes and the codon usage of amino acids in scolopendra multilane were compared with the three other myriapods
本研究在蛋白質編碼基因起始閱讀框和終止密碼子、蛋白質編碼區的堿基組中文摘要成、氨基酸及密碼子的利用等方面把少棘蜈蚣與另三種多足類進行了比較。The fragments were subcloned into a low copy transcription vector ( px8dt ) between the t7 rna polymerase promoter and autocatalytic hepatitis delta virus ribozyme. the result showed that genome of ndv f48e9 strain comprises 15192 nt, which was equal to zjl strain and 6 nucleosides longer than that of la sota, v4, bl and clone30
實驗結果表明: f48e9全基因組具有15192個堿基,比lasota 、 v4 、 b1和clone30的全基因組序列長6個堿基,和鵝源zj1株的長度相等。Basic silicones for industrial use. determination of ratios phenyl silicium and phenyl methyl. near infrared spectrometric method
工業用堿性硅樹脂.苯基硅和苯基甲基比率的測定.近紅外線光譜測定法Stilbene synthase genes ( l, 2 ) from vitis venefera were cloned and sequenced. the results of sequence analysis showed the total length of s2 is 1617bp, the homology between s2 and pvin ( reported ) is 99 % ; the total length of si is 1622bp, the homology between si and pvin ( reported ) is 98 %
Dna序列分析結果表明,來自雷司令的芪合酶基因全長1617bp ,與文獻報道的該基因( pvin )比較,堿基同源率為99 ,來源於粉紅玫瑰的芪合酶基因全長1622bp ,與pvin的序列比較,堿基同源率為98 。Establishing snupe for 2 y - snp loci, we understand that the suitable thermostable dn a polymerase and proportion of template and primer are the keys to reaction of snupe
通過建立2個y - snp的snupe反應,了解snupe反應的一般規律,揭示單堿基引物延伸反應的關鍵是選擇合適的dna聚合酶及合適的引物和模板的比例。Analysis of the sequence variation of cytochrome b gene indicated that there is no evidence of insertions or deletions, i. e., they are all of identical length of 1143 bp in all the sequences of cytochrome b gene. further, the sequences can be fully translated into amino acid using chicken mitochondrial codon without nonsense mutations or intervening stop codons. the 1143 bp cytochrome b alignment contained 416 variable sites, of which 306 were parsimony informative sites with the strongest variable in third codon positions and less variable in first and second codon positions
細胞色素b基因序列變異分析表明: 1 )雁形目鳥類細胞色素b基因全序列長度一致,無插入和缺失:對照雞線粒體密碼子系統全序列能全部翻譯成氨基酸序列,無無義突變,全序列內部無終止密碼子; 2 )序列比對后1143加,含416個核著酸變異位點, 306個簡約信息位點,其中處於密碼子第三位的變異最大,第一位和第二位堿基的變異相對較小。By blasting the homologous sequences in genbank databases, the sequence of grass carp gh cdna from pituitary is 98 % homologous compared with the previously cloned gh cdna of grass carp. the cgh cdna fragment was inserted into pgex - 4t - l to construct the expression plasmid. the recombinant plasmid was digested by bamh i and ecor i to identify whether the cgh cdna fragment was inserted into the plasmid, the pgcgh was transformed into e. coli bl21 competent cells
將得到的序列在genbank和embl數據庫中進行了同源比較,結果顯示:本研究克隆到的草魚gh基因與genbank中登記的x60474草魚gh基因有12個堿基的差異,編碼的氨基酸有3個氨基酸殘基的差異,同源性為98 ,影響蛋白質高級結構的保守二硫鍵為2個。However, the cloned promoter had 18 substitution at 18 sites, 22 deletions at 18 sites and 3 insertion at 3 sites between sites 0 - - - 1273 bp which was reported to control temporal - spatial specific expression, and 3 substitution at 3 sites, 6 deletions at 6 sites between - 1095 bp and - 1273 bp, the key functional sequence area, in comparing with known osg6b
但是與報道的osg6b比較,在決定時空特異性的0 - 1273bp功能區域內,有18處出現18堿基替換, 18處出現22堿基缺失, 3處出現3堿基插入;在核心功能序列區域( - 1095bp - 1273bp )內,有3處出現3堿基替換, 6處出現6堿基缺失。It was interested that there was an extra six nucleosides insertion between 1647 - 1652nt ( according to the genomic sequence of la sota strain ), and the sequence were cccccc in f48e9 strain, and tcccac in zj1 strain. in order to test if insertion of this six nucleosides is related with the virulence of nd, two primers were designed to amplify the same fragment of another ten ndv strains. the result of sequence comparison of 16 strains showed that the six nucleoside was absent in lentogenic strain. this suggested that the six nucleosides insertion might have relationship with the ndv virulence. compared with all known sequence of ndv. there was a special sequence ( 5 ' tctctctcctctctcctcc3 " ) in the genomic cdna of ndv f48e9 strain
通過rt - pcr方法擴增獲得了另外10個背景清楚毒株的np - p基因間隔區片段,將這些序列與f48e9 、 lasota 、 clone30 、 b1 、 zj1和v4的相應序列進行了比較,結果在參比的16個ndv毒株中在該區段中除了有多個點突變外,個別毒株有堿基插入和缺失,所有以lasota株為代表的弱毒株均無6堿基的插入,而以f48e9株為首的強毒株均有此6堿基的插入,但有一個中等毒力的毒株dp沒有6堿基的插入,不過它的基因序列和lasota的幾乎相同,對于所克隆到的基因的代表性還有待確定。The genetic diversity of p. sibirica, which is represented by 98 individuals collected from all 5 subpopulations, investigated with rapd markers. 77 loci were selected by fourteen 10 - mer primers, of which 76 were polymorphic. the percentage of polymorphic loci ( ppb ) of five subpopulations was 70. 13 %, 77. 99 %, 62. 34 %, 67. 53 %, and 61. 04 % respectively
用rapd分子標記對叉毛蓬5個亞居群共98個個體進行了遺傳多樣性檢測, 14個10堿基寡聚核甘酸引物共檢測到77個位點,其中多態位點76個,多態位點比率為98 . 7 。The optima technologic conditions of preparing anthracite - based hsaac by orthogonal test are ratio of koh to anthracite 4, activating temperature 750 c and activating time 1. 5 hours respectively
由正交試驗結果確立的制備無煙煤基hsaac的最佳工藝條件為:堿炭比為4 ,活化溫度為750c ,活化時間為1Comparing thl2 dna encoding region of b. olereacea l. with that of b. napus l., 4 different bases were found, and three of them led to the alteration of amino acids
比較甘藍和油菜的thl2dna ,發現在編碼區有4處堿基不同,其中有3個堿基的突變造成了氨基酸的變化。Sequencing the variations in 200, 000 base pairs is a far easier task than reading a full genome ? more than three billion in all
決定20萬堿基對里的序列變異,要比讀取整個基因組簡單得太多,後者的數目可是超過了30億。Sequence analysis showed that, this fragment has a homology of 99 % to the previously reported choe from rhodococcus equi. after cloning and subcloning and three identical fragments were obtained from three independent pcr, lacking three continual bases ( ttc, encoding the pheamino acid ) compared with choe, thus it can be assumed that the new fragment, designated as choew, and choe belong to the same gene family, even it might be the natural mutation of choe
對片段進行克隆、亞克隆之後測序分析,發現與已克隆的來源於馬紅球菌的膽固醇氧化酶基因cboe有99的同源性;並且三次獨立的pcr均得到相同的片段,所克隆的基因序列與choe相比,連續缺失三個堿基( ttc ) ,即相應的氨基酸序列缺失一個氨基酸( phe ) ,因此判斷所克隆的基因片段與choe屬同一基因家族或原有基因的天然突變體,命名為choew 。Relatively, the new wibdv strains gx8 / 99 had less homology to wibdv reference strain hk46 and other 3 field strains as 96. 8 % - 97. 2 % at dna or aa levels, than the homology among hk46 and 3 strains, strains gx8 / 99 more than 98. 4 % - 98. 6 % at dna or aa levels
為研究病毒的核酸分子結構與其致病性的關系,本研究選取了在致死率上不同的4個ibdv野毒株,比較了它們的vpz基因高變區共494個堿基序列。The close genetic relationship of goose parvoviruse and aav allows the examination of the molecular biological properties of the nonstructural proteins of gpv. after the gpv infected the cell the viral life cycle was regulated by the nonstructural proteins encoded by the virus. according to the published of gpv b strain genome nucleotide sequences in genbank and a pair of specific primers were disigned with oligo4. 1
本研究根據genbank發表的gpvb株全基因序列,藉助oligo4 . 1軟體設計一對引物,採用pcr技術擴增gpvh1株非結構蛋白ns2基因,並與pmd18 - t載體連接后測序,結果表明:鵝細小病毒h1株ns2基因核苷酸全長1356bp ,編碼451個氨基酸殘基,與gpvb株的ns2基因相比,核苷酸數目相同,有17個堿基、 6個氨基酸的差異;同源性分析表明:二者核苷酸序列同源性為98 . 75 ,推導氨基酸序列同源性為98 . 67 。Through the study and analysis conclude as follows : in human dna sequences, if the distinction of the number of the four bases is very small, they can distribute in a sphere whose radius equal to 1 / 4 ; if the distinction of them is comparative large, they can distribute between the sphere and the frustum - octahedron ; if one is 0 in them, they can distribute between the frustum - octahedron and regular - octahedron ; if the distinction of them is especial large, they may distribute in the outer of the regular - octahedron
通過研究和分析得到結論為:在人類dna序列中,如果四個堿基的數目相差很小時,它們可能分佈在半徑為1 / 4的球內;如果相差比較大時,它們可能分佈在這個球和截角八面體之間;如果在四個堿基數目中有一個為零時,那麼它們有可能分佈在截角八面體和正八面體之間;如果相差的特別大,甚至有可能落在那個正八面體的外部。分享友人