堿核蛋白 的英文怎麼說
中文拼音 [jiǎnhédànbái]
堿核蛋白
英文
basonuclin-
G gene of rabies virus m, the of two main regions ( about 1000nt ), ranging from 3161nt to 4162nt and ranging from 4012nt to 4863nt of glycoprotein gene of rabies virus strain m, isolated from mouse in he nan, china were amplified by reverse transcriptase - polynerase chain reaction ( rt - pcr ) in order to complete glycoprotein gene of strain m. these regions were sequenced by the produce of pcr directly. comparison and analysis of nucleotide sequence and amino acid sequence deduced with that of other strains published was performed by computer with dnasisv 2. 5demo software
本研究對我國河南某地野鼠體內分離的狂犬病野毒株mrv基因的3161位? 4162位（ 1001個堿基）和4012位? 4863位（ 851個堿基）片段進行了反轉錄pcr擴增和序列測定，得到mrv的糖蛋白基因全序列，用dnasisv2 . 5demo分析軟體，與已發表的代表性毒株g基因全序列進行核苷酸和氨基酸序列的比較分析，結果表明在同一基因型中， mrv和國際標準攻毒株cvs的同源性最高（ 96 . 5 ） ，和中國減毒株ctn的同源性最低（ 79 . 8 ） 。Histidine a basic amino acid formed only rarely in proteins. it is formed from atp and 5 - phosphoribosyl pyrophosphate in a complex biosynthetic pathway. glutamate is an intermediate in its breakdown
組氨酸：是蛋白質中少有的一種堿性氨基酸。由atp和5 -磷酸核糖基焦磷酸鹽通過復雜的生物合成途徑合成。在其降解過程中可形成谷氨酸。To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei, and to study the function and mechanism of mint - mediated transcription repression, yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5, amino acids 2226 - 2959 ). from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo, fifty - one were positive for nutritional screening and p - galactosidase assay. restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments, which are mint, alpha a - crystallin - binding protein i ( alphaa - crybpl ), and nuclear receptor co - repressor 1 ( n - corl ), respectively
鑒于mwt基因編碼區較長，共有10799個堿基，故此我們將mint分為六段，分別命名為fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段為研究對象，將h者分別插入pgb盯7載體中，結果顯示： mintfs可與核受體輔助抑制因子1階cori ） ， o晶狀體蛋白結合蛋白1hlphatcrybp入及mw加互作用，而f6可與igm的重鏈恆定區、泛素結合酶2l6和一個未知功能的新的蛋白基因進行結合。It is a very basic, single - chain protein with molecular weight about 14 kd. it shares 33 % sequence identity with bovine pancreatic rnasea and has structurally equivalent counterparts for the two histidines and one lysine that comprise the catalytic residues for ribonucleolytic activity
它是由123個氨基酸組成的分子量約為14kd的單鏈堿性蛋白質，與牛胰核糖核酸酶有33的同源性，兩個組氨酸和一個賴氨酸殘基組成了血管生成素核糖核酸酶活性中心。Then, a piece of degenerate primer was designed according to the conserved amino acids of glycine betaine abc transporter system glycine betaine - binding protein as a reversed primer. combined with the opuaa - up, a 2. 3 kb fragment was obtained through pcr. blast result showed a fragment which contained the partial opuaa, the whole opuab and partial opuac sequences were obtained
再次，根據甘氨酸甜菜堿atp轉運系統底物結合蛋白的氨基酸保守序列設計下游簡並引物，與atp結合蛋白的上游簡並引物組合，經pcr擴增獲得2 . 1kb的條帶，測序后通過blast比較，結果顯示獲得atp結合蛋白基因的部分序列、通透酶的全部編碼序列和部分甘氨酸甜菜堿結合蛋白基因的核苷酸序列。It has been shown that domain ia is responsible for cell recognition, domain ii is to be involved in translocation of the toxin across membranes, and domain hi catalyzes the adp - ribosylation of elongation factor2, which arrests protein synthesis and results in cell death
人組蛋白h3是堿性核蛋白，富含精氨酸，在生理條件下， h3的精氨酸帶正電荷，而dna的磷酸基團帶負電荷，所以組蛋白和dna分子主要依靠靜電引力相結合。The close genetic relationship of goose parvoviruse and aav allows the examination of the molecular biological properties of the nonstructural proteins of gpv. after the gpv infected the cell the viral life cycle was regulated by the nonstructural proteins encoded by the virus. according to the published of gpv b strain genome nucleotide sequences in genbank and a pair of specific primers were disigned with oligo4. 1
本研究根據genbank發表的gpvb株全基因序列，藉助oligo4 . 1軟體設計一對引物，採用pcr技術擴增gpvh1株非結構蛋白ns2基因，並與pmd18 - t載體連接后測序，結果表明：鵝細小病毒h1株ns2基因核苷酸全長1356bp ，編碼451個氨基酸殘基，與gpvb株的ns2基因相比，核苷酸數目相同，有17個堿基、 6個氨基酸的差異；同源性分析表明：二者核苷酸序列同源性為98 . 75 ，推導氨基酸序列同源性為98 . 67 。In this study we firstly determined the time when the chitinase of helicoverpa armlerra produced in high activity and we cloned a cdna encoding a chitinase of helicoverpa armlerra by race method which was about l. 6kb and included complete 5 ' - end cap and 5 ' - utr
利用計算機分析該核昔酸序列，可知其編碼框為1341個核昔酸，編碼50kd含446個氨基酸的蛋白，推導等電點為phi9 575 ，為堿性蛋白。9 expression of mxmybl gene in e. coli was experimented. the structure of its recombinant expression vectors had not base mutant and drift. sds - page of pet - mxmybl proteins showed that there was a strengthened protein band in expectant 38kda protein marker
構建的原核表達載體結構正確，未出現堿基突變及移碼現象； lmmoffeiptg誘導zh后，在預期的蛋白分子量約38kda處出現一條表達加強的蛋白帶。Analysis of sperm nuclear basic proteins in 1 050 infertile men
1050例不育男性精子堿性核蛋白的分析分享友人