堿裂解 的英文怎麼說
中文拼音 [jiǎnlièjiě]
堿裂解
英文
alkaline lysis-
An improved alkaline lysis adaptive to extracting plasmid dna of bacillus
提取的改良堿裂解法Effect of agitation rate on batch alkaline pectate lyases fermentation by bacillus subtilis
分批發酵堿性果膠裂解酶的影響So some methods suitable to large plasmid extraction, including lysis by sds and a method from a literature, were used to try to extract the large plasmid from the strain cell. the lysis reactions in these two methods are gentle, so the large plasmid cannot be injured in the lysis process, opposite to lysis by alkali. it would be helpful to keep the integrality of the large plasmid during the extraction
因此我們採用了適合於大質粒提取的sds法，和文獻中應用於硝基苯降解性質粒的提取方法，來嘗試對菌株細胞進行質粒提取，這兩種方法裂解反應溫和，不會像堿裂解法那樣，在裂解過程中損壞質粒，可以實現質粒提取的完整性。The plasmids of the three bacteria were identified, the results showed m8 had n ' t plasmid, the plasmid of m6 and ml3 were about 20kb
用堿裂解法提取質粒dna ，結果發現： m8沒有檢測到質粒， m6和m13的質粒大小都為20kb左右。Methods : plasmid profile analysis was used
方法：堿裂解法。The presence of plasmid in bacillus was isolated by improved alkaline lysis. only one plasmid pbl29 was detected in bacillus iicheniformis29
本文採用改良堿裂解法對30株益生芽孢桿菌進行質粒抽提，僅從一株地衣芽孢桿菌bThe plasmid extraction from wild strains needs an appropriate method. first, lysis by alkali was used to extract plasmid from strain pseudomonas xn - 1. but the expected result had not been got, and a lot of cracked plasmid dna fragments were got only
對於野生菌株的質粒提取，需要選擇合適的提取方法，我們首先採用了細菌細胞質粒提取常用的堿裂解法，但是未取得理想的效果，只得到了許多破碎的質粒dna片段。Chapter 2 screening of genes related to the ovary development of the mitten crab ( eriocheir japonica sinensis ) by cdna macroarray analysis plasmids were extracted from all the clones in the subtracted cdna libraries with alkali lysis method, then the inserted fragments in plasmids were amplified by pcr
2用cdna微陣列篩選中華絨螯蟹卵巢發育相關基因將差減cdna文庫中的所有克隆用堿裂解法提取質粒，然後以提取的質粒為模板，用pcr方法擴增插入片段。Methods : the rearranged gene fragment coding tcr y v region of the jurkat cell line was obtained by rt - pcr technique the pcr product was cloned into the eukaryocytic expressive vector pcdnas to construct pcdna3 / tcr y. after confirmed by sequncing. pcdnas / tcr y plasmids were amplified in bacteria extracted by alkaline lysismethod
方法：本文採用rt ? ? pcr的方法擴增jurkatt淋巴瘤細胞特異性重排的tcr可變區基因片段，克隆到真表達載體pcdna _ 3中，經序列測定無誤后，堿裂解法大量提取質粒，制備dna疫苗。A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge
用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接；用連接產物轉化大腸桿菌jml09感受態細胞，置含amp 、 x - gal和iptg的lb平板上培養12 20小時；挑取白色菌落於選擇性培養基擴大培養，堿裂解法小量提取質粒dna ，並進行酶切分析鑒定，結果獲得整合有prvge基因的重組質粒pugedna ，並與其它prv分離株進行ge基因序列同源性分析。Abstract : 1, 2 - bis ( alkylphenylphosphino ) ethane was synthesized via the reaction between lithium alkylphenylphosphide and 1, 2 - dichloroethane with tert - phosphines as raw materials. their structures were established by ir, h1nmr spectroscopy
文摘:以叔膦為原料，經堿金屬裂解后與1 ， 2 -二氯乙烷反應合成1 ， 2 -雙膦乙撐化合物.研究了叔膦化合物的裂解方式，堿金屬膦化物的穩定性及對反應條件的影響.產物的結構由ir譜和h1nmr譜證實As the surface deteriorates, water in any form, dew, humidity, rain, wash and rinse water, as well as other microscopic destructive elements such as acids and alkali substances, enter into the microscopic pores, fissures, cracks and other surface imperfections and begin to attack, or breakdown the molecules that make up your car ' s paint
隨著漆面的惡化，水、露水、濕氣、下雨、清洗和漂洗的水，還有其它看不見的具有破壞性的物質如酸雨、堿性物質，都會浸入到小孔、裂縫、裂紋和其它表面不完整的地方也開始變化或者分解車漆的分子。分享友人