4. engineering dhqase ( arod ) - deficient e. coli mutant with a second copy of the arob gene gene targeting technique was used to disrupt the arod gene in e. coli chromosome. the mutant 31bk was engineered, in which homologous recombination of the arobkanr gene cassette into the arod locus ( arod : : arobkanr ) of the e. coli strain atcc31884 genome utilized the helper plasmid pkd46 with red system. the host cell 31bk lacked catalytic activity of dhqase ( arod ) and had a second copy of the arob gene, so it improved carbon flow into the quinic acid biosynthesis direction
構建宿主菌基因精確定位突變株31bk （ arod : : arobkan ~ r ）為了改變代謝途徑脫氫
奎尼酸（ dhq ）分支點上的代謝流量，使之充分流向目的產物
奎尼酸合成方向，
利用基因打靶技術構建了31884宿主菌arod基因精確定位插入突變體，使dhq脫水酶（ dhqase ）失活，阻斷了碳代謝流流向芳香氨基酸生成的方向，同時用同源重組的方法將arob基因定位整合入染色體上，解除了限速酶對碳代謝流通過共同途徑到達dhq的阻遏影響，並減輕代謝負擔。
Quinic acid, used shikimate pathway in e. coli, it is necessary to extend metabolic pathway by introduction of a heterogenous gene qutb into the host cell. double specific enzyme genes arog, qutb or three ones arog, qutb, arob were co - expressed in a single plasmid pbv220 to improve the enzymes " rate - limiting reactions. modifications of e. coli chromosome by both disruption of the arod gene and directed - site insertion of the arob gene resulted in the change of carbon flow redirected into the quinic acid biosynthesis branch
利用大腸桿菌莽草酸途徑合成新的代謝物
奎尼酸，須在宿主細胞引入異源酶基因擴展代謝途徑；串聯表達酶基因，同時適量增加不同種屬的多個關鍵酶酶量，改善限速反應；
利用同源重組進行基因整合和基因破壞，改造染色體結構定向改變微生物代謝途徑；目的是將碳代謝流最大程度的引向
奎尼酸生成的方向。
The total public for titian in the 16th century or velazquez in the 17th was probably no more than a few thousand people - though that included most of the crowned heads, nobility and intelligentsia of europe
16世紀的提香（義大
利畫家）或是17世紀的維拉斯
奎茲的畫迷大概也沒有超過1000人盡管這些人包括了歐洲大部分的皇宮貴族以及知識分子。
As she launched her latest movie, the fine art of love - mine haha, in venice, actress jacqueline bisset attacked the attitudes of women who " viciously " exploit each other
傑
奎琳?比塞特在威尼斯發布了她的最新力作《愛情的藝術》 ，同時，她還抨擊了影片中所講述的女性相互之間的惡意
利用。
Adrian, julian, brenna, ian, claire, and quinn are in their freshmen year of high school in indianapolis and loving life
艾德里安，朱
利安，布來，伊恩，克萊爾，
奎正在印第安納波
利斯享受他們的高中生活。
The ironforge exchange of qeynos has taken advantage of the recent bell, broker, banker and mender additions to qeynos to acquire the struggling business of fhara ' s furnishings in north qeynos, and has refurbished this building to be their new headquarters
鐵煅交易所因為新增加的水手之鐘、中介商、銀行以及修理工，為工匠們提供了更多的便
利，並且將店面重新翻修，在與北
奎的法拉家居的商業競爭中實力大增。
This paper suggests that there is another better way to judge whether a system is stable or not - that is analyzing the amplification of loop circuit with the nyquist rule
由於激勵信號是復數形式的變化量，因此我們還可以採用奈
奎斯特準則，
利用奈
奎斯特圖在復平面上的變化特點，對環路增益進行分析。
It has thus passed from asia ( u thant of burma, now myanmar ), to western europe ( kurt waldheim of austria ), to latin america ( javier p rez de cu llar of peru ), to africa ( boutros boutros - ghali of egypt, who served one term, and kofi annan of ghana, the current secretary - general )
因此，這個職位從亞洲（緬甸的吳丹） 、西歐（奧地
利的庫爾特?瓦爾德海姆） 、拉丁美洲（秘魯的哈維爾?佩雷斯?德
奎利亞爾）輪到非洲（埃及的布特羅斯?布特羅斯-加
利（一屆任期）和現任秘書長，迦納的科菲?安南） 。
The gods began to leave the chambers, but quellious lingered
諸神開始離開房間，但是
奎利業斯並沒有馬上離開。
When quellious had finished, erollisi marr nodded. " it would be an acceptable compromise.
奎利業斯剛說完，伊洛斯瑪爾就點頭道「這是個不錯的折中方案。 」
" i do, " replied quellious. " but i have another proposal to share with the two of you to ensure our interests are preserved.
「是的， 」
奎利業斯回答道， 「但是我還有另一個提議來與你們兩位分享，一個可以保障我們的
利益不被損害的提議。 」微妙的微笑掛在她的唇邊。
Searching for the meaning of theological language
麥奎利的語言哲學簡論
