子序列表 的英文怎麼說
中文拼音 [zixùlièbiǎo]
子序列表
英文
subsequence table-
Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method
利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。( 2 ) at translation level plant mutual sequence of starting translation aaca was added to start codon of t - pa gene by pcr ampliation and plant expression vector pbet was constructed
（ 2 ）在翻譯水平上通過pcr擴增的方式在t - pa基因起始密碼子處添加了植物翻譯起始共有序列aaca ，構建了植物表達載體pbet 。Methods : an artificial gene for fl extracellular domain cdna was synthesized by using favored genetic codons of pichia pastroris. by inserting human fl extracellular domain cdna coding 156 amino acid resi dues into pichia pastoris expression vector ppic9k containing aox1 promoter and the sequences of alpha secreting signal peptides, a recombinant expression plasmid ppic9k - fl was constructed, and integrated into the alcohol oxidase region of the host genome
為了提高外源基因的表達量，我們根據畢赤氏酵母偏愛密碼子人工合成了編碼fl胞外區156個氨基酸的cdna序列，目的序列被定向克隆到酵母分泌型表達載體ppic9k質粒上，構建ppic9k - fl表達質粒。The blastn results show that gyn - 15 is closely related to a symbiont of anemones, s. californium, and the free - living strain, gymnodinium varians. sequence comparison show that the similarities among each part of the sequences from these three strains are all above 99 %. phylogenetic reconstruction with neighbor - joining ( nj ) method using sequences of variable regions ( v1 + v2 + v3 ) of ssu rdna indicated that gyn - 15, s. californium and g. varians form a new clade with 100 % bootstrap support
以ssurdna序列中的三個可變區（ vz + vz + v3 ）和鄰接法困eighbor一joiningmethod ， nj法）構建共生甲藻屬的系統進化樹表明， gyn一巧與5 . cal扣rnium和g . varian :在共生甲藻屬內構成一個獨立的、自檢支持百分率為100 %的子類群（ clade ） ，根據這些結果可將gyn一巧初步鑒定為屬于共生甲藻屬。These results suggested that higher accumulation of ergosterol might be induced by imazalil in the isolate of pd07, which contained 4 - 126bp tandemly repeated in cyp51 gene more than that in the pd23
與敏感菌株相比，抗性菌株cyp51基因啟動子上游多了4個126bp的串聯重復序列，推測該重復序列可能是導致cyp51基因過量表達的內在原因。So we consider five financial indexes includes stock b / p, e / p, current stock size, current stock stru and financial levge by the international tradition, then descriptive statistical test method and cross section statistical test method proved that b / p and current stock size have marked effect on the securities yield besides coefficient b. in the third chapter, the article fut forward a risk factor model, estimates yield sequences of every risk factor by weight regression, and then estimates each risk factor coefficient of different stock by time sequence regression, at last we can reckon the portfolio risk o2p and yield rp which consists n stocks
結合國際慣例，文章考慮了股票的凈值市價比（ b p ） ，市盈率倒數（ e p ） ，流通規模（ size ） ，流通比例（ stru ）和財務杠桿（ levge ）等五個財務指標，應用描述性統計檢驗和橫截面統計檢驗等多種方法，結果表明，除系數以外，凈值市價比（ b p ）和流通規模（ size ）對證券收益率部有重要的影響。在論文的第三章，提出了一個基於多因素的風險因子模型，並用加權回歸和時間序列回歸等方法估計出了不同證券的各風險因子系數（類似於單指數模型中的系數） ，據此，即可衡量出一個包括n只股票的組合的風險_ p ~ 2和收益率r _ p 。In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into
經限制酶消化和dna序列分析，證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列，在iptg誘導下兩種嵌合分子都獲得了分泌表達，表達產物主要集中在細胞周質空間。Notch interaction with its ligands induces the cleavage of its intracellular domain ( ic ), and the notch ic translocates to the nucleus and binds to rbp - j, the mammalian homolog of su ( h ), to transactivate transcription of target genes such as e ( spl ) ( enhancer of split ), hesl ( hairy and enhancer of split ) and hes5 four notch receptors and their ligands are differentially and redundantly expressed in a variety of vertebrate tissues
它通過其識別序列（ cogtgggaa ）結合於受調控基因的啟動子區，在轉錄激活因子的驅動下調節細胞分化和個體發育相關基因的表達。在沒有n 。 tch胞內段的情況下， rbpj可與包含sm盯（ silencingmediatorforretlnoldandthyroidhormonereceptor ）和組蛋白去乙酞化酶的轉錄輔助抑制復合物結合，當notch信號被激活時； rbpj可與n 。To test the p7 promoter activity, a series of constructs were obtained by cloning the different dna fragments into the luciferase gene reporter vector pgl3 - b. when the constructs were transiently transfected into thp - 1 cells, luciferase activity assay showed that the core region of p7 promoter located at - - 165 bp
第一部分的工作首先通過對人acat - 1基因p7啟動子序列進行5 '和3 ' -端的缺失的詳細分析，結果顯示最大報告基因轉錄表達活性位於- 612 - 165bp區段。Besides, vgb gene expression also increased the chitinase secretion. in order to make the vitreoscilla hemoglobin gene express in bacillus subtilis, an inducible promoter ( levansucrase ) was cloned from b. subtilis wb600 and ligated with a promoter - less vgb gene. the resulted gene is called sacvgb and was demonstrated to express in e, coli by sds - page and carbon monoxide binding assay
由於vgb基因啟動子不能在枯草桿菌中啟動表達，因此，根據已發表的果聚糖蔗糖酶基因（ sacb ）序列設計引物，從枯草桿菌wb600總dna中擴增出該基因的啟動子片段，然後將其與vgb基因編碼區及終止子序列相連，成功地組建了sacvgb融合基因。In the study, several recombinant expression vectors were constructed in vitro by using the technique of gene engineering, making pheromone 3 be expressed under the control of different promoters and signal pcptides
本研究利用基因工程的方法，體外構建重組表達質粒，探索用不同的啟動子和信號肽序列表達八肋游仆蟲信息素3蛋白。The results showed that the sequence of two strains basically coincided but differenc
因此， biob與biof基因間終止子序列的刪除，不影響基因的表達。Used long pcr primers as sequencing primer, the two sides of sequence of the long pcr product were separately sequenced one reaction by the direct sequencing method of pcr products, and the sequencing sequence were carried on constrasted analysis with sequence of 168 strain in genebank
一2片段克隆質粒為模板，採用skippcr方法，得到了約4 . 3kb產物，以ta克隆方式，插入pgem一t載體中。將經鑒定的陽性克隆質粒進行測序，結果表明: biob與biol基因間的5lbp的終止子序列已被刪除。Zhonghua 11 ) at different growth phases. so, the promoter region ofosdd2 gene was cloned and fused with a gus gene, in order to speculate the osdd2 expressing patterns
鑒于基因表達模式不清，克隆了osdd2基因約2kb左右的啟動子序列並與gus基因融合，構建植物轉化載體。Ordered list of child information items, in document order
按照文檔順序的子元素信息的有序列表By using the method of genome walking, two fragments were cloned from genome walker library after the semi - nested pcr. the result of sequencing the bigger fragment showed that this 706bp sequence contained the atg start codon
通過半巢式的基因步移法，經二輪pcr后，從genomewalker文庫分離到2個片段，較大的片段測序表明該706bp序列含有編碼ast的起始密碼子atg ，其上游序列為啟動子序列。Think about how to write up your experience in targeted, clear, bulleted, detail - rich prose. here are some examples
想想怎麼寫了，你的經驗，在有針對性的，明確的，無序列表，詳細豐富的散文。這里是一些例子。The only exception is for nested ordered lists, which are placed in substeps
惟一的例外是嵌套有序列表，它被放在子步驟中。This comparative analysis, in which the algorithms predict overlapping, although not identical, sets of factors, argues for meticulous benchmarking of promoter sequence analysis methods to determine the positive and negative attributes that contribute to their varying results
三者有交叉但不完全相同，表明選擇適宜的啟動子序列分析方法為基準對鑒別導致其不同表達特徵的正負屬性至關重要分享友人