寡克隆 的英文怎麼說
中文拼音 [guǎkèlōng]
寡克隆
英文
oligoclonal-
Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed
本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。A pair of primers was designed based on the conserved regions of other higher plants " epsp synthase through homology alignments. two dna fragments were first cloned from o. violaceus by performing prc which used o. violaceus genome as the template. one has 798 nucleotides and the other 1157 nucleotides, but they can encode the same amino acid sequence and have same extrons according to the gt - ag rule of characteristic sequence of enkaryoutic intron
根據同源比較其它高等植物中epsp合成酶基因，找出該基因的保守序列並設計一對寡核苷酸引物，以諸葛菜的總dna為模板進行pcr反應，克隆出了兩個epsps基因的片段，其中一條長為797bp ，另一條1157bp ，它們在genbank的登錄號為： af440390 、 af440391 。The lesions can be seen with mri scans, but the appearance in the csf of increased protein from igg that demonstrates oligoclonal bands on electrophoresis is very consistent with this diagnosis
採用磁共振（ mri ）掃描可發現損害，腦脊液檢測結果為免疫球蛋白g升高，這表明電泳顯示的寡克隆帶與診斷是非常一致的。And the two pah ' s of pcr primers that bind to the adapter and the sequence of f fragment close by tn5 respectively were also designed. the genomic dna of b8 was isolated, digested with bamh i, and ligated to the adapter. using the two pairs of the primers, two rounds of pcr were performed hi turn and a fragment of 239bp was amplified successfully. lt was proved by cloning and sequencing that 18bp of the fragment is the sequence opposite to f fragment on the left of tn5 insertion site in b8f, the other is part of the 728 bp of f fragment. this result makes it possible to continue to carry out chromosome walking, to clone and sequence the whole genes of b fragment and f fragment, and to reveal the antagonistic molecular mechanism of b8
試驗研究設計併合成了由40和44個堿基的寡聚脫氧核苷酸組成的染色體爬行接頭，在接頭序列和測定的f片段近tn5的序列上，設計了2對染色體爬行用的pcr引物，從b8菌株中提取基因組dna ， bamhi酶切，與染色體爬行接頭連接，依次用2對引物進行pcr ，擴增出239bp產物，經克隆、測序，發現其中18bp為擴增的相應于f片段在b8f菌株tn5插入位點對面的序列，其餘則為f片段728bp序列的一部分，為進一步進行染色體爬行，克隆和測定整個b和f基因，揭示陽菌株的拮抗分子機制提供了技術資料貯備。分享友人