嵌合胚 的英文怎麼說
中文拼音 [qiàngěpēi]
嵌合胚
英文
chimeric embryo-
Part 1 : the culture and identification of es - d3 cells and the study of the efficiency of eb formation from es cells when grown on mef feeder layer in es culture medium or cultured in es culture medium supplemented with lif 1000u / ml, es - d3 cells being used in our experiments formed normal clones, expressed akp and kept their normal karyotype over many passages. the in vitro and in vivo differentiation experiments showed that es - d3 cells could differentiate into variety of cell types derived from three primary germ layers
結果顯示: eso3細胞在小鼠胚胎成纖維細胞上和或含白血病抑制因於億f )的es細胞培養液中形成典型的胚胎幹細胞克隆,堿性磷酸酶染色結果為強陽性,具有正常二倍體核型以及具有在體內外分化為三個胚層來源的組織細胞的潛能,而且具有形成種系嵌合動物的能力。Two stage embryos were observed their effect on production of chimera. the result shows that there is a significant different ( 0. 01 < p < 0. 05 ) between blastocysts ( including early blastocysts and morulae ) and expanded blastocysts
結果表明,囊胚、早囊及桑椹胚同擴張囊胚兩種注射胚胎製作嵌合體所產生的結果差異顯著( 0 . 01 p 0 . 05 ) 。Multi - locus dna fingerprint technique was used to check the chimerism of chimeric mouse generated by injecting es cells into blastocysts and to detect whether the chimeric mouse is a germ - line chimeras. the results indicated that : the multi - locus dna fingerprint with a new synthesized probe - jl - 02, has enough polymerism and good stability, and should be very useful to monitor the chimerism in different tissues of es cell chimeric mouse and to check whether an es cell line has the capacity to enter the germ line, especially when involving strains that can not be discerned with coat color or biochemical markers
嘗試應用多位點dna指紋技術,檢測經過胚胎幹細胞es細胞途徑所獲得的嵌合體小鼠中es細胞在各種臟器中的嵌合情況檢測es細胞在嵌合體小鼠中是否實現種系傳遞。結果表明:採用新型的人工合成的寡核苷酸多聚體探針jl - 02探針的多位點dna指紋圖譜,具有足夠的多態性和很好的穩定性。Different amount of copies in different tissues attribute to the different density of positive signals. the result of the experiment suggested that the transgenic animals can be produced by spermatozoa - mediated gene transfer after the entrapment of liposome. and because the exogenous dna occurs losing the segments. partly integration, or existin g outside of genome dna, the rate of chimerism is relatively high
結果表明: ( 1 )脂質體包裹外源基因轉染精子的方法,可將外源基因導入受精卵中,能夠獲得轉基因動物,並得到了較高的轉基因陽性率; ( 2 )精子攜帶的外源dna的整合過程是隨機的,在受精過程和胚胎早期分化過程中可能發生了片段丟失、不完全整合或游離于基因組存在而產生嵌合體。Hfea deferred a decision on other types of human - animal embryos, such as what are known as " true hybrids " ? created by the fusion of a human sperm and an animal egg ? and so - called " human chimeras, " where human cells are injected into animal embryos
Hfea延緩了針對另外一種類型的人-動物胚胎的決定,例如已為我們熟悉的「真雜交體」 ? ?通過融合人精子和動物卵細胞製造的雜交體? ?因此被成為「人嵌合體」 :人細胞被注射到動物胚胎中。164 microinjected embryos and 121 noninjected embryos were transferred to 14 recipient gilts. of this, 159 microinjected and noninjected embryos ( blastocyst, early blastocyst and morula ) was transferred to 6 recipient gilts, three pigs are pregnant ( one gilt delivered 8 piglets, two are skin pigment chimera ), and pregnancy and chimera rate is 17 % ( l / 6 ). 126 microinjected and nonmicroinjected expanded blastocysts oestrused on time, pregnancy and chimera rate is 0 ( 0 / 5 )
其中, 159枚囊胚、早囊及桑椹胚混合胚移植到6頭受體, 3頭妊娠(其中一頭母豬產仔8頭,兩頭為皮膚色素嵌合體) ,妊娠嵌合率為17 % ( 1 / 6 ) ; 126枚擴張混合囊胚移植到5頭受體均按時返情,妊娠嵌合率為0 ( 0 / 5 ) 。The results showed that es cells derived from kunming albino mouse remained undifferentiated state when they were cultured in vitro for a long time, and karyotypies were stable, but the ability of germ - transmission have not been verified
結果顯示,用昆明白小鼠囊胚所建立的胚胎幹細胞在體外長期培養時仍能保持未分化的狀態,核型穩定,但還未證明其具有生殖系嵌合的能力。One chimaeric mouse was produced by injection of es cells into blastocyst of c57bl / 6j. the chimaeric rate of fur colour was about 15 %. fl generations of germ - transmission derived from es cell were not obtained when chimaeric mouse mated with kunming albino mice and c57bl / 6j mice
將胚胎幹細胞注射到c57bl 6j小鼠的囊胚腔內,移植后產生了一隻wk2 -胚胎幹細胞的雄性嵌合體鼠,毛色嵌合率為15左右,成熟後分別與c56bl 6j和昆明白雌鼠交配,均未得到來自es細胞的後代。This study did microinjection to kunming white mouse inbred embryos to test its in vitro developmental ability, then pluripotency of porcine eg cells was tested with production of chimera by blastocyst injection. finally, first chimeric pig in china was delivered, and by chimera analysis, chimerism is present in some tissues
本研究首先利用近交系昆明白小鼠進行胚胎顯微注射觀察發育效果,然後利用所獲五指山豬eg細胞製作嵌合體檢驗eg細胞多能性,最終獲得國內首例胚胎幹細胞( eg細胞)嵌合體豬,經dna分析確認在多種組織中存在嵌合狀態。The researchers took a two - step approach : first they injected uniparental es cells into wild - type blastocyts to generate chimeric animals ; then they harested these chimeric fetal liers for transplant into lethally irradiated hosts
研究人員採取了兩步驟的方法:首先,他們將單系胚胎幹細胞注入野生型的胚細胞培育嵌合體動物;然後收集嵌合體胚胎肝臟移植入受致命性輻射照射的宿主。Experiment iii dna analysis of porcine embryonic stem cells ( eg ) chimera
試驗三,豬胚胎幹細胞( eg )嵌合體的dna分析。It may arise naturally due to mutation in a cell of a developing embryo, producing a line of cells with the mutant gene, and hence different characteristics compared to surrounding cells
嵌合體可以由發育著的胚胎細胞變異而自然形成,這樣就產生了具有不同性狀的變異基因的細胞系。In contrast to murine studies, studies of human hscs have relied on a number of in vitro assays that identify cells with the capacity to generate hematopoietic progenitors in long - term culture and to differentiation into multiple hematopoietic lineages. recently, two elegant animal models that enable the in vivo measurement of human hscs marrow repopulating potential have been developed. one is the sheep in utero transplantation system in which human hematopoietic progenitor cells are transferred into the sheep fetus in utero before the development of the ovine immune system
早在80年代中期, zanjani等就利用胚胎期免疫系統發育不完善,將人的造血幹細胞移植到妊娠中期胚羊腹腔內,成功地建立了人胎羊造血幹細胞嵌合體移植模型,並以此實驗模型作為體內檢測人造血幹細胞和人骨髓間充質幹細胞多向分化潛能的方法。分享友人