幼孢子體 的英文怎麼說
中文拼音 [yòubāozitǐ]
幼孢子體
英文
juvenile sporophyte-
By using primers designated for lacz gene, pcr result suggested an integration of lacz into u. pinnatifida genome. nine reagents including penicillin g, kanamycin, g418, teicoplanin, zeocin, chloramphinicol, hygromycin, basta and methotrexate were tested as selective reagents for selection of transgenic sporophytes. the results showed that young sporophytes of u. pinnatifida were sensitive to chloramphinicol and hygromycin and very sensitive to basta which suggest the potential of using the resistance genes as selectable markers
，為了解決轉基因裙帶菜的篩選問題，進行了裙帶菜幼孢子體對不同篩選壓力的敏感性實驗，包括抗生素類的青霉素g 、卡那黴素、 g418 、硫酸鏈黴素、鹽酸潔黴素、 zeocin 、氯黴素、潮黴素，除草劑類的basta ，氨甲喋呤類的氨甲喋呤，結果顯示裙帶菜幼孢子體對氯黴素、潮黴素敏感，對basta非常敏感。Israelensis recombinants, which contained recombinants plasmid pmt4 and pmt9 respectively, were obtained by electroporation. the bioassay results showed that the recombinants b - pmt9 and b - pmt4 had toxicities both to resistant and susceptible c. quinqnefasciatus larvae during vegetative growth stage, having the lc ? o values similar to that of. fi. sssii - 1. however, the toxic levels of the final sporulated cultures of recombinants b - pmt4 and b - pmt9 differed, with a lcso value of 2 49mg / ml for b - pmt9 and little toxicity for b - pmt4 by using the plasmid pmt9, m txl gene from b. sphaericus was ligated with p20 and cytjaa gene, giving recombinant plasmid pmpx2
含有pmt9和pmt4的大腸桿菌轉化子能表達產生mtx1毒素，發酵液對敏感和抗性致倦庫蚊幼蟲具有中度毒殺作用；含有pmt9和pmt4的蘇雲金芽孢桿菌轉化子b - pmt9和b - pmt4在營養體生長階段對敏感蚊幼和抗性幼蟲也具有毒性，毒力與野生型b . sss - 1相當，而不同轉化子在芽孢形成期的毒力因插入的mtx1基因轉錄方向不同而表現出差異，其中b - pmt4對目標蚊幼毒力極低（ lc _ （ 50 ） 10mg ml ） ，而b - pmt9對蚊幼蟲具有毒性（ lc _ （ 50 ） = 2 . 49mg ml ） 。Several vegetative clones of male or female gametophytes of u. pinnatifida has been induced and parthenogenesis of female gametophytes has been observed. 90 % of the gametophytes can grow into young sporophytes one month after fertilization. no staining background of either lacz or gus was found in different tissues of u. pinnatifida
進行裙帶菜配子體孤雌生殖、孤雄生殖和雌雄受精的再生途徑實驗，結果沒有得到孤雄生殖的裙帶菜，孤雌生殖的效率低、時間長且發育不正常，雌雄受精的再生途徑一個月左右90 %以上的雌配子體都正常萌發成幼孢子體。
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