循序點火 的英文怎麼說
中文拼音 [xúnxùdiǎnhuǒ]
循序點火
英文
train-type ignition-
A approximately 460bp dna fragment was amplified by pcr from lactococcus lactis nizo r5 genome. the primers used in this study comprised the following nucleotide sequences : 5 " - cgcgaattcgatataggtttattgagt - 3 " and 5 " - atgaagcttatccatgtcagaactaa - 3 ". conditions used for the pcr consisted of 30 cycles of 94 ? for0. 5min, 45 ? for imin and 72 ? for 1. 5min, plus one additional cycle of 72 for 10min
根據已發表的乳鏈菌肽前體基因的dna序列，設計兩條引物並引入酶切位點。以lactococcuslactisr5基因組dna為模板， pcr反應條件： 94變性30s ， 45退火60s ， 72延伸90s ，反應進行30個循環。成功擴出一條約460bp的dna片段。
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