愈傷組織分化 的英文怎麼說
中文拼音 [yùshāngzǔzhīfēnhuà]
愈傷組織分化
英文
callus differentiation- 傷 : Ⅰ名詞1 (人體或其他物體受到的損害) wound ; injury 2 (姓氏) a surname Ⅱ動詞1 (傷害) injure; h...
- 組 : Ⅰ名詞1 (由不多的人員組成的單位) group 2 (姓氏) a surname Ⅱ動詞(組織) organize; form Ⅲ量詞(...
- 織 : 動詞(編織) knit; weave
- 分 : 分Ⅰ名詞1. (成分) component 2. (職責和權利的限度) what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] (料想) judge
- 組織 : 1 (組織系統) organization; organized system 2 (組成) organize; form 3 [紡織] weave 4 [醫學] [...
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Relative physiological and biochemical features of redifferentiation difference in three types of calli subculture in anthurium andraeanum
花燭愈傷組織不同繼代培養的再分化差異In this study, the stem segments of new shoot with axillary buds of well - growth tetraploid black locust trees were used as explants. the effects of different basic mediums, different hormone kinds and their concentrations ratios, different sucrose concentrations on calli induction, buds differentiation and rooting in the process of establishment of high frequency regeneration system of tetraploid black locust were studied. on the base of high frequency regeneration system, the effects of various factors on transformation efficiency of badh mediated by agrobacterium tumefaciens were discussed in the light of gus histochemical assays
本實驗首先以生長良好的四倍體刺槐優株上當年生新梢的帶腋芽莖段為外植體,研究了在四倍體刺槐高頻再生體系的建立過程中不同基本培養基、不同激素濃度及其配比、不同蔗糖濃度對愈傷組織的誘導、芽的分化及生根的影響;然後在得到高頻再生體系的基礎上,通過農桿菌介導法轉化甜菜堿醛脫氫酶( badh )基因,以gus染色組織分析為依據探討了影響轉化效率的各種因素,建立了高效、可重復的基因轉化體系,為四倍體刺槐目的基因的導入打下了基礎。2. according to the effect of combination of different hormone concentration in the medium on callus formation and shoot induction of tomato cotvledons, we defined mso + 2. 0mg / l ba + - 0. 2mg / l iaa as optimum differential medium
根據番茄子葉外植體在加有不同激素濃度配比的培養基上愈傷組織分化和芽再生的情況,確定最佳分化培養基為ms _ 0 + 2 . 0mg / lba + 0 . 2mg / liaa ; 3Signals were strong in the cell periphery of procambium, and longitudinal signals were stronger than lateral ones ; in root ground meristem cytoplasm, concentration in the perinuclear region was stronger than one in the cell periphery. in cell periphery of root ground meristem, distribution of actin mrna was heterogeneous, longitudinal signals were stronger than lateral ones ; in callus meristem cytoplasm, concentration in the perinuclear region was low ;
這表明,從棉花愈傷組織薄壁細胞到鳥巢狀管胞團再到正常苗的過程中肌動蛋白mrna的分佈和濃度都有明顯的變化,而在這里愈傷組織在分化到鳥巢狀管胞團后就不再繼續發育,因而推測,肌動蛋白mrna分佈和濃度可能影響愈傷組織分化出正常的植株。Callus 1. a mass of undifferentiated parenchyma. if a plant is injured, the surrounding uninjured parenchyma cells form a suberin - impregnated callus, sometimes, sealing off the wound
愈傷組織: 1 .一團未分化的薄壁組織。如果植物受到損傷,那麼周圍未受傷的薄壁組織就會在傷口處形成具有分生能力的組織,有時可以封閉傷口。The relationship between the differentiation of rice callus and the proportion of different plant hormones
水稻愈傷組織分化與不同激素配比關系的研究We studied development mechanism by the distribution of microfilaments and actin mrna in cotton callus, healtny plants and abnormal plantlets. fitc - phalloidin as fluorescence probe was used to investigate the meristem of the cotton root, abnormal plantlets and callus that was unable to germinate into healthy plants
本研究選取正常棉花的根,已經培養了長時間不能分化出正常植株的棉花愈傷組織和棉花畸形苗為材料,採用石蠟切片,通過fitc -鬼筆環肽對材料微絲熒光染色,結合熒光顯微鏡觀察。There are also different in inducing cluster buds from callus of clone gingkgo and its other tissues and organs, some callus can induce cluster buds, but others can not. differentiation rate from the callus of cotyledon can be up to 36 % and the number of cluster buds is positive related to the times of subculturing
但是不同器官誘導的愈傷組織對于叢生芽的誘導也是不同的,子葉誘導的愈傷組織的分化率較高,有36左右,並且繼代次數的增加也能夠增加分化的幾率Pre - differentiation and proper partial desiccation of calli before transferred to regeneration medium was found to apparently improve the frequency and quality of plant differentiation. with our optimized culture condition and treatment style, induction frequency of pei ' ai64s and 9311 can be reached 62. 45 % and 85. 30 % respectively and after 3 months of subculture calli can remain high - quality embryogenic state, when high - quality embryogenic calli after two times of subculture were used as acceptor, callus differentiation frequency can arrive at 85. 5 % and 87. 7 % respectively
採用我們優化后的培養條件與處理方式,培矮64s和9311愈傷組織的誘導頻率分別可達到62 . 45和85 . 30 ;繼代培養時間達三個月左右仍能保持較好的胚性生長狀態;對于繼代兩次胚性生長狀態良好的愈傷組織分化頻率分別可達到85 . 5和87 . 7 。The suitable culture medium of bud induction was ba4. 0 mg / l + naa0. 05mg / l, which had the suitable callus quantity and the rate of shoots differentiation was 21. 2 %
蝟實愈傷組織不定芽誘導的最優激素組合為ba4 . 0mg l + naa0 . 05mg l ,其愈傷組織生長量適中,芽的分化率達21 . 2 。The results of its culture in vitro were as follow : young leaves were the best explants in callus induction, the suitable culture medium was ms + ba2. 0mg / l + naa0. 1mg / l. the results of organ differentiation were that ba was better than zt in the induction of shoots, but zt was better than ba in the induction of root and callus quantity
蝟實愈傷組織誘導以幼嫩葉片為外植體較好,培養ms + ba2 . 0mg l + naa0 . 1mg l為好。進一步誘導器官分化,結果發現ba對芽的誘導效應強于zt ,而zt在對根的誘導、愈傷組織生長量上優于ba 。When cultured in liquid medium, the growth period of a. ficoidea cv. " ruliginosa " hairy roots could be divided into three phases : lag phase, rapid - growth phase and plateau phase
而在添加6 - ba2 . 0mg l和naa0 . 8mg l的ms培養基上,部分愈傷組織能分化出幼芽。紅龍草毛狀根液體培養的生長過程分為三個時期:生長遲滯期、快速生長期、生長平臺期。In autograft from inflorescence stems callus tissue was found 4 days after grafting. no xylem and sieve elements were differentiated in both scion and stock in this period
C24擬南芥花序軸自體嫁接后4d ,嫁接面處愈傷組織開始形成,嫁接體雙方還沒有分化出管狀分子和篩分子。Hybrid embryos regenerated by callus and obtained more lily plantlets. isoenzyme zymograms of lily plantlets by embryos culture were similar to results of seed developed into plantlet
雜種胚通過愈傷組織途徑分化成苗,植株再生頻率高,初步鑒定,其同工酶譜帶與雜種實生苗是相似的。The cell division element class plant growth regulator is one kind of promotion plant being of ability to promote the vegetable cell fission and the variation, delay the leaf blade senescence, promote lateral bud growth, break the apical dominance, induce injuries of the organization, split up the different organs and promote chloroplast growth and synthesis
細胞分裂素類植物生長調節劑是一種促進型植物激素,能夠促進植物細胞的分裂和變異,延緩葉片衰老;促進側芽發育,打破頂端優勢;誘導愈傷組織,分化成不同器官;促進葉綠體的發育和合成。Studies on transformation of indica rice with bt - toxin gene mediated by agrobacterium tumefaciens precultured immature embryo and callus derived from young panicle, immature embryo and mature embryo were used as acceptor for genetic transformation mediated by agrobacterium tumefaciens, the transformation rate of the above acceptor was investigated respectively. the results showed that immature embryo after precultured for 4 ~ 6d was the best. in respect to the concentration of agrobacterium tumefaciens when calli were cotransformated in medium yeb, to agrobacterium tumefaciens eha 105, od value of 0. 8 was the best
採用農桿菌介導法將bt毒蛋白基因導入水稻同樣以上述兩種秈稻為主要研究材料,比較了分別以預培養的幼胚和幼穗、幼胚、成熟胚來源的愈傷組織作為轉化受體的愈傷組織轉化頻率,結果表明預培養4 6天的幼胚最適宜作為農桿菌介導轉化的受體;其次是來源於幼胚和成熟胚的生長狀態良好的胚性愈傷組織。Fig 2. differentiation of young shoots from transformed callus
圖2 :從基因槍轟擊過的愈傷組織分化出大量的小苗。Bap performed more important function than kt in differentiation of tall fescue embryogenic calli, but better results could be achieved with combination of 2mg / l bap and 0. 5mg / l kt. at this cytokinin level, 0. 5mg / l naa was recommended to obtain the highest callus regeneration frequency. plant regeneration could be evidently boosted when embryogenic calli were pre - differentiated on high - osmoticum medium with 60g / l sucrose, and / or when the pre - differentiated compact calli were differentiated on differentiation medium solidified with l0g / l agar
高羊茅胚性愈傷組織分化時, bap的作用要比kt大,但2mg lbap與0 . 5mg lkt配合可獲得更佳的效果;在該細胞分裂素水平下,生長素naa用0 . 5mg l ,愈傷組織再生率最高;胚性愈傷組織先在含60g l蔗糖的分化培養基上高滲預分化,以及經高滲預分化后的緻密愈傷組織在瓊脂濃度為10g l的分化培養基上分化,能明顯促進愈傷組織的植株再生;在分化培養基中添加脯氨酸導致愈傷組織再生率下降,但同時有減少白化苗再生的趨勢。An in vitro plantlet regeneration system was established from rhizome lumps in polygonatum cyrtonema hua and mannose - binding lectin in cultured rhizome was determined by sds - page and hemagglutination assays and also analysised by rt - pcr and comparison of the nueleotide sequences and amino acid deduced with natural polygonatum cyrtonema hua lectin ii.
利用野生囊絲黃精的幼嫩根狀莖作外植體,誘導形成愈傷組織,愈傷組織分化出不定芽,進而產生試管苗,試管苗經移栽試驗,成活率為75 ,其外觀性狀與野生植株無明顯差異。Mature embryo - derived calli of japonica rice ( oryza sativa l. ) cultivars nipponbare were transformed using agrobacterium tumefaciens strain agl1 carrying a binary vector pcas04 harboring the marker gene, neomycin phosphotransferase gene ( nptii ), driven by a promoter from the ubiquitin gene in maize, a promoterless p - glucuronidase gene near to the left border of t - dna for trapping gene and a strong promoter, rice actin - gb promoter, near to the right border of t - dna as activation tagging. in this system, co - cultivation was simplified, special selection stages and pre - regeneration stage were omitted, the whole process was almost under continuous light at 30 ? except co - cultivation and transgenic plants began to generate only after 7 weeks calli were induced
在一步轉化系統中,光照高溫條件下培養的水稻愈傷組織從誘導開始經過4周時間就可以達到轉化實驗的要求,並且簡化、優化了整個共培養過程,省去了一篩、二篩和預分化步驟,只用7周的時間就可以初步得到再生轉化植株;共191塊愈傷組織得到125塊抗性愈傷組織,轉化頻率達到65 . 4 ,最後共得到99棵獨立來源的再生植株,抗性愈傷組織再生頻率達到79 . 2 。分享友人