愈傷組織培養 的英文怎麼說
中文拼音 [yùshāngzǔzhīpéiyǎng]
愈傷組織培養
英文
callus culture- 傷 : Ⅰ名詞1 (人體或其他物體受到的損害) wound ; injury 2 (姓氏) a surname Ⅱ動詞1 (傷害) injure; h...
- 組 : Ⅰ名詞1 (由不多的人員組成的單位) group 2 (姓氏) a surname Ⅱ動詞(組織) organize; form Ⅲ量詞(...
- 織 : 動詞(編織) knit; weave
- 培 : 動詞1. (在根基部分堆上土) bank up with earth; earth up 2. (有目的地使成長、壯大) cultivate; foster; train
- 養 : Ⅰ動詞1 (供養) support; provide for 2 (飼養; 培植) raise; keep; grow 3 (生育) give birth to ...
- 組織 : 1 (組織系統) organization; organized system 2 (組成) organize; form 3 [紡織] weave 4 [醫學] [...
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Relative physiological and biochemical features of redifferentiation difference in three types of calli subculture in anthurium andraeanum
花燭愈傷組織不同繼代培養的再分化差異Embryological studies reveal that megaspore and such cells as egg, synergid and antipodals in mature embryo sac initiate the division of forming haploid plants through embryogenesis or callus formation
胚胎學觀察揭示大孢子與胚囊內的卵細胞、助細胞和反足細胞均有可能在培養中啟動分裂,通過胚狀體或愈傷組織形成單倍體植株。In this study, the stem segments of new shoot with axillary buds of well - growth tetraploid black locust trees were used as explants. the effects of different basic mediums, different hormone kinds and their concentrations ratios, different sucrose concentrations on calli induction, buds differentiation and rooting in the process of establishment of high frequency regeneration system of tetraploid black locust were studied. on the base of high frequency regeneration system, the effects of various factors on transformation efficiency of badh mediated by agrobacterium tumefaciens were discussed in the light of gus histochemical assays
本實驗首先以生長良好的四倍體刺槐優株上當年生新梢的帶腋芽莖段為外植體,研究了在四倍體刺槐高頻再生體系的建立過程中不同基本培養基、不同激素濃度及其配比、不同蔗糖濃度對愈傷組織的誘導、芽的分化及生根的影響;然後在得到高頻再生體系的基礎上,通過農桿菌介導法轉化甜菜堿醛脫氫酶( badh )基因,以gus染色組織分析為依據探討了影響轉化效率的各種因素,建立了高效、可重復的基因轉化體系,為四倍體刺槐目的基因的導入打下了基礎。Callus induction and culture of soybean
大豆愈傷組織的誘導與離體培養With 24 c or 4 c, the change trends of the content of the salidroside was basically consentaneous in the consecutive cultural eras of the callus. in the callus from the different explants, the influence was maximal to the the activity of pal enzyme and the influence was lowest to the the activity of ca4h enzyme, and the influence to the the activity of tal enzyme was ascertained according to the explant of the callus. so in the same explant with the different temperature or in the different explant with the same temperature, there was no incident between the content of the salidroside and the activity of enzymes pal, ca4h and tal, and we presumed that there may be emphasized particularly on different metabolic pathway of salidroside
( 2 ) 、愈傷組織的外植體來源、培養溫度條件和不同的繼代培養數都影響著其中紅景天甙的含量和苯丙氨酸解氨酶( pal ) 、肉桂酸解氨酶( ca4h )和酪氨酸解氨酶( tal )這3種酶的活性;不管是葉來源還是莖來源,不管是24培養還是4培養的愈傷組織,在連續繼代培養中紅景天甙含量的變化趨勢基本上是一致的;無論是葉來源的愈傷組織還是莖來源的愈傷組織,培養溫度對pal酶的酶活性影響最大,對ca4h酶的酶活性影響最小,對tal酶的酶活性影響視不同的外植體來源而定;在相同外植體來源的愈傷組織中及不同的培養溫度的條件下,或是在不同外植體來源的愈傷組織中及相同溫度的培養條件下,其紅景天甙含量與pal酶、 ca4h酶和tal酶的酶活性之間沒有完全一致的對應伴隨關系。2. according to the effect of combination of different hormone concentration in the medium on callus formation and shoot induction of tomato cotvledons, we defined mso + 2. 0mg / l ba + - 0. 2mg / l iaa as optimum differential medium
根據番茄子葉外植體在加有不同激素濃度配比的培養基上愈傷組織分化和芽再生的情況,確定最佳分化培養基為ms _ 0 + 2 . 0mg / lba + 0 . 2mg / liaa ; 3Cotyledon and hypocotyl ' s rate and quamity are the most among these explams, and callus can be obtained in 10 days by cotyledon and hypocotyl. reversely it is difficult to indue callus with root, and the callus from root is lnde and easy to become browning. the calius obtained from leaf grows very slow and does not become browning uniill in 2 or 3 months
銀杏的不同器官和組織都能夠誘導出愈傷組織來,其中,子葉和胚軸10d左右全部愈傷化,誘導速度和誘導率均最高,根則很難誘導,愈傷組織很少,褐化很快;葉片誘導的愈傷組織,生長慢,褐化也慢,在培養基上保持兩三個月而不褐化;胚乳的誘導時間也較長,需要30d左右。Call from strawberry anthers of darslect and toyonaka were induced on ms media with 6 - ba and 2, 4 - d, or 6 - ba and naa, and the inducing frequency was very high
當花藥在附加2 0mg l6 - ba和0 5mg lnaa的培養基上進行培養時,愈傷組織誘導率最高。Furthermore the expressions of all the genes in a representative sample were examed by the recently developed method of hybridization to cdna arrays. this was intended to strengthen the theoretical background for the screening of norway spruce genotypes with low lignin content. the calli of the transformed sublines a78 - 3, a78 - 4, a78 - 5 and the untransformed control a95 : 88 : 22 were successfully induced to form mature embryos from which plantlets were established
以轉基因亞系a78 - 3 、 a78 - 4 、 a78 - 5和未轉基因對照a95 : 88 : 22的細胞愈傷組織為實驗材料,以dkm和lp - m熟化培養基培養五周后,再以1 4sh萌發培養基培養四周,成功地誘導形成了胚胎,並再生成新的小植株,萌發成活率達到80 。Carrot tissue culture and plant regeneration factors including explants, medium and culture condition are combined together to study the most efficient protocol of carrot tissue culture and plant regeneration thereof. the most suitable explant is fresh hypocotyls segment and precultured hypocotyls derived from 7 - 10 day old aseptic plantlets generating in dark or in dim light, the best recipe for cullus induction and subculture is b5c ( 85 with 0. 5mg / l 6ba and 0. 5mg / l 2, 4 - d ), the ideal recipe for plant regeneration is 65 or ms free of hormone. a phytotron with a 16 / 8 h day / night cycle, at 25 is feasible for plant regeneration, and occasional exposure to sun light dramatically stimulates plant growth
建立了高效的胡蘿卜組織培養及再生體系以適于生產飲料的胡蘿卜「新黑田五寸人參」為材料,研究不同外植體、不同培養基,不同培養條件對胡蘿卜愈傷誘導及再生的影響,建立一套高效的胡蘿卜組織培養再生體系:最適于誘導愈傷的外植體是弱光或黑暗下發芽7 - 10d無菌苗下胚軸,最適合的愈傷誘導培養基和繼代培養是b _ 5c ( b _ 5 + 0 . 5mg l6ba + 0 . 5mg l2 , 4 - d ) ,最適于植株再生的培養基為不添加任何激素的b _ 5或ms ,組織培養條件為25 、光照周期為16hr 8hr 。Somatic embroyogenesis and plant regeneration from lyciun barbarun l suspension anther cell
枸杞花藥愈傷組織細胞懸浮培養與植株再生Study on callus culture and content test of baicalin in scutellaria baicalensis george
黃芩愈傷組織的誘導培養及其黃芩苷的含量測定Pepper ( capsicum annuum l. ) is one of the important vegetables and condiments. in order to laying down the foundation for the selecting and breeding of polyploid of pepper, a experiment of induction polyploids of pepper by colchicines treatment in clones of pepper was carried out
本實驗用萌動的辣椒種子在ms培養基中培養獲得無菌苗,取下胚軸為外植體,在ms + 6 - ba2 . 0mg l + iaa1 . 0mg l培養基中誘導形成愈傷組織。Callus culturing and cytomorphology observation of scutellaria baicalensis
黃芩愈傷組織培養及細胞形態學觀察We studied development mechanism by the distribution of microfilaments and actin mrna in cotton callus, healtny plants and abnormal plantlets. fitc - phalloidin as fluorescence probe was used to investigate the meristem of the cotton root, abnormal plantlets and callus that was unable to germinate into healthy plants
本研究選取正常棉花的根,已經培養了長時間不能分化出正常植株的棉花愈傷組織和棉花畸形苗為材料,採用石蠟切片,通過fitc -鬼筆環肽對材料微絲熒光染色,結合熒光顯微鏡觀察。Many researchers have conducted experimellts about it, but not succeeded. culture media were compared to find the best medium of gingkgo culture for controlling callus browning by different sugars, antioxidants and sorbents. the result showed that the medium with ms + zt 1. 0mg / l + naa 1. 0mg / l + sucrose 50g / l + agar 8g / l + ac 2g / l was the best medium, at l5 days subculturing intervals
銀杏組織培養過程中,尤其是在愈傷組織的繼代培養中,褐變現象特別嚴重,曾有不少的人做過這方面的研究,但都沒有成功,而本研究通過對不同糖類物質、抗氧化劑、吸附劑以及不同的培養基對褐變的影響和控制效果,探索出有效控制褐化現象發生的最佳培養條件,試驗結果表明: ms + zt1 . 0mg / l + naa1 . 0mg / l +蔗糖50g / l +瓊脂8g / l + ac2g / l培養基上的繼代效果最好,繼代時間最好在15d左右。The results showed that high concentration of 2, 4 - d was required for callus induction from mature seeds of tall fescue, and combination of 8mg / l 2, 4 - d with 2mg / l aba gave best induction effects. by slicing sterilized seeds longitudinally or cutting embryos, callus induction frequency was profoundly increased over intact seeds from one and half to eight times. adoption of ms basal medium and supplementation of 0. 5g / l casamino acids and 0. 5g / l glutamine in medium were found to help to facilitate callus induction
研究表明,高羊茅成熟種子愈傷組織誘導需要較高濃度的2 , 4 - d ,以8mg l2 , 4 - d與2mg laba配合能獲得最佳的誘導效果;種子滅菌后縱切或切胚,可使出愈率成倍提高;採用ms基本培養基和在培養基中添加0 . 5g l的水解酪蛋白與谷氨酰胺也有助於提高出愈率;低劑量( 10gy )射線輻照處理對成熟種子愈傷組織尤其是胚性愈傷組織形成有一定的刺激效應。Preliminary studies on tissue culture of pugionium cornutum
沙芥愈傷組織培養的初步研究Callus induction and darkening inhibition in tissue culture of taxus chinensis var. mairei and taxus media
兩種紅豆杉植物的愈傷組織培養及褐化抑制In contrast, there was very little difference in gene expression between calli subcultured for one week and two weeks with only 0. 78 % of the genes showing significant different expression. ( 4 ) the 14 genes with different expression level between the control and the transformed sublines a78 - 3 and a78 - 4 were classified by their function
88的未鑒定功能基因的表達呈顯著差異;而在愈傷組織培養二周與愈傷組織培養一周之間,則在第大類、第v大類和第大類未鑒定基因中各有1個表達呈顯著差異。分享友人