挑取菌落 的英文怎麼說
中文拼音 [tiāoqǔjūnlà]
挑取菌落
英文
picking colony-
Niger with phytase activity about 2250 u / ml which selected by protoplast - uv mutation was used as original, prepared it into fungu - suspend - liquid, through uv mutation, daub on the filter - substract. pre - primary - selection was according to the lucency - circle, primary - selection was one fungus inoculate one flask to ferment, secondary - selection was using several high phytase activity fungus through one fungus inoculate 2 - 3 flasks to ferment. then one or two high phytase activity fungus of the secondary selection were used in the next mutation cycle
首先用粗略制備的原生質體經紫外誘變篩選到一株酶活為2250u ml的實驗出發菌；制備成菌懸液，紫外燈下照射誘變，紅光下塗抹篩選平板，恆溫培養2 3d ；按透明圈大小進行預初篩，挑選徑圈比大的菌落接斜面，恆溫培養3d ；按一株一瓶的方式進行初篩；從中選取酶活較大的3 5株，按一株2 3瓶的方式進行復篩；挑取酶活大且穩定的1 2株進入下一代誘變篩選。Take a portion of the same colonies as under 3. 8. 1 by means of a wire and stab inoculate a glucose agar tube. incubate at 37 ? 1 c for 24 h
用接種針挑取與3 . 8 . 1實驗相同的菌落的一部分接種至葡萄糖瓊脂試管. 37 ? 1 c培養24小時。The asd mutants of salmonella thphimurium have an obligate requirement for diaminapimlic acid ( dap ) and will undergo lysis in environments deprived of dap
挑取單菌落質粒鑒定后，分別將其電擊經中間宿主x3730轉入最終宿主x4072 。A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge
用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接；用連接產物轉化大腸桿菌jml09感受態細胞，置含amp 、 x - gal和iptg的lb平板上培養12 20小時；挑取白色菌落於選擇性培養基擴大培養，堿裂解法小量提取質粒dna ，並進行酶切分析鑒定，結果獲得整合有prvge基因的重組質粒pugedna ，並與其它prv分離株進行ge基因序列同源性分析。Objective : to clone and sequence the cdna encoding metalloproteinase from the venom of agkistrodon acutus from northen mountain area of guangxi province. methods : one step method was used to extract total rna from the venom of agkistrodon acutus found in northern mountain area of guangxi province. different kinds of cdna encoding metalloproteinase were amplified by one step method ( rt - pcr and pcr reactions occurred in the same tube ) using different primers
方法：從桂北五步蛇毒腺中抽提總rna ，利用不同的引物，採用一步法（ rt - pcr和pcr在同一管內進行）擴增出不同的dna條帶，利用平端連接的方法將pcr擴增產物克隆至pgem - teasy載體，轉化大腸桿菌jm109 ，挑選白色菌落提取質粒，用pcr對其進行鑒定，直接利用純化pcr產物或提取陽性菌落質粒進行測序。In this study, the recombinant plasmid pmd - 18t - pea - h3 was cleavaged with ncoi, xhoi and inserted into the expression vector pet - 28c and subsequently subjected to restriction endonuclease analysis and sequencing, the result indicated that the prokaryotic expression vector pet - 28c - pea - h3 was constructed successfully. after the expression plasmid was extracted and transformed into expression hosts bl21 ( de3 ) of e. coli, the transformed hosts were induced by iptg, bysds - page and elisa analysis of host protein. the expression of the objective gene was detected, and it could account for 16. 28 % of the total host protein. inclusion body was prepared from the incubating expression hosts induced by iptg
同時將原核表達載體pet - 28c用nco ， xho雙酶切，回收酶切產物，將回收的酶切產物pea ， h3 ，載體進行連接，並轉入dh5感受態細胞內，培養12 - 18小時后，挑取陽性菌落，經nco ， xho雙酶切分析及pcr檢測，篩選到陽性克隆，其質粒測序結果表明成功地構建了毒性基因缺失的pea與人組蛋白h3融合基因的原核表達載體。分享友人