挑取 的英文怎麼說

中文拼音 [tiāoqǔ]
挑取 英文
picking
  • 挑 : 挑Ⅰ動詞1 （用肩膀支起扁擔來搬運） tote with a carrying pole; shoulder 2 （挑選） choose; pick; se...
  • 取 : Ⅰ動詞1 （拿到身邊） take; get; fetch 2 （得到; 招致） aim at; seek 3 （採取; 選取） adopt; assume...
  1. Screening target ' gene of artemisinin antimalarials using drug - western from cdna expressing library : 12 b - deoxoartemisinyl - ( 4 ' - oxyacetic acid ) phenyl ether was linked to bsa by using of edc cross ! inker and the product acted as drug - probe

    對重組pmd一18一t克隆載體及pqe一30表達載體雙酶切，提取tctp基因和pqe一30空載體並使二者重組，然後轉化m15 ，挑取陽
  2. Niger with phytase activity about 2250 u / ml which selected by protoplast - uv mutation was used as original, prepared it into fungu - suspend - liquid, through uv mutation, daub on the filter - substract. pre - primary - selection was according to the lucency - circle, primary - selection was one fungus inoculate one flask to ferment, secondary - selection was using several high phytase activity fungus through one fungus inoculate 2 - 3 flasks to ferment. then one or two high phytase activity fungus of the secondary selection were used in the next mutation cycle

    首先用粗略制備的原生質體經紫外誘變篩選到一株酶活為2250u ml的實驗出發菌；制備成菌懸液，紫外燈下照射誘變，紅光下塗抹篩選平板，恆溫培養2 3d ；按透明圈大小進行預初篩，挑選徑圈比大的菌落接斜面，恆溫培養3d ；按一株一瓶的方式進行初篩；從中選取酶活較大的3 5株，按一株2 3瓶的方式進行復篩；挑取酶活大且穩定的1 2株進入下一代誘變篩選。
  3. Take a portion of the same colonies as under 3. 8. 1 by means of a wire and stab inoculate a glucose agar tube. incubate at 37 ? 1 c for 24 h

    用接種針挑取與3 . 8 . 1實驗相同的菌落的一部分接種至葡萄糖瓊脂試管. 37 ? 1 c培養24小時。
  4. Shaking flask experiments and hplc analyses showed that 99 isolates still produced four components of avermectins b, in which the yields of avermectins b in 82 isolates were only about 0. 1 ~ 2 % of those produced by the parental strain olm73 - 12. 2 of 82 isolates were confirmed to be the correct gene replacement mutants by pcr and sequence analysis. the mutant only producing avermectins bl was not detected

    搖瓶發酵和hplc分析結果表明，有99個突變株仍然產阿維菌素b的4個組分，其中82株的發酵單位很低，僅為出發菌株olm73 - 12的0 . 1 2 ，從中挑取2株經pcr擴增和測序驗證，均發生了正確的基因取代；沒有檢測到僅產b1的突變株，這表明阿維菌素b2組分的產生並不是因為阿維菌素pks上dh2的部分活性所造成。
  5. 3. the cold - heat method without the snailase and zymolyase was used as the pcr direct selecting of colony. to select the colony and suspend with 10 n 1 ddw, and then incubate

    改進了重織酵母囪落的pcr鑒定方法：冷熱處理法，即挑取單克隆於10ul水中懇浮， m及5分鐘、液氮處理2分鐘， 9了c15分鐘，然後進行kr擴增。
  6. At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged

    在本實驗中，利用隨機12肽庫對抗豬瘟病毒（ classicalswinefeverviruscsfv ）糖蛋白me2的單抗a11進行表位篩選，經過四輪篩選以後，隨機挑取11個克隆作競爭- elisa檢測，結果表明，所挑11個克隆中，有9個克隆能對me2蛋白和a11反應產生抑制作用，抑制率最高可達64 ； dna測序以後經過dnastar軟體分析，發現它們的核心序列為anwralsl ，該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別；人工合成含核心序列的多肽經間接elisa試驗證實，也能被a11識別。
  7. 10 clones resistant to spectinomycin were obtained, 3 resistant transformants were subjected to three rounds of spc selection

    其中挑取3個抗性轉化子進行三輪的抗生素同質化篩選。
  8. But of their children, who were left after them in the land, whom the children of israel consumed not, them did solomon make to pay tribute until this day

    8就是以色列人未曾滅絕的，所羅門挑取他們的後裔作服苦的奴僕，直到今日。
  9. The asd mutants of salmonella thphimurium have an obligate requirement for diaminapimlic acid ( dap ) and will undergo lysis in environments deprived of dap

    挑取單菌落質粒鑒定后，分別將其電擊經中間宿主x3730轉入最終宿主x4072 。
  10. Here is the account of the forced labor king solomon conscripted to build the lord ' s temple, his own palace, the supporting terraces, the wall of jerusalem, and hazor, megiddo and gezer

    15所羅門王挑取服苦的人、是為建造耶和華的殿、自己的宮、米羅、耶路撒冷的城墻、夏瑣、米吉多、並基色。
  11. And this is the reason of the levy which king solomon raised ; for to build the house of the lord, and his own house, and millo, and the wall of jerusalem, and hazor, and megiddo, and gezer

    王上9 : 15所羅門王挑取服苦的人、是為建造耶和華的殿、自己的宮、米羅、耶路撒冷的城墻、夏瑣、米吉多、並基色。
  12. And this is the reason of the levy which king solomon raised ； for to build the house of the lord ， and his own house ， and millo ， and the wall of jerusalem ， and hazor ， and megiddo ， and gezer

    15所羅門王挑取服苦的人，是為建造耶和華的殿，自己的宮，米羅，耶路撒冷的城墻，夏瑣，米吉多，並基色。
  13. The amplified phage be used repeat the selection. the selection was repeat three times. the titter of phage indicated that the eluted phage enriched with the rounds increasing

    山西醫科大學碩士學位論文第三次淘洗的噬菌體感染大腸桿菌xli一blue ，鋪制平板，隨機挑取18個分離良好的噬斑，制備噬菌體原種。
  14. Cells were then harvested by centrifugation and the pellet was resuspended in phosphate - buffered saline ( pbs ) containing 5 mm edta. after sonication, debris was removed by centrifugation at 10000 x g for 15 min at 4

    挑取轉化后的大腸桿菌提取質粒， ecori和hindln酶切質粒進行鑒定，瓊脂搪電泳顯示含有大約800kb的目的片段。
  15. A pair of primers were designed and synthesized based on the published ge gene sequence of prv - rice strain for amplifying ge gene of prv min - a, yielding a 1. 7kb band. the segment was linked to puc19 plasma dna by means of t4 dna ligase, transformed into e. coli jm109 permissive cells, and incubated on lb fray containg amp, x - gal and iptg. small amount of plasma was extracted by base cleavaging for enzyme digest analysis and pcr, resulting in recombinant plasma puge dna containing prv ge

    用t _ 4dna連接酶使ge基因與經bamhi 、 kpni同樣雙酶切的puc19質粒dna連接；用連接產物轉化大腸桿菌jml09感受態細胞，置含amp 、 x - gal和iptg的lb平板上培養12 20小時；挑取白色菌落於選擇性培養基擴大培養，堿裂解法小量提取質粒dna ，並進行酶切分析鑒定，結果獲得整合有prvge基因的重組質粒pugedna ，並與其它prv分離株進行ge基因序列同源性分析。
  16. To prepare the wild type mbl in prokaryotic system, a pair of primers was designed and synthesized, and was used to amplify mbl gene from the recombinant vector pgem - mbl that contans wild type mbl cdna. a recombinant prokaryotic expression vector, pet28 - mbl, was constructed by inserted the mbl gene into plasmid pet28 ( b ), and after transfected it into ecoli bl21 ( de3 ) and induced with iptg, recombinant mbl protein was expressed successfully

    本實驗另選用了原核表達質粒pet28 （ b ） ，根據已構建好的含有mbl野生型基因的t載體pgem - mbl ，設計一對引物， pcr擴增mbl基因，凝膠回收，雙酶切pcr產物和pet28 （ b ）質粒， t4連接酶連接，轉化大腸桿菌dhsa ，氨芋選擇培養挑取克隆鑒定。
  17. Correlation analysis was done using the % vol of matched spots from the two group, which is a useful function offered by the software. consequently, the correlation coefficients of the plc - 1 + / + group were 0. 858, 0. 867, 0. 875, yet the other were 0. 842, 0. 846, 0. 863. we cut out 7 bad - matched spots ( exist in one group and out of the other ) after analysis by auto matching and manual correction, three of which has been analyzed by pmf up to now. of the three analyzed, one has been idendified as vimentin definitely, while the other two can not be identified

    經過軟體分析和手工修正，挑取了7個在兩組凝膠中不能匹配的蛋白質斑點（一組存在而另一組消失） ，目前已對其中3個進行了質譜鑒定，其中一個確切鑒定為vimentin ，而另外兩個不能得到鑒定。 vimentin是一種第三類中間纖維，與c - fos 、 c - jun和creb具有較高的同源性，在細胞分裂時常常大量地重新組織，發生顯著的磷酸化。
  18. The fragments digested with restriction endonucleases ecor i and hind iii were subsequently cloned into the vector pkk223. 3. e. coli jm 109 transformed with the expression plasmid was grown in m9 media to an optical density of 0. 7

    把重組質粒轉化入大腸桿菌jm109中，挑取轉化成功的單克隆菌，接種到含amp的lb培養基中，於37振搖過夜。
  19. After the primary and secondary selection, 7 strains were tested to have higher productivity than the starting strain. of the 7 strains, strains s1 - 007, s1 - 123 and s1 - 018 were proved to produce more maduramicin than the starting strain. compared with 6236ug / ml of the starting strain, the productivity of the above three strains reached 8020 g / ml, 8254 g / mland9175 g / ml respectively

    出發菌株尤馬馬杜拉放線菌s - 1 ，經過鏈黴素抗性篩選，挑取223個菌株，經過初篩和復篩得到7株菌株馬杜黴素的產量高於出發菌株，其中3個菌株s1 - 007 、 s1 - 123和s1 - 018的產素量與出發菌株的6236ug ml相比有較大提高，分別為8020ug ml ， 8254ug ml和9175ug / ml ，尤其是突變菌株s1 - 018的產量比出發菌株高47 。
  20. Objective the origin and the nature of the hodgkin and reed - sternberg ( h / rs ) cell of hodgkin ' s lymphoma ( hl ) has been attracting a lot of medical researchers engaged in studying it, for its character by scattered large atypical cells residing in a complex admixture of inflammatory cells. great improvement has been made since a new method of isolation of single h / rs cells from a frozen section had been set up by kuppers in 1994, and many studies have approved of the b - cell derivation of h / rs cell. lt has been reported that h / r - s cells might partly be originated from b - cell in our research before, but at the same time, we also found that only 18. 8 % of h / rs cells express cd20, 31. 3 % of immunoglobulin heavy chain ( igh ) rearrangement have been revealed

    目的霍奇金淋巴瘤（ hodgkin ' slymphoma ， hl ）由於它的惡性腫瘤細胞? h rs細胞一般只佔腫瘤組織的極少部分（不到1 ） ，且散在分佈在背景細胞間，因此對于h rs細胞的來源和性質研究一直是人們探索的目標。 1994年德國科隆大學k ppers等發展了一種從冰凍組織切片上用顯微操作儀挑取單個h rs細胞的顯微切割方法進行h rs細胞基因分析后，人們對h rs細胞來源的研究有了突破性的進展，多數支持b細胞來源。我們的前期研究也支持部分h rs細胞為b細胞來源，但我們發現只有18 . 8的h rs細胞表達cd20 ， 31 . 3的hl檢測到igh基因重排克隆性條帶。
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