接合蛋白質 的英文怎麼說

中文拼音 [jiēgědànbáizhí]
接合蛋白質 英文
conjugated proteins
  • 接 : Ⅰ動詞1 （靠近;接觸） come into contact with; come close to 2 （連接; 使連接） connect; join; put ...
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 質 : Ⅰ名詞1 （性質; 本質） nature; character; essence 2 （質量） quality 3 （物質） matter; substance;...
  • 接合 : joint; zygosis; juncture; articulation; concrescent; nexus; coaptation; syndesis; synapsis; meet;...
  • 蛋白質 : [生物化學] protein; proteide （舊稱「朊」, 由多種氨基酸結合而成的高分子化合物）
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. According to medical research, when vitamin b12 enters the body, it forms a compound with an intrinsic factor secreted by the parietal cells large cells of the peptic glands on the gastric mucosa mucous membrane of the stomach, before being absorbed by receptors in the ileum lower part of the small intestine in the presence of calcium ions

    在維他命b12的吸收方面，根據醫學報導，維他命b12在吃進人體后，首先會在胃部，和胃壁細胞parietal cells分泌的一種蛋白質內因子intrinsic factor結合，形成復合物后，再由小腸段中的回腸ileum吸收。在回腸之接受體receptors吸收時，需要有鈣離子之存在。
  3. During the different time point, we retrieve the cell supernatant and through the electrophoretic analysis and immunoblot of the recombinant protein indicated that the cell which transfected the recombinant eukaryotic gene expressing vector could synthesis and excrete the peptide effectively

    提取培養細胞總rna反轉錄cdna測序結果表明人生長激素基因組dna在家蠶bmn細胞內能正確轉錄，剪接。蛋白質電泳分析和免疫學檢測證明轉染細胞能夠有效合成並分泌hgh蛋白質。
  4. Many glycoproteins of lower and higher eucaryotes are attached to the plasma membrane by means of a glycosylphosphatidylinositol ( gpi ). gpi - anchored proteins are synthesized on membrane - bound ribosomes. upon translocation of the pro - protein across the endoplasmic reticulum membrane, gpi : protein transamidase ( gp1t ) recognize and removes the carboxy terminal gpi signal sequence and attaches a gpi molecule to the newly exposed carboxy terminal amino acid

    Gpi化前體蛋白在依附於膜的核糖體上合成，當其易位穿過內質網（ er ）膜后，被gpi ：蛋白質轉酰胺基酶（ gpit ）識別， gpit在移走其羧基端gpi信號序列的同時將gpi分子連接至新生成的氨基酸位點上。
  5. Rna showed a positive pyronin reaction in follicle cells after vitellogenesis beginning. lt indicated that follicle cells perhaps takcd part in the synthesis of yolk precursor

    穩定性； rna的量在卵黃生成準備階段則有所增多，證明其與蛋白質卵黃的合成直接相關。
  6. Mass spectrometry of synthetic hw - ma and rgd - hw are in full agreement with those speculated theoretically, which proves the success of peptide synthesis and refold. on isolated mouse phrenic nerve - diaphragm preparations, hw - ma can block the neuromuscular transmission in 35 minutes or so ( l 10 - 5 g / ml ), its biological activity shows 73 % decrease comparing with biological activity of native hwtx - i. it proves t hat the protein engineering of synthetic chimera hwtx - i has gained success to some extent, although it did not achieve our expectations. thus it proved that hwtx - i can be using as natural scaffold for protein engineering. and also emphasized the importance of " local stereo circumstances " of activity site when the foreign activity site was transferred into a natural scaffold

    濃度為1 / 1059 / ml的hw一ma突變體能可逆阻斷小白鼠隔神經書高肌的接頭傳遞，阻斷時一間為35min左右，與天然hwtx一i比較，生物學活性下降3一4倍，說明合成的突變體改造獲得了一定的成功，盡管與我們預期的目標有一定的差距，從而證明hwtx一i可以作為蛋白質工程研究的天然分子骨架，同時也強調了往天然分子骨架中轉移外源活性位點時維持活性位點「局部立體環境」的重要性。
  7. In order to check if it is the aim gene, we devised pcr with a new pair of primer, sequenced and registered the product with registration number : af449446. moreover we forecast and analysis the primary, secondary, tertiary and quaternary structure of the three protein : osftszi, crftszi and crftsz2 which has already cloned by our team before. after that we construct ftsz molecular evolution tree to site them in

    又將生物信息學技術同實驗技術相結合，針對ftsz保守區設計引物擴增出一條衣藻ftsz片段，進行est搜索、比對、拼接，最終克隆出新基因crftsz1 ；連同本試驗室曾經獲得的另一個衣藻crftsz2基因進行蛋白質的一、二、三、四級結構預測、分析及比較尋找進化線索，建立了ftsz蛋白的氨基酸進化樹作進一步的進化定位。
  8. The applicator on the stick, a strip of synthetic proteins, then detects hiv antibodies in blood in 20 minutes or less

    棒上的塗藥器是一條狀的人工合成的蛋白質，接著在血液中發現hiv抗體大約需要20分鐘。
  9. The main concept of this work includes the following aspects : ( 1 ) based on the soft - docking algorithm which is improved upon wodak and janin ' s protein - protein rigid docking algorithm, we took seventeen protein - protein complexes for examples to study the method how to select the near - native structure from docking conformations

    本論文的主要研究內容如下： 1採用本組在wodak和janin的剛性對接演算法基礎上改進的半柔性對接演算法，以17種蛋白質-蛋白質復合物體系為例，我們研究了如何正確有效地從對接采樣中挑選出近天然構象的方法。
  10. Further experiments revealed that as the healing nerve began to form the protein myelin - the insulating sheath around nerves - this receptor not only caused a reduction in the macrophages ' binding to myelin, but also an outright repulsion from the forming myelin

    深入研究揭示，當恢復的神經開始產生蛋白質髓鞘-圍繞神經的絕緣鞘-這種受體不但抑制巨噬細胞與髓鞘的結合，還會直接抑制髓鞘的形成。
  11. A novel aqueous two - phase system can be formed by the mixtures of a polymer and cationicanionic surfactants. such a system can be used as a partitioning system of proteins. in this work, we investigated the formation, phase behavior and protein partitioning in aqueous two - phase systems formed by dodecyltriethylammonium bromide / sodium dodecylsulfate / peg and dodecyltriethylammonium bromide / sodium dodecylsulfate / dextran. the ligands with affinity were attached to the polymers and the affinity partitioning of proteins was investigated. it was shown that the surfactants and polymers are enriched in different phases of aqueous two - phase systems. phase separation are promoted by increasing temperature and adding inorganic salts. different proteins are partitioned in different phases. the selectivity of protein partitioning is increased by adding ligands with affinity

    報道了由正負離子表面活性劑與高聚物混合溶液形成的一種可用於蛋白質的分離及分析的新型雙水相萃取體系.研究了正負離子表面活性劑（溴化十二烷基三乙銨/十二烷基硫酸鈉）分別與葡聚糖和聚乙二醇混合雙水相體系的形成規律、相行為及牛血清蛋白和溶菌酶在雙水相體系中的分配.通過在高聚物分子中接上親和配基，研究蛋白質在雙水相體系中的親和分配.結果表明，在該體系中，表面活性劑與高聚物分別富集於不同相中.升高溫度及加入無機鹽均可促進雙水相體系的形成，不同蛋白質可分配于不同的相中.親和配基的引入極大地增強了蛋白質分配的選擇性
  12. The polymers are also able to protect the protein from proteases in the upper small intestine and can temporarily open the connections between intestinal cells, allowing the protein to pass through

    在小腸前段，這類聚合物還能保護蛋白質免遭蛋白酶的破壞，並且可以暫時拓寬腸細胞間的連接，好讓蛋白質穿過。
  13. Glycosylation - - the addition of carbohydrates to proteins

    醣化作用- -在蛋白質上連接碳水化合物。
  14. Lymphotoxin ( lt ) is a kind of pleiotropic lymphocyte - secreted cytokine which mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. in order to increase the antitumor activity of lymphotoxin and reduce its side effects, the recombinant plasmid pet36b - lt 27 was constructed to express soluble fusion protein cbd - lt 27. the active form of lt 27 could be collected directly with several simple steps by three kind of components on the expressed fusion protein

    本研究通過構建表達n端缺失27個氨基酸的淋巴毒素融合蛋白的重組質粒，在大腸桿菌中實現融合蛋白的可溶及分泌表達，同時利用表達載體上的幾種特殊序列經簡單的分離純化步驟直接獲得大量的有生物活性的淋巴毒素缺失體lt 27 ，為尋找一種高抗腫瘤活性、低臨床毒副作用的生物抗癌藥物進行了有效的探索。
  15. Through numerous experiment and analysis of scattering characteristic about fat and protein in milk, we find their characteristic about fat and protein in milk, we find their scattering law and establish the correlative scattering model of fat and protein in milk. especially, we adopt surface fitting to break through this problem from theory and technology, without isolating protein from milk, we can directly measure the protein in milk, that is to say, completely realizing the quickly accurate measurement of protein in milk. furthermore, we bring prosperity for the field of measuring multi - ingredient in milk through light scattering theory

    經過大量實驗和對牛乳脂肪蛋白質的散射特性分析，我們找到了他們的散射規律，並建立了牛乳脂肪和蛋白質的關聯散射模型，並採用多元線性回歸的曲面擬合法徹底突破了這一難關，無需把蛋白質從牛乳中分離出來，可直接對牛乳這一復合體中的蛋白質進行測量，完全實現了快速而準確的牛乳蛋白質測試，為利用光散射原理同時測量牛乳多成份這一研究領域開辟了光明而廣闊的前途。
  16. A newly made amino acid chain folds into a distinctive shape depending on the positioning of hydrophobic amino acids, which like to cluster together away from the cell ' s watery cytoplasm, leaving hydrophiles to form the protein ' s surface

    剛合成好的胺基酸長鏈，會根據疏水性胺基酸的分佈位置，摺疊成蛋白質的獨特形狀：疏水性胺基酸彼此向內聚攏，避免接觸到富含水的細胞質環境；親水性胺基酸則向外翻出，構成蛋白質的表面。
  17. Combining the research group ' s works, in terms of the international and national progress of protein - protein docking approaches, the detailed review was made about the four stages mentioned above

    結合國內外研究蛋白質蛋白質分子對接方法進展和本研究小組的工作，對以上四個環節做了詳細的綜述。
  18. Generally, the protein - protein docking procedure is composed of four stages : searching of the binding modes of the receptor and the ligand, filtering of docked modes to eliminate the irrational docked structures, optimizing the structures, evaluating the docked modes with the refined scoring function and ranking them to obtain the near - native structures

    通常，蛋白質蛋白質分子對接包括四個階段：搜索受體與配體分子間的結合模式，過濾對接結構以排除不合理的結合模式，優化結構，用精細的打分函數評價、排序對接模式並挑選近天然構象。
  19. Our goal is to establish a simple and effective empirical approach to calculate the conformational entropy and the binding free energy by analysis of j : protein interface. three variables of the binding interface information of 20 protein complexes, including the binding accessible number ( nb ), hydrophilic pair ( n ^, ) and apolar solvent - accessible surface areas ( & asaapol ) are analyzed

    本論文在此分析了20組蛋白質二聚體分子的結合界面三個有效的參數信息，即界面殘基側鏈可接觸數（ n _ b ） 、親水對數（ n _ （ pair ） ）和非極性溶劑可接近表面積的變化（ asa _ （ apol ） ） 。
  20. Professor lefkowitz is awarded the shaw prize in life science and medicine for his relentless elucidation of the major receptor system that mediates the response of cells and organs to drugs and hormones

    尼科威教授獲頒邵逸夫生命科學與醫學獎以表彰他多年來對gpcr （ g -蛋白質偶合接受體）的關鍵性的貢獻。
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