接合誘導 的英文怎麼說

中文拼音 [jiēgěyòudǎo]
接合誘導 英文
cross induction
  • 接 : Ⅰ動詞1 （靠近;接觸） come into contact with; come close to 2 （連接; 使連接） connect; join; put ...
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 誘 : 動詞1. （誘導） guide; lead; induce 2. （使用手段引人隨從自己的意願） lure; seduce; entice
  • 導 : 動詞1. （引導） lead; guide 2. （傳導） transmit; conduct 3. （開導） instruct; teach; give guidance to
  • 接合 : joint; zygosis; juncture; articulation; concrescent; nexus; coaptation; syndesis; synapsis; meet;...
  • 誘導 : guide; lead; induce; guidance; induction
  1. Subconfluent cultures of rat were maintained in dmem / 10 % fbs, 24hrs before neuronal induction, media were replaced with pre - induction media consisting of dmem / 10 % fbs / lmm beta - mercaptoethanol ( bme ), to initiate neuronal differentiation, the preinduction media were removed, and the cells were washed with pbs and transferred to neuronal induction media composed of dmem / serum - free media, 5hrs later, 40ul dmso was given to every hole containing 2ml each

    分別取第5代和第13代的mscs ，以8x10 cm 『濃度接種於六孔板中的蓋玻片上，制備細胞爬片，每孔加zinl培養液。達到80融合時，更換新鮮培養液，並在培養液中加人終濃度為lrnm的p一流基乙醇，誘導24小時， pbs洗滌，而後換成無血清的培養液。
  2. The global regulator csra of e. coli is a specific mrna - binding protein. csra negatively regulates several metabolic pathways that are induced post - exponentially, including glycogen biosynthesis, gluconeogenesis, and glycogen catabolism ; positively controls gene expression within the glycolytic pathway ; and also csra modulates the levels of enzymes that participate directly in pep metabolism

    Csra是整體調控網路的調控基因，可負調控指數生長後期誘導的一些代謝途徑，包括糖原的生物合成、糖原的分解代謝、糖異生，而對糖酵解的一些重要基因的表達則執行正調控功能， csra也調控直接參與pep代謝的三個酶的活性水平。
  3. But without rdrp transcription were tested. so it is suggested that tm - 22 is a relative to multi - protein transcriptional coactivator. mip204 and pr - la gene expression can be induced in resistant response and do not relate to rdrp gene transcription

    因此，可以認為tm一22基因的編碼蛋白與復合蛋白轉錄因子有關，在抗病反應中能夠誘導五刃滬204和屍尺一ia基因的表達，與rdrp基因的轉錄沒有直接的聯系。
  4. This time, using cdna of zmcdc5 as template, we amplify a sequence by means of pcr technology. and then, using restrict endoenzyme and ligase, we conjunct the 0. 8kb length dna sequence in a expression vector, pet - 30a. after induction, expression and purification, we obtained a 35. 4kda truncated fusing zmcdc5 protein which contains 267aa ( 647 to 914aa in zmcdc5 ). with the purified protein, we got its antibody and testified the antibody by means of western blotting and dot blotting

    本實驗是以zmcdc5的cdna為模板，使用pcr獲得基因片段，再通過酶切連接，將得到的0 . 8kb的基因片段構建於pet - 30a表達載體上，經過誘導表達和純化，獲得zmcdc5的融合蛋白，其中包括了zmcdc5925個氨基酸中647 914共267個氨基酸殘基
  5. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  6. Based on the structure and function analysis of hirudin, a potent thrombin inhibitor, and some platelet aggregation inhibitors, which contain the recognition sequence argglyasp as their functional motif, two chimeric antithrombotic molecules were designed by introducing rgd sequence to hirudin cterminus. these chimera genes were constructed by pcr and inserted into the expression vector pet21a, the constructs were confirmed by restriction enzyme digestion and dna sequence analysis. these recombinant plasmids were transformed into

    經限制酶消化和dna序列分析，證明兩種重組質粒與設計完全一致。由於rgd -水蛭素嵌合基因上游連接了金黃色葡萄球菌蛋白a spa的信號肽序列，在iptg誘導下兩種嵌合分子都獲得了分泌表達，表達產物主要集中在細胞周質空間。
  7. Above all, this paper discusses the frame, system functions, user demands, construction preconditions and conformity planning based on the introduction of study and application situation ; then, planning project, planning process and foundation of guidance system in grades, including guidance strategy in four grades are studied ; whereafter, the thesis analyzes setting requirements and modes, installation angle, dimension and colour, display contents and arranging sequence and fonts of parking guidance sign, it is mainly studied that calculational method of distance between the variable message signs

    本論文首先在介紹停車誘導系統研究與應用情況的基礎之上，論述系統框架結構、系統功能、用戶需求、建設停車誘導系統的條件與停車誘導系統與智能交通系統的整合規劃等問題;接下來制定了停車誘導系統規劃方案，提出區域性停車誘導系統規劃步驟，研究分級誘導體系的建立方法，提出四級誘導策略;然後分析停車誘導標志布設的具體要求、設置方式、安裝角度、尺寸與顏色、顯示內容及排列順序、字體等問題，重點研究並給出可變信息板設置間距的計算方法。
  8. According to the differential geometry theory, the principle curvatures and principle relative curvature of the spur gear and the fg in line contact and point contact, the tooth surface relative speed and the tooth contact interface are analyzed, whose distributing on the tooth surface are visualized by computer

    在齒面的幾何特性研究中，利用微分幾何的原理分析了圓柱齒輪和面齒輪的齒面曲率、線接觸和點接觸面齒輪傳動的主誘導法曲率以及嚙合過程中齒面的相對速度和接觸區域計算問題，並獲得有益的結論。
  9. Equations of mesh, shorting contact line, undercutting limit line, meshing limit lines and the existence conditions, angle between the direction of relative speed and the direction of contact line, induced normal curvature about every point on the contact line are established. moreover, the paper also theoretically analyzed the error of the grinded gear surface. on the basis of the theory, the computer program is worked out to automatically produce the contact line and the boundary curves of mesh. analysis of meshing circs under different parameters can be done so that we can gain the best process condition

    首先對漸開面二次包絡理論進行了深入的探討，推導出了兩次嚙合的嚙合方程式、瞬時接觸線方程式、根切界限線方程式、嚙合界限線的方程式及其存在條件，相對運動速度方向與接觸線方向的夾角及接觸線上各點的誘導法曲率；此外，還對磨齒后工件的齒面誤差進行了理論分析；並在理論基礎上編制了相應的計算機程序，自動生成接觸線族及嚙合界限線，對不同參數條件下的嚙合情況進行分析，可以使工藝條件達到最佳狀態；最後研究了磨齒裝置，設計了磨齒機的傳動系統。
  10. Tooth surface equations and contact line equations of the involute worm gearing are established according to its machining methodology. meshing characteristics in rigidity condition are studied. these characters include the inductive normal curvature along the contact line on the worm surface, the first and the second limit lines during the engage of the worm gear, and the influence of the intersect angle between the contact line the relative velocity of the worm and the gear on transmission performance

    根據漸開線圓柱蝸桿傳動的創成方法，建立了漸開線圓柱蝸桿和蝸輪的接觸線方程式、齒面方程式，分析了漸開線圓柱蝸桿傳動的剛性嚙合特性，如蝸桿與蝸輪嚙合時的誘導法曲率、一類界限曲線、二類界限曲線和蝸桿與蝸輪間相對速度方向和接觸線方向的夾角對傳動性能的影響； 2
  11. P hage induction following conjugation of a lysogenic bacterium with a nonlysogenic one

    伴隨著溶源菌與非溶源菌接合的噬菌體誘導。
  12. Dcs play a pivotal role in the development of antitumor immunity. t lymphocytes immune response induced by direct antigen - pulsed dcs can not maintain longer for peptides - mhc department or mhc molecules degeneration. the method of dcs loading with tumor antigen gene is another potential approach to enhancing and maintaining immune response

    在用抗原直接刺激dc時，由於抗原- mhc復合物解離或細胞mhc分子降解而不能維持所誘導的t細胞免疫應答，藉助載體把腫瘤抗原基因導入dc后腫瘤抗原在dc內的持續表達能增強其抗原提呈能力並能維持所誘導的免疫效應。
  13. Objectives to improve the effect of a single mtb8. 4 dna vaccine, we constructed a chimeric mtb8. 4 / hil - 12 eukaryotic plasmid by linkage of mycobacterium tuberculosis mtb8. 4 gene to human il12 gene with a simple linker ( gly4 - ser ) 3. we analyzed the immunogenicity of chimeric dna vaccine and investigated the immune responses elicited when mtb8. 4 / hil12 was presented as endogenous ag

    目的：以il - 12作為分子佐劑，與結核桿菌新抗原mtb8 . 4基因連接形成嵌合分子，將其克隆到真核表達質粒中，構建成嵌合dna疫苗，研究其在小鼠體內誘導細胞免疫應答的效果及對c57bl 6n小鼠的免疫保護作用，為尋求安全、有效、廉價的結核病新疫苗打下基礎。
  14. The article brings forth a reasonable traffic consuming module to coordinate the game relationship and improve road network efficiency, that is, the optimized or almost optimized status of the system and customer - optimized status with traffic information lacking could come into fact when the traffic governor allot traffic flow based on system equilibrium principle, take passenger ’ s path choosing behavior into consideration and take the preponderant advantages to make the traffic flow at optimized or almost optimized status through traffic control system and guidance system

    本文提出了一種有效協調二者關系，建立合理的交通消費模式，以提高路網效率的解決方案:當交通管理者以系統最優的方法進行交通流分配時，應預先考慮用戶的路徑選擇行為，利用其信息優勢和主導地位通過交通控制和交通誘導使交通流接近或達到系統最優狀態，同時實現出行者信息缺乏狀態下的用戶最優。
  15. Then using ecbp21 antibody and immunogold transmission electron microscopy method, we studied the subcellular localization of ecbp21. the results indicated that the gold particles were mainly localized in the cell wall in callus cells and rachis cells of angelica dahurica. these results indicated that ecbp21 mainly localized in cell wall, which provide a direct evidence of the extracellular existence of ecbp21. furthermore, using ecbp21 antibody and immunohistochemical method, we studied the organic specially distribution of ecbp21, the results indicated that ecbp21 distributed in all organize, but it distributed more in leave n flower rachis than in leafstalk and root

    首先，構建了ecbp21表達載體，誘導了重組蛋白的表達，並通過膠回收法獲得了大量純化重組ecbp21蛋白，制備了高效價、高特異性抗體；隨后，利用ecbp21抗體，結合免疫膠體金電鏡定位技術進行了ecbp21亞細胞定位研究，結果顯示：在白芷愈傷組織細胞和花序軸細胞中金顆粒主要分佈在細胞壁區域，而在細胞內未發現或僅有少量金顆粒分佈，表明ecbp21蛋白主要定位於細胞壁區域，這為細胞外cambp （ ecbp21 ）的胞外存在提供了直接證據：進一步，利用ecbp21抗體，通過免疫組織化學分析研究了ecbp21組織特異性分佈狀況，結果表明ecbp21在白芷各組織中均有分佈，但在葉、花、花序軸中分佈較多，而在葉柄、根中分佈較少。
  16. Considering the water spraying can effectively increase the contact area between gas and water and promote the hydrate production rate, a batch reactor with water spraying was built in order to experimentally investigate the forming performance of natural gas hydrate, the system ' s state parameters ' variation and its effect on induced time of initial pressure and water temperature

    摘要考慮到水的霧化可以有效提高氣水接觸面積，有助於提高水合物生產速率，設計和建造了一個半間歇式霧流強化水合物實驗裝置，用於探索和揭示噴霧強化天然氣水合物制備過程的基本特性，包括制備水合物的形態特徵，形成過程中參數的變化規律，以及系統初始壓力和初始水溫度對形成過程誘導時間的影響。
  17. The optimized result of cell immobilization cultures was acquired, including the support matrices pretreatment, the class and size of support matrices, the level of dissolved 62 and inoculum. the optimal ratio of hormones was got by uniform design according to its high immobilization level, high metabolism and retaining vigorous cells with long period. a medium which fit well all the conditions was obtained, and the efficiency of producing ginkgolides by cell culture improved obviously

    進行了優良種系的誘導和優選，得到了一種生長快、分散性好，並很好的符合固定化要求的細胞株系；對固定化條件進行了最優化配置- -從載體預處理、載體種類、載體量、大小和溶氧到細胞接種量系統的研究；採用均勻設計尋求一種高固定化、高代謝及長期保持細胞活力的激素配比，最終得到一種基本滿足上述要求的培養基。
  18. In this study, pichia pastoris system had been utilized for expression of fmdv 2c3abc gene which aimed for establishing a sensitive and specific molecular dignosis method. first, 2c and 3abc genes were amplified individually from p2 and 3abc postive clones and ligated together using pcr method, then this 2c3abc product was cloned into pgem - t easy vector and transformed e. coli dh5a competent cell. a postive recombinant plasmid which contained whole 2c3abc gene had been confirmed by pcr, enzyme digestion and sequencing. after that, the 2c3abc gene was sub - cloned into ppiczaa expression vector and transformed e. coli dh5 a competent cell and selected by zeocin ? antibiotic. the postive recombinant expression vector was linerized and electro - transformed pichia pastoris smd1168 competent cell. a recombinant pichia pastoris had been obtained by zeocin ? antibiotics selection and induced with 0. 5 % methanol for target protein expression. the expression product was analysised by sds - page and western blotting assay. the result sh owed that 2c3abc gene was expressed successfully in pichia pastoris and the product was a 95ku fusion protein which could be recognized by anti - fmdv serum. the amount of target protein was over 15 % of the total bacteria protein by gel thin layer scanning analysis. this research had supplied materials for establishing a fmd diagnosis method to differentiate infected animals from vaccinated animals

    首先，用p2和3abc陽性克隆通過連接pcr方法獲得目的基因並將其克隆到pgem - teasy載體上，並轉化e colidh5a感受態細胞中，經pcr 、酶切以及測序證明得到了完整的2c3abc基因，並與國內外參考序列進行比較分析。然後，將目的基因亞克隆于ppiczaa表達載體並轉化大腸桿菌dh5a ，以zeocin ~ （ tm ）抗性篩選陽性克隆，大量提取重組表達質粒並用pme酶線性化后電轉化入畢赤酵母smd1168感受態細胞，通過zeocin ~ （ tm ）抗生素梯度濃度篩選，獲得重組酵母用0 . 5甲醇誘導表達，通過sds - page電泳、 westernblotting分析，結果表明， 2c3abc基因在畢赤酵母中成功表達，其表達產物為一95ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。
  19. From the result of electrophoresis, it known that the different components of the enzyme system were expressed cooperatively. in order to study the essence of cellualase induction of different carbon sources, the extracellular, plasm - membrane - bound and intracellular cellulases were made to transform different soluble inducers, and the productions were analyzed by gc chromatogram. the results supported the assumption that cellobiose acted as the direct inducer or the metabolic analogue, b - gentiobiose from cellobiose acted as the true inducer through different metabolism ways in different strains

    制備細胞膜外、細胞膜、細胞內纖維素酶，用定位於這三部位的纖維素酶分別轉化底物，然後進行氣相色譜定性分析，從而探討了不同碳源之間的誘導本質，結果認為不可溶的胞外纖維素以纖維二糖為橋梁，遵循不同的代謝途徑，直接或間接地誘導了兩株不同真菌纖維素酶的合成。
  20. Furthermore, by inserting " anther box " element to the mutated area of two site - mutation promoters, another two promoters, ipmas and ipmal, were created. in order to study the chemical - inducible capacity of wild and modified pr - la promoters, a coding sequence of gus ( | 3 - glucuronidase ) gene was fused to their downstre am, and the chimeric genes were cloned into pbin ! 9 - based plant expression vector

    為了檢測得到的啟動子驅動效率及誘導活性，將所得到的啟動子、定點突變啟動子和插入花藥盒的啟動子與gus基因連接，構建了6個植物表達載體，同時分別構建包含ipl barnase 、 ipml barnase 、 ipmal barnase嵌合基因的植物表達載體。
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