接合酶 的英文怎麼說

中文拼音 [jiēgě]
接合酶 英文
joining enzyme
  • 接 : Ⅰ動詞1 （靠近;接觸） come into contact with; come close to 2 （連接; 使連接） connect; join; put ...
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 酶 : 名詞[生物化學] （生物體的細胞產生的有機膠狀物質） enzyme; ferment
  • 接合 : joint; zygosis; juncture; articulation; concrescent; nexus; coaptation; syndesis; synapsis; meet;...
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. The global regulator csra of e. coli is a specific mrna - binding protein. csra negatively regulates several metabolic pathways that are induced post - exponentially, including glycogen biosynthesis, gluconeogenesis, and glycogen catabolism ; positively controls gene expression within the glycolytic pathway ; and also csra modulates the levels of enzymes that participate directly in pep metabolism

    Csra是整體調控網路的調控基因，可負調控指數生長後期誘導的一些代謝途徑，包括糖原的生物合成、糖原的分解代謝、糖異生，而對糖酵解的一些重要基因的表達則執行正調控功能， csra也調控直接參與pep代謝的三個酶的活性水平。
  3. Among the genes, there were genes directly related to liver regeneration : fetuin, cathepsin ; close related to liver function : cytoplamic aspartate aminotranferase, gutathion sulfur transferase ; related to substance and energy metabolism : atp synthetase, ribosomal protein, and related to stress response : haptoglobin, transferrin

    這些基因中有和肝再生有直接關系的如：胎球蛋白、組織蛋白酶；和肝臟功能密切相關的如：胞質天冬氨酸轉氨酶、谷胱甘肽硫轉移酶；與物質能量代謝有關的如： atp合成酶、核糖體蛋白；以及與急相反應有關的如：觸珠蛋白、轉鐵蛋白。
  4. The results showed that the f fragment, 728bp in length, could be a new gene with a little homology to the genes coding for polyketide synthetase or fatty - acid synthetase and the b fragment, about 4kb in length, is inferred to have repeat sequences around tn5 insertion site, in which there is homology to the wa 314 right arm of the high - pathogeniciry island of yersinia enterocolitica. to reveal any pathogenicity of enterobacter cloacae b8 and its mutated strains b8b and b8f to animals, the experiment with mice was carried out

    結果顯示， f片段長度為728bp ，與現有生物數據庫的blast比較分析，發現該序列僅有局部短於1oobp的區域與polyketide合成酶基因或與脂肪酸合成酶基因有低的同源性，推測為一新基因； b片段長約4kb ，序列拼接結果推測靠近tn5插入位點部位有重復序列，對b片段tn5遠端的部分序列進行blast比較，發現它與小腸結腸炎耶爾森氏菌的強毒力島有一定的同源性。
  5. The complex formed by cnbr - activated alginate and antibody is aggregated to the surface of the paraffin - graphite - chitosan electrode by electrostatic adsorption ( coacervation ). the concentration of sjag can be detected by determining the redox current of o - aminophenol, which oxidized by h2o2 in the presence of hrp. moreover, the immunosensor shows some improved performances including high sensitivity, selectivity and less non - specific adsorption

    褐藻酸鈉?抗體復合物通過靜電吸附作用被凝集到含石墨?石蠟?殼聚糖組分的電極表面，然後與抗原和酶標抗原進行競爭反應，以鄰氨基酚為電子媒介，通過測定酶催化下雙氧水對其氧化的電流大小來間接測定抗原的濃度。
  6. Molecular docking study of the cytochrome bc1 complexed with inhibitors

    1酶復合物抑制劑的對接研究
  7. This time, using cdna of zmcdc5 as template, we amplify a sequence by means of pcr technology. and then, using restrict endoenzyme and ligase, we conjunct the 0. 8kb length dna sequence in a expression vector, pet - 30a. after induction, expression and purification, we obtained a 35. 4kda truncated fusing zmcdc5 protein which contains 267aa ( 647 to 914aa in zmcdc5 ). with the purified protein, we got its antibody and testified the antibody by means of western blotting and dot blotting

    本實驗是以zmcdc5的cdna為模板，使用pcr獲得基因片段，再通過酶切連接，將得到的0 . 8kb的基因片段構建於pet - 30a表達載體上，經過誘導表達和純化，獲得zmcdc5的融合蛋白，其中包括了zmcdc5925個氨基酸中647 914共267個氨基酸殘基
  8. The cbd tag of the fusion protein was cut by site - specific protease enterokinase at starting met of the target protein lt 27. it was released from the cbd fusion tag efficiently

    利用融合蛋白中目的蛋白lt 27與cbdtag接頭處的腸激酶特異性識別位點，用腸激酶處理粗純化的融合蛋白，可將卜
  9. As bases are added by polymerase to the starting point of a new complementary strand, known as a primer, or recognized by ligase as a match, the template ' s sequence is revealed

    當聚合酶將一個核苷酸加在新互補鏈的起始引子之後，或接合酶認定某段核苷酸鏈與原始模版配對，就可利用這些反應來得出原始模版的序列。
  10. In recent years, more and more scientists presumed that rna polymerase transcription might not occur in the nucleoplasm but in the nucleoli. nevertheless, the possibility has not been proved directly

    近幾年，有許多學者相繼提出了rna聚合酶有可能在核仁區域內發生轉錄的觀點，但是這一觀點至今還沒有得到直接的實驗證據的支持。
  11. From these results, it is inferred that the active transcription of pol iii occurs in the nucleoli and its periphery region, but not the nucleoplasm. the result will help us to further comprehend the mechanism of rna polymerase iii transcription, the way of its transcripts processing and transport, and the structural and functional relationship among the three rnapolymeraes

    本實驗為rna聚合酶在真核生物細胞核中的轉錄位點提供了較為直接的證據，這對人們進一步了解rna聚合酶的轉錄機制、加工和運輸過程及三種rna聚合酶之間的結構與功能關系具有重要的意義。
  12. Studies suggest that it probably interacts directly with the viral polymerase, and we want to confirm that by mutagenesis and by enzymatic activity assays, " said tao

    有研究表明，核蛋白可能會和病毒的聚合酶直接發生相互作用，我們將通過人工誘變技術及酶活性測定等技術來核實這一說法。 」
  13. A conservative motif, recognized by proteinases of potato virus y, was inserted between nib and ppiv, which will release functional ppiv from the fused protein after infection by potato virus y. then, plant expression vector pnpa was constructed by ligating the fusing gene and pbi121, which is knocked out gus gene

    以馬鈴薯y病毒的復制酶（ nib ）基因為模板，通過聚合酶鏈式反應獲得nib基因，並在nib基因末端保留了在病毒基因組中nib與外殼蛋白（ cp ）基因相銜接的保守序列。
  14. Nitrate is converted to ammonium by nitrate reductase and ammonium is then incorporated into glutamine and gluamate, either by the glutamine synthase - glutamate synthase ( gs - gogat ) pathway or by glutamate dehydrogenase ( gdh )

    硝酸鹽在硝酸還原酶作用下被轉化為銨，接著所產生的銨在谷氨酰胺合成酶-谷氨酸合酶（ gs - gogat ）或谷氨酸脫氫酶（ gdh ）的作用下與谷氨酰胺和谷氨酸結合。
  15. It is useful for new drug design. ( 3 ) the docking approach was applied to predicate the binding of hiv - 1 integrase and its inhibitor aurintricaboxylic

    （ 3 ）運用分子對接方法對hiv - 1整合酶與其抑制劑金精三羧酸的結合過程進行了研究和預測。
  16. Both rad6 and ubcls are ubiquitin - conjugating enzymes, and mms2 is a ubc - like protein. radls and rad5 are both single strand binding ring linger proteins. rad6 binds to radls and mms2 - ubcls binds to rad5

    在泛素系統中起主要作用的是三種酶：泛素激活酶e1 ；泛素綴合酶e2 / ubc和泛素連接酶e3 。
  17. Based on a 3. 1kb pst i fragment of genomic dna of a wild s. avermitilis, a 1. 5 kb apramycin resistance fragment was inserted into sph i site of avec gene in the 3. 1 kb fragment, then a recombinant plasmid pc05 was obtained by introducing above inactivated avec fragment into mcs region of phjl401. competent cells of et12567 were transformed by recombinant plasmid pid03 and pc05 respectively

    以含有avec基因的3 . 1kb基因組dnapsti片段為基礎，將1 . 5kb的安普黴素抗性基因片段插入到avec基因中的sphi酶切位點，再將此插入失活的avec基因片段連接到具有接合轉移功能（含有orit基因）的鏈黴菌-大腸桿菌穿梭質粒phjl401的多克隆位點區，由此得到重組質粒pc05 。
  18. Benzyl chloride were used for extracting genomic dna of aspergillus. niger 14, about 1. 5kb specific fragment was obtained from genomic dna of aspergillus. niger 14 by pcr amplification with primers ( forward primer5 " ataggcatcatgggcgtctct3 " reverse - primer5 " cagctaagcaaaacactccgc 3 designed according to the known sequences of the phytase gene in the gene bank and pyrobest ? dna polymerase, after ligated with pmd18 - tvector, transformated into e. colidh5a competent cell successfully. 3. nucleotide sequence analysis of the cloned fragment revealed the presence of the whole phya gene in pcr product

    用氯化芐法提取了aspergillus . niger14 ~ #基因組dna ，根據genebank中已知的黑麴黴植酸酶基因序列設計出一對特異性引物（上游引物： 5 ataggcatcatgggcgtctc3下游引物： 5 cagctaagcaaaacactccgc3 ） ，採用pyrobest ~ （ tm ） dnapolymerase （高保真dna聚合酶） ，通過pcr方法從aspergillus . niger14 ~ #基因組dna中擴增出了預期的1 . 5kb左右的特異性產物，將其與pmd18 - tvector連接后，轉化e . colidh5菌株的感受態細胞，經質粒抽提、酶切鑒定，確認該目的產物已得到成功克隆。
  19. A 1. 5 kb apramycin resistance fragment was inserted into nru i site of aved gene and the inactivated aved gene fragment was then introduced into mcs region of phjl401 - an e. coli / streptomyces shuttle vector with conjugation function ( containing orit gene ). as a result of above procedures, a recombinant plasmid pid03 was obtained

    將1 . 5kb的安普黴素抗性基因片段插入到aved基因中的nrui酶切位點，再將此滅活的aved基因片段插入到具有接合轉移功能（含有orit基因）的鏈黴菌?大腸桿菌穿梭質粒phjl401的多克隆位點區，由此得到重組質粒pid03 。
  20. The recombinated plasmid was named pu54. based on this material, 4 recombinated plasmids pu54 - a, pu54 - b, pu54 - c and pu54 - d were construted, which contained different cloning segments of ul54 gene. 11 m1gs ribozyme containing different gss designed to the target sequences of ul54 mrna were construted by pcr

    從hcmvdna聚合酶ul54基因序列中搜尋了11個適合gs互補的靶位，人工核酶m1gs轉錄模板dna包含有t7啟動子、 m1rna基因、連接序列以及gs序列四個組成部分。
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