接種體物 的英文怎麼說

中文拼音 [jiēzhǒngtǐwù]
接種體物 英文
inoculum
  • 接 : Ⅰ動詞1 （靠近;接觸） come into contact with; come close to 2 （連接; 使連接） connect; join; put ...
  • 種 : 種名詞（姓氏） a surname
  • 體 : 體構詞成分。
  • 物 : 名詞1 （東西） thing; matter; object 2 （指自己以外的人或與己相對的環境） other people; the outsi...
  • 接種 : [醫學] vaccinate; have an inoculation; inoculate
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ （ 95 ）株接種spf雞胚繁殖病毒，經處理后提取病毒的基因組rna ，參考國內外發表的ndv融合蛋白基因序列，設計一對特異性引物，經反轉錄聚合酶鏈式反應（ rt - pcr ）擴增出約1700bp大小的特異性片段，將此片段回收純化后，利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中，再轉化大腸桿菌jm109感受態細胞，轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss

    將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。
  3. Furthermore souls from next 11 star gates towards the boundary of the great central sun are joining each kingdom along with the master oversouls to ensoul the physical and light body body - double planes concurrently of each species

    而且，來自領向大中樞太陽界域的接下來11個星空門的靈魂，正在加入到每一個王國中，和大師級超靈一起同步賦靈于每一個物種的物質層和光體翻版體。
  4. The results of biological tests have demonstrated that allantoic fluid of the first passage virus did n ' t produce macroscopic pathogenic role to chicken embryos and after passaged for four times, gross lesions were observed in chicken embryo. the virus showed typical coronavirus under electron - microscope and it could n ' t form plaque in cef cells and could hemagglutinates chicken red blood cells after treatment with 1 % trypsin. to surprise, the virus replicated in cef cells also showed hemagglutination activity to chicken red blood cells. in addition, the spf chickens which inoculated with the virus isolated from the chicken damaged tissue showed clinical sign and grow lesion, but it ' s gross lesion did n ' t resemble to those of field outbreaks

    生物學特性：雞胚尿囊液經離心、磷鎢酸負染后，電鏡觀察該病毒為典型的冠狀病毒；該毒株的第一代尿囊液對雞胚無肉眼可見的致病作用，當繼代到第5代后，胚體嚴重病變；病毒在雞胚中隨著接種時間的延長，其效價增高， 96h可達到48h的2倍；該毒株可在cef上生長，但不能形成明顯的蝕斑；經1胰酶處理后可凝集雞紅細胞；雞胚的第四代尿囊液病毒回歸動物體，病死雞腎臟呈典型的花斑腎，腺胃則未見肉眼可見的病變。
  5. The swine manure was mixed with two kinds of anaerobic activated sludge at the mass ratio of 3 : 1 : 1 and the mixture was used as inoculum in the batch experiment

    將豬糞與兩種厭氧污泥按質量比3 : 1 : 1混合作為接種物，試驗了纖維素、葡萄糖和乙酸作為磷酸鹽還原反應電子供體的可行性。
  6. According to medical research, when vitamin b12 enters the body, it forms a compound with an intrinsic factor secreted by the parietal cells large cells of the peptic glands on the gastric mucosa mucous membrane of the stomach, before being absorbed by receptors in the ileum lower part of the small intestine in the presence of calcium ions

    在維他命b12的吸收方面，根據醫學報導，維他命b12在吃進人體后，首先會在胃部，和胃壁細胞parietal cells分泌的一種蛋白質內因子intrinsic factor結合，形成復合物后，再由小腸段中的回腸ileum吸收。在回腸之接受體receptors吸收時，需要有鈣離子之存在。
  7. Reclamation, purification and linkage of them respectively, then sequencing and analyses of according gene structure were made, results show that the complete sequence length of corresponding pcr product from brussel sprouts, kohlrabi and kale are 1665bp, 1650bp and 1650bp, containing the first two exons and introns and 22bp of the third exon

    對各pcr產物分別進行回收、純化、載體連接和序列測定及基因結構分析等，結果表明，該片段在抱子甘藍、羽衣甘藍和球莖甘藍三種作物中的全長分別為1665bp 、 1650bp和1650bp ，包括相應基因的前兩個外顯子和內含子以及第三個外顯子的22bp序列。
  8. The histologic lesions were characterized by the remarkable atrophy of intestinal villi, basal vacuolation of enterocytes, increment of the crypt and the villus - to - crypt ratio had significantly decrease. compared with that of noninoculated control chicks. besides. intestinal villi become thin and flat. or cubic, also accompanied with necrosis. many villous enterocytes were detached from the lamina propria and the lamina propria contained a diffuse infiltrate of lymphocytes, plasma cells, macrophages. the characteristic ultrastructural changes observed by electronmicroscope were swelled mitochondria and dilated endo - plasticreticulum. ribosome on the surface of endo - plasticreticulum fall off and the number of ribosome within the cell decrease

    動物回歸試驗中接種兩周齡spf雞， 24h后陸續發病，表現為持續性水樣腹瀉；剖檢可見病雞脫水、小腸內有大量的液體和氣泡、腸粘膜變薄；組織學變化為腸絨毛上皮壞死、脫落，絨毛平均長度減少而隱窩深度增加，固有層中淋巴細胞浸潤。其臨床癥狀及病理組織學變化與自然發病相同。
  9. Based on the previous studies, the research in this paper was carried out, mainly including two parts as follows : ( 1 ) anammox bacteria and aerobic ammonia oxidizers were detected in situ in 6 sediment samples taken from jiangsu province. molecular techniques, such as fish, pcr, dna cloning and sequencing etc. were used for this purpose. ( 2 ) the continuous cultivation of anammox bacteria from sediment samples were studied, which provides experimental basis for the bioaugamentation of eutrophicated sediment applying anammox process

    本論文在前人研究的基礎上，開展了以下兩個方面的工作： （ 1 ）採用分子生物學技術熒光原位雜交（ fish ） 、多聚酶鏈式反應（ pcr ） 、 dna克隆和測序等對采自江蘇省蘇州市、東太湖、新沂河等6個底質樣品進行了厭氧氨氧化菌和傳統氨氧化菌的原位檢測； （ 2 ）探討了以底質作為接種體進行厭氧氨氧化菌富集培養的可行性，為天然底質環境中厭氧氨氧化過程的強化，富營養化底質微生物修復的可行性提供一定的依據。
  10. The information generated in current study suggests that the developing influenza ecosystem in southern china region may play an important role in the process of emerging novel influenza viruses, even directly impact the genesis of pandemic influenza strains. materials and methods : fecal, cloacal and tracheal swabs from different types of poultry were collected in live - bird retail markets once a week. they were inoculated into 9 - 11 days embryonated chicken eggs and incubated in 35 for 72 hours

    本課題意在: 1 、以pa和cu為代表，探討hgnz亞型流感病毒在這些新形成的小種群中的流行情況; 2 、探討這些小種群中流感病毒的來源:是從其它動物跨種屬傳遞而來，還是本身為流感病毒的天然宿主: 3 、探討這些新形成種群中hgnz亞型流感病毒的進化情況，以及其在整個流感病毒生態體系中的作用;材料和方法:每周採集標本一次，常規處理后，接種於9一n日齡雞胚尿囊腔， 35恆溫培養72小時，收取雞胚尿囊液。
  11. This review includes the following subjects : induction frequency of haploids depend on genotypes, developmental stage of explants, temperature pretreatment of the explants, medium composition and the conditions of cultivation

    迄今己有9個科21種植物用這一方法誘導出單倍體植株，植物的基因型、外植體的發育程度、接種前的預處理、培養基和培養條件等均能影響誘導率的高低。
  12. In order to find an explanation and to determine the possible mechanism of these biological effects, many in vitro and in vivo studies of emf effects have been published. these studies were involved in many enzymes ( such as odc, succinate dehydrogenase, pkc ), pro - oncogene ( c - myc, c - fos, etc. ), gap junction and protein synthesis, and so on

    為了揭示工頻磁場生物效應的機制，尤其是與腫瘤發生發展的關系，人們開展了較為廣泛的體內及體外的實驗研究，涉及到各種生物酶類（ odc 、琥珀酸脫氫酶、 pkc等） 、原癌基因（ c - myc 、 c - fos等） 、細胞間縫隙連接及蛋白合成等方面。
  13. The animal is accompanied by a pet passport ( for animals of european origin ) or equivalent health documentation which requires endorsement from afcd ; the animal has a valid anti - rabies vaccination status ; and, the animal has been examined 24 hours by an approved veterinary surgeon before travelling and found to be in good heath and able to withstand travel to the planned destination

    這些條件包括: 1 ）寵物必須植入晶片以辨認身分； 2 ）附有寵物護照（適用於來自歐共體成員國的寵物）或經漁農自然護理署加簽具同等效力的動物健康證明文件； 3 ）寵物已接種有效的狂犬病疫苗；及4 ）寵物在出發２ ４小時前由認可獸醫檢驗，證明健康狀況良好，即使旅程舟車勞頓，亦能應付。
  14. Influences on host plant cell pathology by tumv infection tumv particles were scattered in cytoplasm area of diseased cells separately or in bundles. the pinwheels, scrolls and laminated aggregates, which were the cross sections of cylindrical inclusion bodies, were observed under transmission electron microscope. meanwhile, pathological changes of diseased chloroplasts " morphology and structure took place

    Tumv侵染寄主的細胞病理學特徵利用透射電鏡觀察接種寄主細胞的超薄切片，分離自杭州榨菜上的tumv分離物jc - 1在青菜和芥菜的細胞質中病毒粒子分散或成束分佈；細胞質中存在不同形態的柱狀內含體，分別為風輪體、捲筒體、片層聚集體；同時，葉綠體發生了形態和結構上的改變。
  15. By the compounds of submandibular gland cells and collagen sponges. we investigate the optimal cell denisity of tissue engineered compound of submandibular gland cells and collagen sponges, the cellular compatibility of tissue engineered compound of submandibular gland cells on the collagen sponges with different porosity and the influence of epidermal growth factor on the adherence of submandibular gland cell to collagen sponge. our studies can primary provide theoretical ground work to form the model in vitro of tissue engineering smg

    在本研究中，以初步探討體外頜下腺細胞與膠原海綿支架相互作用為目的，採用體外分離培養sd大鼠頜下腺細胞，然後接種于膠原海綿支架上體外復合培養的方法；從不同接種細胞濃度對細胞一支架復合物影響，同一接種細胞濃度在不同孔隙率的支架上黏附、增殖的情況及表皮生長因子（ egf ）對頜下腺細胞的促增殖作用，促細胞在支架上黏附等三方面入手，初步研究了頜下腺細胞與膠原海綿相互作用的影響因素，為進一步在體外及體內構建較為理想的組織工程化頜下腺提供理論參數和實驗依據。
  16. 1. we summarized the methods applied to measure the temperature, expounded the problems in these methods, demonstrated the significance about how to ascertain the face of high temperature radiant body ; 2

    對國內外各種接觸式和非接觸式測溫方法和技術進行了較為系統的綜述，對其存在的問題進行了分析，並指出了被測高溫輻射體物面確定的意義和方法； 2
  17. The main effect factors include : 1. microbial species, which seem to have different degradation rate under varied circumstances and n - alkanes ; 2. physical and chemical properties of crude oil itself, which have an important effect on biodegradation, such as conglutination, boiling point, refractive index, ingredient and concentration of the oil ; and 3. culture condition of microorganism, under which the degrading activity of microorganism will be affected by surfactant, light condition, sorbent, nutrition, co - metabolite, oxygen, temperature and salinity during the inoculation of selected or indigenous microorganisms

    其中主要影響因素包括：菌種的影響，菌種在不同的環境中和對不同碳鏈長度的碳氫化合物表現出不同的降解效率；石油物質本身物理化學特性的影響，如石油物質在水體或土壤中的濃度以及石油的粘度、沸點、折射率等特性；生存環境條件的影響，在接種入高效率的降解菌或利用土著微生物進行降解時，降解率受到生存環境中各種條件的影響，如表面活性劑、光照條件、吸附劑的利用、營養鹽、共代謝底物、氧氣、溫度、鹽度等。
  18. No trace of any newly expressed protein band was noticed in supernant as well as in the cells by sds - page, except the verification of the substitution of beta - galactosidase gene ( the lose of galactosidase protein band ), which is a selective marker of the wild - type virus. elisa test results suggested the expression of egf in cells, but not in culture supernant. the quantitative calculation suggested the expressed egf was about 6 - 7 u g ( as egf standard ) per flask ( 2 > < 106 cells ) in the cellular extract

    將重組病毒rbmbacph - egf以10moi感染bmn細胞， 72小時后收集培養細胞和上清；培養上清和經超聲波處理的細胞樣品elisa檢測發現胞內樣品中存在能與egf抗體免疫反應的產物，粗略估計表達量約6 7 g 2 10 ~ 6個細胞（相當于egf標準品） ；重組病毒rbmbacph - egf穿刺接種5齡家蠶幼蟲，每隔24h收集蠶血淋巴，經elisa檢測發現第4天表達量最高，根據egf標準曲線計算蠶血淋巴的表達量約32 g ml ； elisa定性實驗還發現正常蠶血也存在與egf抗體間交叉反應的物質。
  19. The disease, low quality of embryo, semen contamination, breeding out of time, low quality of recipient, not synchronous estrus between donor and recipient, antiquated preserve to embryo, hereditary unsoundness, accidental injury by medicament, vaccination, feeding and management to depart from science, immunological reaction and multiple embryo in uterus are the more important factors

    這些因子主要包括疾病、胚胎質量差、精液污染、不適時配種、受體羊質量差、供受體羊發情不同步、胚胎保存時間長、遺傳缺陷、誤用藥物、接種疫苗、飼管不科學、免疫反應、子宮內多胚等。
  20. The comparative analysis of chinese shipping enterprises as a third party logistics provider and the actual situation of international shipping line as a third party logistics provider is made in chapter 3 ; in chapter 4, chinese tpl developing present and future prospects ; in chapter 5, i make a detailed analysis on how to make the target come true, point out that that in order to meet the target, shipping enterprises should follow the five principles : first, adopt information management system. second, improve personal qualities of whole personnel. third, offer customer - oriented services

    由於第三方物流在我國還是一個年輕的行業，近年來通過與航運第一線企業大量的密切接觸和相互交流，深深地體會到了我國傳統航運企業在新經濟的武漢理工大學碩士學位論文大潮下向第三方物流服務轉型的迫切心情，同時也發現雖然各種刊物都大量地介紹現代物流，但怎樣才能成功地轉型和開拓成為一個真正的第三方物流企業，又是大家都感到比較困擾的問題。
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