提純段 的英文怎麼說

中文拼音 [chúnduàn]
提純段 英文
rectifying section
  • : 提動詞(垂手拿著) carry (in one's hand with the arm down)
  • : 形容詞1 (純凈; 不含雜質) pure; unmixed 2 (純粹; 單純) simple; pure and simple 3 (純熟) skil...
  • : Ⅰ量詞(部分) section; segment; part; paragraph; passage Ⅱ名詞(姓氏) a surname
  1. Newcastle disease virus ( ndv ) strain 695, a thermostable nature avirulent strain, were replicated in embryonated chicken eggsand its rna was extracted from allantoic fluid. referred to the reported sequence of f gene, a pair of primers were designed and synthesized. f gene of ndv b95 strain was amplified by rt - pcr, the pcr products were checked by agrose gel electrophoresis and purified by agrose gel fracion method

    利用從國外引進的新城疫熱穩定性天然弱毒b _ ( 95 )株接種spf雞胚繁殖病毒,經處理后取病毒的基因組rna ,參考國內外發表的ndv融合蛋白基因序列,設計一對特異性引物,經反轉錄聚合酶鏈式反應( rt - pcr )擴增出約1700bp大小的特異性片,將此片回收化后,利用t - a克隆技術將其克隆到pgem - t - easy克隆載體中,再轉化大腸桿菌jm109感受態細胞,轉化后經分子量比較、 pcr鑒定和酶切分析篩選陽性克隆。
  2. Gene cloning provides a means of purifying and propagating specific dna segments.

    基因克隆化供了一種化和擴增特定DNA片的方法。
  3. 6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page

    將枯草芽孢桿菌超聲波破壁后的粗取物進行分鹽析、 deae - sepharose陰離子交換柱層析, blue - sepharosecl - 6b特異結合柱層析和sephadexg - 200凝膠過濾等化步驟,得到聚丙烯酰胺凝膠電泳為單一蛋白區帶,比活為1 . 46u mg的酶制劑。
  4. The conclusions indicate that trl outperformed the control group in attitude, ability, and knowledge, these two instructional forms have prominent differentiations, the research has the conclusions that : a ) trl can nurture students " attitude towards learning, enrich students " knowledge, increase their ability and making them integrating learning strategies, b ) trl can promote students " co - operative learning, make them explore actively, and it is effective to foster mutual understanding and friendship between the han nationality and the minor nationality, c ) trl can set up an learning environment to achieve students " learning aims, such as nurturing attitude, stimulating motivation, increase students " knowledge level, d ) trl is an effective way to enhance students " learning abilities, such as information - collecting, question - probing, problem - solving, e ) trl is an important way to integrate school education with social environment, to

    因此在中等職業學校實施研究性學習,不但十分必要,而且完全可能,其目的在於改變學生以單地接受教師傳授知識為主的學習方式,為學生構建了一個開放的學習環境,供了多渠道獲取知識、並將學到的知識加以綜合應用於實踐的機會,促進他們形成積極的學習態度和良好的學習策略,培養了他們的多種社會實踐能力,以便於他們能更好地適應21世紀發展的需要。本研究以哈密地區衛生學校2001級( 1 )班社區醫士專業學生為實踐對象;以小組合作研究為組織形式;以課內與課外相結合為實踐途徑;以強調對所學知識、技能的實際運用,注重學習的過程和學生的實踐與體驗為實踐的總目標;以進入問題情境階、實踐體驗階、表達和交流階為一般程序。
  5. Dig labeled probe hybridization with solid pcr product was performed as well as electrophoresis of liquid product, this method combines rna purification, pcr amplification and nucleotide probe hybridization detection together and has many advantages including better rna purity, less time consumption, reliable positive reaction and 10 times sensitivity as rt - pcr gel running detection, reduce false positive result, unpurified nucleotide requirement, loug infected plant organism can be detected by solid hybridization

    2 )結果可靠,雜交特異性誘捕目的片,同時去除了核酸中存在的pcr抑制物質,減少了因核酸取不造成的漏檢現象,結果輸出通過雙重判定保證了結果的可靠性。 3 )靈敏度高,通過雜交進行結果判定,靈敏度比傳統的rt干cr大約高10倍。
  6. The technical measures for deep reducing coal moisture were urgent needed due to china devoting major effort to implement the new and high technologies used for coal liquefaction and preparation high purity coal. through the analyses of current situation of technical level for coal drying and dewatering, the paper suggests to take overheat steam produced from power plant as a heat source, use the disk dryer to deeply reduce coal moisture with simple working process and low production cost

    本文針對我國人力實施煤炭液化和超煤制備等高新技術,迫切需要將煤中水分深度脫除的技術手,通過對我國煤炭乾燥脫水技術現狀與水平的分析,出以電廠過熱蒸汽為熱源,利用盤式乾燥機,以簡捷的乾燥工藝、低的乾燥成本,深度脫除煤中所含水分的技術方案。
  7. In the first model, by considering the " " taste " for business moral commitment as one means by which businessman can gain more interest, it is revealed that there is a relationship between labor divisions and moral commitment consequent on purely private and individualistic activity in the absence of government

    第一個模型,在假定商人對商業道德承諾的偏好是商人為獲得更多物質利益而採取的一種手這一前下,分析了在沒有政府干預的市場經濟中,粹個體經濟活動所產生的道德承諾與勞動分工的關系。
  8. At present the education on the market basically divides nearly it is 3 kinds : it is pure educational insurance, offer the educational fare during junior high school, high school and university, if the children of chinese birthday teachs insurance ( a ), what pacific ocean is sure is little danger of happy be satisfactory to both sides, the insurance of a shot in the locker of filial education marriage of birthday can offer the country to be acquired as a child even marry the insurance gold of each phase ; 2 it is to offer an university to reach the following live safe, making not only is educational expenses, if the sunshine can of new chinese birthday is little insurance offers higher education gold ( 18 - 21 year old ), take advanced courses gold ( 22 - 24 year old ), marriage gold ( 25 years old ) development gold ( 30 years old ) with annuities ( 60 years old ), 3 it is special level insurance, be aimed at junior high school, high school technically or a certain phase in the university, basically appear with the form of additional risk, number one scholar of life of person of peace and tranquility is additional risk, restful the add education annuity of birthday danger such insurance

    目前市場上的教育險主要分為三類:一是粹的教育保險,供初中、高中和大學期間的教育費用,如中國人壽的子女教育保險( a ) 、太平洋保險的少兒樂兩全險,國壽的子女教育婚嫁備用金保險甚至能供從小學到結婚各階的保險金;二是供大學及以後的生存保險,不僅令是教育費用,如新華人壽的陽光燦少兒保險供高等教育金( 18 - 21歲) 、深造金( 22 - 24歲) 、婚嫁金( 25歲)發展金( 30歲)和養老金( 60歲) ,三是專門的階保險,專門針對初中、高中或者大學中的某個階,主要以附加險的形式出現,太平人壽狀元附加險、平安壽險的附加教育年金等都這樣的保險。
  9. Extraction of large - fragment genomic dna in order to gain dna template of pcr amplification ( long pcr amplification and salvage pcr amplification ) which was high purity and large fragment, three methods were used to extract genomic dna of bacillus subtilis, i. e. low melting - point agarose embedding method, sds - proteinase k - phenol chloroform extraction method and bacterial genomic dna extraction kit method. the genomic dna of bacillus subtilis were gained by these methods, and the operated programs of the methods were improved. the results showed that the genomic dna extracted by low melting - point agarose embedding method were obviously biggest than that of another two methods

    大片基因組dna的取為了獲得用於pcr擴增(長距離pcr擴增和分pcr擴增)的高度、大片(至少為pcr產物長度的4倍)的dna模板,應用三種方法:低熔點瓊脂糖包埋法, sds -蛋白酶k -酚氯仿抽法和細菌基因組dna取試劑盒法,分別取獲得了枯草桿菌基因組dna ,並對3種方法的操作程序進行了不同程度的改進,結果表明:低熔點瓊脂糖包埋法取的基因組dna片明顯大於后兩種方法,採用0 . 5瓊脂糖凝膠電泳3h ,仍然跑不出加樣孔。
  10. Design improvement is in three stages partial, geometrical stress concentration improvement, pure casting technology improvement and a forge - weld box body design

    設計改進分三個階,即局部幾何性應力集中改進、高鑄造質量的工藝改進和鍛焊組合結構抱軸箱體的設計。
  11. This thesis presents a new audio digital watermark algorithm based on has model. the steps : first, it classifies the signals through fft ; second, calculating the tones, noise and the overall masking threshold of different phases through has, and changing the energy value of tones which are more than that of the overall masking threshold to embed the watermark information

    出了一種基於人類聽覺系統( has )模型的音頻數字水印演算法,該演算法首先對信號進行分離散快速傅立葉變換( fft ) ,再根據人類聽覺系統模型計算出各的類音、類噪音以及各的總體掩蔽閾值,通過改變大於總體遮蔽閾值的類音的能量值來嵌入水印信息。
  12. Chapter one has mainly introduced the development of the market of chinese car, and the theory of satisfaction to customer has carried on the survey. foreign study on customer satisfaction deepen and not thorough quite already, customer satisfaction research to accompany by enterprise management idea transformation of weight and produce. the basic goal that enterprises exist is to pursue the maximization of the profit or maximization of enterprise ' s value, satisfied with the centre theory to develop from theory of centre of output value to the customer progressively, just because the customer is satisfied with the appearance of the centre theory, have produced customer ' s satisfactory research

    我國的汽車市場已經步入了高速發展的快車道,市場競爭空前激烈,產品同質化越來越高、可替代性越來越強、服務模式也日趨同一化,顧客的消費行為亦由感性階進入了理性階,在這樣一個復雜、瞬息萬變的市場激烈競爭中,單的產品策略、服務策略等營銷手無疑已經無法被顧客識別,而以「顧客為中心」 ,不斷高顧客滿意度、升顧客忠誠度、培養獨特的品牌已經成為了轎車企業突圍的唯一出路。
  13. The isolation and purification of dnaase in the earthworm the earthworm dnaase was purified from the tissue extract of earthworm by denaturing the protein with low ph buffer, high temperature, ammonium sulfate precipitation, deae - cellulose ( de52 ) chromatography and ultra - filter membrane

    雙胸蚓組織中dna酶的分離化雙胸蚓組織粗取液經過選擇性酸變性、選擇性熱變性、硫酸按分鹽析、 deae一纖維素( de52 )柱層析、超濾膜分級分離后得到一個電泳的dna酶。
  14. In the base of analysis the water conservancy project ’ s management system history and actuality of our country, this paper point out some problems in the system, such as the property is not clear 、 personal exceed 、 the outlay is not enough 、 the system of insurance is not perfect ; bring forward something that can use reference in our country through introduce the water system of america 、 japan 、 holland ; then put forward the combine of construction and operation of water conservancy project, mainly study in feasibility research stage 、 design stage and building stage ; then, analysis the framework of pure - commonweal 、 quasi - commonweal and management water conservancy and point out the management system of water conservancy projects that suit in the conditions of our country. mainly in develop mixed farming 、 deepen in the system of personal 、 make correlation policy accelerate the reform of the water conservancy project system. at last, the author give some advice

    本文在分析我國水利工程管理體制歷史和現狀的基礎上,指出我國水利工程管體制存在的諸如單位性質劃分不準、人員超編、工程管理經費不足、社保體制不健全等方面問題;通過分析日本、美國、荷蘭等國的水利工程管理體制,出可供我國水利工程管理體制模式借鑒之處;進而出新建水利工程建設管理和運行管理有機結合的方法,主要從可研階、設計階、施工階進行結合;然後按照水利可持續發展戰略思想,探討適合我國國情的三類水利工程管理體制模式,分別分析公益性、準公益性、經營性三類水利工程管理體制模式,研究出適合我國國情的水利工程管理體制模式;從開展多種經營、深化人事制度改革、制定相關政策等方面促進水管單位管理體制的改革;在文章的最後,出筆者的幾點建議和展望。
  15. Firstly, the major ampullate glands were got from araneus ventricousus. total rna and mrna of ampullate glands were isolated and purified. double strands cdnas were synthesized in the assistance of amv - rt and e. coli dna polymerase i etc. by reverse transcription and replacement synthesis

    首先剝離大腹園蛛主壺腹腺,取總rna和分離化mrna ,反轉錄合成cdna ,凝膠層析除去小於400bp片,並在雙鏈cdna兩端引入ecor的銜接頭,對其進行磷酸化處理。
  16. On the first floor there is a authentic italy - style restaurant, which can offer western buffet

    一層大堂擁有正的義大利風味餐廳,可為賓客供不同時的西式自助餐。
  17. 70kb and 130kb in size respectively. though pks gene cluster for antibiotics fr - 008 was cloned, the genes for amino - mycosamine residue remained unknown. thus, attempt was also made to clone the desired gene ( s ), streptomyces sp

    首先從大量的發酵液中取分離了鏈黴菌fr - 008產生的抗生素fr - 008 ,並通過大孔樹脂吸附、柱層析等手進行了初步
  18. After the arrangement information has been obtained, the ransac method is used to get rid of false matches in the second phase matching

    獲得了圖像序列位置關系后,自動拼接演算法進行第二個階的特徵匹配,這一個階使用了ranasac演算法粗略匹配數據。
  19. The production base of our company lies in the central area of mengzhou city. it is north to the yellow river and south to the mount taihang and has convenient transportation conditions. as the first polydextrose manufacturer, we formulated our own corporate standard according to the standards of the foodstuff chemistry code and the world foodstuff and oil organization. we have good testing equipment and fully carry out this quality standard. we are trying our best to further improve our research development, production, and aiming at providing all customers with high quality products

    聚葡萄糖可溶性膳食纖維是孟州市泰利傑有限責任公司依據世界糧油衛生組織fao who ,採用高科技手,以玉米纖維口服葡萄糖山梨醇檸檬酸等多種原料,經聚合而生成的一種天然優質可溶性膳食纖維,其膳食纖維含量高達93 %以上。
  20. Sequence and phylogenetic analysis of segement a of chinese infectious bursal disease virus ( ibdv ) hb - bp strain isolated from hebei province was studied in this thesis. the experiments as below were included : double - strand rna of viral genome was purified by licl gradient precipitation, 48 hours after bursa was harvested from chicken which had been inoculated with hb - bp strain. referred to the published sequence two primers were designed and synthesized

    給4周齡spf雛雞人工接種雞傳染性法氏囊病病毒hb - bp毒株, 48小時后採取法氏囊,用雙licl分級沉澱法全長基因組dsrna ,設計一對引物,通過rt - pcr方法進行了體外擴增,獲得hb株a節基因全長cdna 。
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