插入蛋白 的英文怎麼說

中文拼音 [chārùdànbái]
插入蛋白 英文
insertin
  • 插 : 動詞1. （把細長或薄的東西放進、擠入、刺進或穿入; 插上; 插進） stick in; insert 2. （中間加進去; 加進中間去） interpose; insert
  • 入 : Ⅰ動詞1 （進來或進去） enter 2 （參加） join; be admitted into; become a member of 3 （合乎） conf...
  • 蛋 : 名詞1. （鳥類或龜、蛇類所產的卵） egg 2. （像蛋形的東西） an egg-shaped thing 3. （辱罵之詞）
  • 白 : Ⅰ形容詞1 （似雪的顏色） white 2 （清楚; 明白; 弄明白） clear 3 （空的; 沒加他物的） pure; clear; ...
  • 插入 : insert; infix; run in; break in; patch; insertion; plug in; intercalate; intercalation; intromiss...
  • 蛋白 : 1. （卵中透明的膠狀物質） egg white; albumen; gary2. [生物化學] （蛋白質） protein
  1. In order to express alkaline protease gene ( ap gene ) in bacillus subtil is, the recombinant expression plasmid was constructed. this plasmid contains a promoter bp53, also from b. pumilus un - 31 - c - 42, ap gene and the shuttle vector psugv4. after introduced into b. subtilis wb600, the transformants displayed the hydrolyzed zone on milk plate

    將來自短小芽抱桿菌un一31一c礴2的基因啟動子（ bp53片段）和脫毛蛋白酶全基因（ ap ）進行融合，然後將重組基因（命名為bpap ）插入到大腸桿菌-枯草桿菌穿梭質粒載體psugv4中，構建成表達質粒psu一bpap 。
  2. To search proteins that associate with the mouse mint protein and regulate notch signaling in nuclei, and to study the function and mechanism of mint - mediated transcription repression, yeast two - hybrid assay was used to screen proteins that interact with a fragment of mint ( f5, amino acids 2226 - 2959 ). from 4x106 yeast clones transformed with the bait plasmid and a cdna library of 9 dpc mouse embryo, fifty - one were positive for nutritional screening and p - galactosidase assay. restriction digestion identified 10 independent positive clones and these were analyzed by dna sequencing these clones represent 3 correctly fused cdna fragments, which are mint, alpha a - crystallin - binding protein i ( alphaa - crybpl ), and nuclear receptor co - repressor 1 ( n - corl ), respectively

    鑒于mwt基因編碼區較長，共有10799個堿基，故此我們將mint分為六段，分別命名為fi一f6 ，本研究以其中的f5 （ 222e959 ）和f6 （ 296s576 ）片段為研究對象，將h者分別插入pgb盯7載體中，結果顯示： mintfs可與核受體輔助抑制因子1階cori ） ， o晶狀體蛋白結合蛋白1hlphatcrybp入及mw加互作用，而f6可與igm的重鏈恆定區、泛素結合酶2l6和一個未知功能的新的蛋白基因進行結合。
  3. Layered and pillared material are a kind of multifunctional material which were developed in recent years, much attention has been paid to this kind of material for its application in ion - exchange catalysts solid state proton conductivity, nonlinear optics and physic. a lot of literature have reported the intercalation behavior of a - zirconium phosphate ( abbreviated as a - zrp ), different guest molecules inserted into a - zrp have been studied in detail, those guest molecules include amine, alcohok amino acid protein, enzyme coornadiate compound and coronal compound. the intercalation guest is restricted by their size and basicity

    層柱材料是近年來發展起來的一類多功能材料，由於其在離子交換、催化、固態質子導體、非線性光學以及醫學等方面的廣泛應用而受到國內外研究者的重視，大量文獻報道了-磷酸氫鋯zr （ hpo _ 4 ） _ 2 ? h _ 2o （ - zirconiumphosphate ，縮寫為- zrp ）的超分子插層化合物及插層性能，其中對不同的客體分子對磷酸鋯的嵌入做了詳細的報道，客體分子的種類包括氨、醇、氨基酸、蛋白質、酶、配合物、冠狀化合物等。
  4. According to above consideration, experiments was carried out as below : first, targeted gene - human serum albumin ( hsa ) gene was obtained via pcr technology. secondly, the hsa gene was liganded with a plasmidprla22 whose two arms carry dna sequences necessary for crossing with the human a - lactalbumin yac to form an integration vector prla - hsa, then the integration vector plasmid was co - transformated into the right yac - bearing yeast with another plasmid plrh33 which carrys a selective gene

    因此，本試驗首先擴增出整合在酵母基因組里的人血清白蛋白（ hsa ）基因作為目的基因，並將人血清白蛋白基因插入到一個含有人-乳白蛋白yac同源序列的重組型質粒載體，以構建整合型載體，再與另一個帶篩選基因的質粒共轉化入含人-乳白蛋白yac的酵母細胞體內。
  5. Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing

    目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。
  6. Phenotypic observations and molecular detection indicated that both the rbsdv and 14 kd vectors were effective to some extent in suppressing gene silencing, and that the s6 protein could eliminate methylated sites from the target regions of the genome

    將rbsdvs6基因組分和bnyvvrna2的14kd蛋白基因分別插入經改造的pvx病毒載體，與正常的gfp表達載體共侵染本生煙。
  7. Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid, and induced with methanol to secrete interesting peptide. the supernate of pichia pastoris culture was analysed by sds - page and western blotting. a reactive band, which the apparent molecular weight is 36kd, can be detected with sheep anti - hcmv polyclonal antibodies

    重組質粒轉化巴氏畢赤酵母， g418篩選出多拷貝插入的單克隆，甲醇誘導多拷貝插入的單克隆酵母細胞分泌目的蛋白，培養液上清經sds - page電泳分析，在蛋白質印跡中檢測到培養液上清有一表觀分子量為36kd ，能與羊抗hcmv多克隆抗體發生發應的條帶。
  8. Genetic vaccine is quite different in structure from traditional ones. the experiments show that delivery of genetic material into an animal ' s cells can trigger some synthesiztion of the encoden proteins as well as of antibodies targeted against those proteins

    核酸疫苗是將編碼某種抗原蛋白的外源基因插入某種表達載體中並直接導入的動物體內，表達抗原蛋白，並誘導宿主產生對該抗原蛋白免疫應答的革命性的新型疫苗。
  9. The results demonstrated that the momps were protective antigens and the momp - iscoms of aeromonas hydrophila could induce the host to mount satisfied immunity. a pair of primers were designed according to the published nucleotide sequence of a putative outer membrane protein gene ( omp ) of aeromonas hydrophila. with the specific primers, a target fragment about 1. 1kb was amplified from aeromonas hydrophila l316 via pcr. the target fragment was inserted into the linearized pgem - t easy vector

    根據已發表嗜水氣單胞菌的外膜蛋白基因omp的核苷酸序列設計引物，利用pcr技術，擴增、克隆了嗜水氣單胞菌l316的主要外膜蛋白基因（ momp ） ，經t a克隆，插入到pgem - t系列載體上，測序分析結果表明momp基因最長的開放閱讀框（ orf ）為1035nt ，編碼由344個氨基酸組成，分子量為36kda的主要外膜蛋白質（ momp ） 。
  10. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  11. One of them was identified as the beth gene of halobacillus sp. d8. a hydrophobicity plot of beth revealed an alteration of hydrophobic and hydrophilic segments that was characteristic for integral membrane protein, suggesting the presence of 12 transmembrane - spanning segments and belonged to bcct family

    將重組質粒測序，插入片段大小為4kb左右，通過blast比較，結果顯示含有三個orf框，其中一個為甘氨酸甜菜堿轉運蛋白，對其氨基酸組成進行疏水性分析，推測為含有12個跨膜域的跨膜蛋白，屬于bcct家族。
  12. Thirteen putative epitopes showing characteristics of antigenic epitope were found from the analysis information. using pcr, the nucleotide acid fragments encoding these putative epitopes were amplified, then cloned into the expression vector miske. the positive recombinant phage displying the epitopes were found out by using pcr, sequencing and the determination of phage plaque titer

    運用goldenkey分子生物學軟體對prrsvbj - 4結構蛋白的抗原表位及其二級結構進行了分析和比較，從中篩選13段顯示表位特徵的氨基酸殘基序列，用pcr技術擴增相應的核苷酸片段，將其插入到噬菌體表達載體m13ke ，結果預測的13個表位可在噬菌體表面得以展示。
  13. With the aim of increasing the expression and stability of mtxl, the mtxl gene originated from b. sphaericus ssii1 was cloned to a shuttle vector pbu4. two recombinant plasmids pmt9 and pmt4 were obtained, with the inserted fragments in the opposite orientations

    為了提高mtx1殺蚊毒素蛋白的表達量和穩定性，本文將來源於球形芽孢桿菌b . sss - 1的mtx1毒素基因克隆至穿梭載體pbu4上，得到mtx1插入方向相反的重組質粒pmt9和pmt4 。
  14. In short, the entry of introns into eukaryotes may have initiated an explosive new round of molecular evolution, based on rna rather than protein

    簡單的說，插入子進入真核生物后，可能啟動了新一回合爆炸般的演化，這次的主角是rna ，而非蛋白質。
  15. In 1989 we created a library of h. pylori genes by inserting selected fragments of the bacterium ' s dna into cells of e. coli

    我們在1989年時，將選取的幽門螺旋菌基因片段插入大腸桿菌的dna中，讓大腸桿菌製造幽門螺旋菌的蛋白質，以此建立了幽門螺旋菌的基因庫。
  16. Although introns constitute 95 percent or more of the average protein - coding gene in humans, most molecular biologists have considered them to be evolutionary leftovers, or junk

    其實在人類的蛋白質基因中，平均95 %以上的序列是插入子，大多數的分子生物學家認為它們是演化的殘餘物或是垃圾。
  17. The fusing gene was transformed into leaf tissue of tobacco cultivar nc89. more than 100 plants were obtained. transgenes were confirmed to be inserted into tobacco genome of r0 generation by pcr and dot - blotting analysis

    通過kpn位點與成熟番木瓜蛋白酶的cdna連成一個融合基因，並插入到pbi121的原gus位點，構建成nib -番木瓜蛋白酶融合基因的植物表達載體pnpa 。
  18. In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection

    此外，為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況，我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中，與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒，將其感染sf9細胞制備p1種子液，同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定，確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。
  19. A 1. 7kb fragment encoding ge of prv fa strain was obtained by pcr from plasmid ppge templated using a pair of the designed primers containing ecori and bamhi ' sites. the ge gene fragment cutted with ecori and bamhi was inserted into the expression plasmid pbv220 including these two endonuclease sites for constructing the recombinant plasmid pbvge. strain dh5a of e. coli contain pbvge was induced at 42 for 4 - 6hr after incubation with vigorous shaking at 30 for 3hr or so

    以質粒ppgedna為模板， pcr擴增出1 . 7kb的ge基因完整片段，將擴增產物以ecori和bamhi雙酶切后，插入原核表達載體pbv220的p _ rp _ l啟動子下游的ecori和bamhi位點間，得到重組表達質粒pbvge ，轉化了pbvge的大腸桿菌dh5a經溫敏誘導表達后，用sds - page和western - blot ，以及瓊脂雙擴散來檢測，結果表明prvfa株ge基因在原核載體上得到高效表達，表達產物約占總蛋白的17 。
  20. Firstly, the eo genes of csfv - jl and csfv - ln9912 strains were amplified by rt - pcr and nested pcr and then sequenced after cloned into t easy vector. comparing the eo sequences with other strains eo by biosoftwares, dnastar5. 0 and bioedit, the phylogenetic analyse revealed that all of the strains we have sequenced could be divided into groupl and groupii. two amino acid streches of 15 csfv strains eo, which form the rnase active site, and histidine residues essential for rnase catalysis in both ones were highly conserved. the eo protein propertys of antigen epitope, hydrophobicity, isoelectric point were also predicted by bioinformatic method

    首先，採用rt - pcr和nest - pcr技術擴增了csfv - jl株和csfv - ln9912株eo基因，插入teasy載體后測序，應用dnastar5 . 0和bioedit軟體將其與本實驗室已測其它毒株和genebank中登錄的csfv代表毒株eo基因序列進行比較，繪制了遺傳進化樹並預測了eo蛋白的抗原表位、疏水性、等電點等特性。
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