搖瓶培養法 的英文怎麼說

中文拼音 [yáopíngpéiyǎngfǎ]
搖瓶培養法 英文
shaking culture
  • 搖 : Ⅰ動詞（搖擺; 使物體來回地動） shake; wave; rock; turn Ⅱ名詞（姓氏） a surname
  • 瓶 : 1. （瓶子） bottle; vase; jar; flask 2. （姓氏） a surname
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  • 法 : Ⅰ名詞1 （由國家制定或認可的行為規則的總稱） law 2 （方法; 方式） way; method; mode; means 3 （標...
  1. Through screening a lot of mutants with the method of transparent zones and culture filtrate, the best four were obtained with high - yield of stable phb depolymerase, named as 02, 04, 09 and 14

    以青黴（ penicillium . sp ） ds9701為出發菌株，通過紫外線誘變分生孢子，採用透明圈初篩和搖瓶培養復篩的方法，獲得4個能穩定遺傳的phb解聚酶高產菌株。
  2. Specific pichia clony pcr product showed that foreign phytase gene was integrated into the host cell. the experimental results from flask fermentation and phytase activity assay indicated that phytase gene was effectively expressed by the recombinant pichia

    挑選轉化子經過bmgy搖瓶培養、 bmmy誘導發酵后，用釩鋁酸按法測定了表達產物的酶活性，結果表明重組菌株可有效表達具有生物學活性的植酸酶。
  3. The 102 strains which can produce hydrolyzed circles were obtained using alternative medium containing phytate - calcium. after being isolated and purified, these strains were inoculated into fermented medium, shaking in 28 c at 220r / min for 5 days, then their enzymatic activities were determined by ammonium molybdate - phosphate colorimertry under the condition of 37 cand ph2. 5. the result showed there were 24 strains with higher enzymatic activities among the 102 strains, after the rescreening, 7 strains were gained with enzymatic activities beyond 15u / ml and stable ability of producing acidic phytase, of which, enzymatic activity of the strain 14 was the highest, reaching 53. 86u / ml, and it was preliminarilly identified as aspergillus. niger, then numbered as aspergillus. niger 14

    用植酸鈣選擇性平板培養基從土樣中篩選出了102株能產透明圈的菌株，經分離純化后，接入液體發酵培養基， 28 、 220r min發酵5天，在37 、 ph2 . 5條件下用釩鉬酸銨法測定其所產植酸酶的活力，結果顯示，酶活較高的有24株，經再次搖瓶復篩后，酶活大於15u ml且產酶性能穩定的共有7株，其中以14 ~ #菌株的酶活最高，可達53 . 86u ml ，經初步鑒定為黑麴黴，編號為aspergillus . niger14 ~ # 。
  4. In total 102 strains of acidic phytase producing strains were selected from soil by selective plate containing calcium phytate. among them 32 strains with relatively large clear circle were purified and re - selected by shaking - culturing. after fermented for 5days at 28 c and shaking at 220r / min, the activity of phytase was determined by nh4vo4 - ( nh4 ) 6mo7o24 method at 37 c and ph2. 5 or ph5. 5

    主要結果如下： 1植酸酶高產菌株的篩選利用植酸鈣選擇性子板培養基從土樣中篩選出102株酸性植酸酶產生菌，從中挑選出透明圈較大的菌株32株，經分離純化後分別進行搖瓶復篩， 28 、 220r / min發酵5天後，在37 、 ph2 . 5或ph5 . 5條件下用釩鉬酸銨法檢測其酶活，結果發現有3株菌產酶活性較高且產酶性能較為穩定。
  5. Western blot analysis showed that rhpk - 5 ( recombinant human plasminogen kringle 5, rhpk - 5 ) protein was recognized by mab same as native hpk - 5. the result suggested that we obtained correct gene sequence of hpk - 5 and got the purified rhpk - 5. section ii : construction of pbv220 / hpk - 5 vector for obtaining high - level hpk - 5 expression system, the hpk - 5 gene was recombined with plasmid pbv220 to construct the vector of pbv / hpk - 5

    Coli ）作為宿主，經sds - page分析，篩選表達量最高的菌株作為發酵用工程菌株；用western - blot方法鑒定hpk - 5因子的免疫學活性；用搖瓶發酵的方法，研究發酵培養基的體積（溶解氧） 、組成成份及誘導起始時間和誘導持續時間對目的蛋白表達量的影響，優化hpk - 5基因工程菌的表達條件。
  6. The yeast strain 12y - 5 was identified at the species level using two yeast taxonomy systems published by. j. w. kreger - van rij ( 1984 ) [ 24 ] and j. a. barnett ( 1983 ). [ 19 ] in addition, the ability to assimilate several compounds as main sources of carbon and nitrogen was evaluated at the 500ml shake - flask and 3. 7l kfl bioreactor scales. effects of ph, temperature, concentration of dissolved oxygen and utilizable sugar on the growth rate of cells and the rate of the conversion of substrate into biomass were studied

    進而用正交試驗法對該酵母生長的培養基配方及影響生長的重要理化因素進行了較為深入的研究，在此基礎上對影響生長的重要理化因素進行了寬范圍的測驗：並以搖瓶最佳的培養條件為基礎，在3 . 7升kfl -生物反應器上進行了放大試驗。
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