整合基因組 的英文怎麼說

中文拼音 [zhěnggějīyīnzǔ]
整合基因組 英文
integrated genome
  • 整 : Ⅰ形容詞1 （全部在內; 完整） whole; all; complete 2 （整齊） neat; tidy; orderly Ⅱ動詞1 （整理; 整...
  • 合 : 合量詞（容量單位） ge, a unit of dry measure for grain （=1 decilitre）
  • 因 : Ⅰ動詞[書面語] （沿襲） follow; carry on Ⅱ介詞1 [書面語] （憑借; 根據） on the basis of; in accord...
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 整合 : commensuration
  1. In our experiment, the specific fragment was amplified from transgenic bobwhite genome dna at annealing temperature 61 by using high - fidelity pfu dna polymerase and cloned into clone vector pgem - 7fz ( + ), then sequenced. the cloned sequence was completely identical to the sequence which was issued in genbank

    本實驗採用了高保真pfudna聚合酶，在退火溫度61條件下從轉基因bobwhite品種基因組dna中擴增出特異性片段，將此片段插入克隆載體pgem - 7fz （ + ） ，經測序和序列分析表明，所擴增得到的片段含有bar基因完整的讀碼框，並且序列與genbank中發表的序列完全一致。
  2. Transformed shoots were selected on solidified medium containing kanamycin. ten kanamycin resistant transformants were obtained by direct or induction calla. these transformants were checked by pcr, pcr - southern blot and southern blot, confirmed that two positive transformants were integrated into the genome of boechmeria nivea l guad

    對所得到的抗性轉化株進行了pcr 、 pcr - southernblot 、 southernblot檢測，其中2株呈陽性，證明vp4基因已整合到苎麻基因組dna中。
  3. Results of pcr for the resisted shoot and transgenic plants showed that bt gene had intergrated into maize genome

    對抗性芽以及再生植株的pcr檢測初步證明， bt基因已整合進玉米基因組中。
  4. We produced transgenic tomato plants by an agrobacterium tumefacien - mediated method. pcr - southern blot and rt - pcr revealed integration of afgf gene in the plant genome and successful its transcription in transgenic plant cells

    我們利用農桿菌介導的轉化方法獲得了afgf轉基因番茄植物，並且通過pcr - southern 、 rt - pcr證明了afqf在宿主基因組中的整合及表達。
  5. Molecular examination and biological observation of transformed plants by growing resistant germinations we got 30 transformed plants. pcr and spot blotting hybridization analysis indicate that hsp70 anti - sense cdna had been integrated into tobacco genome. through observation and measurement we found 12 completely sterile, 9hyper - sterile, 3 partially sterile and 6 fertile and significant differences between them in stamen, height, and fruit weight

    Pcr和點雜交分析表明， hsp70正、反義。 dna己經整合到煙草的基因組中。在進行田間生物學性狀觀察時， 30株轉基因植株中有12株表現出完全不育， 9株高度不育， 3株部分不育和6株可育；不育植株在雄蕊、株高和果實重量等方面都存在明顯的差異。
  6. Css is the one of the colonization factor antigens which is a protective antigen can cause immune reaction by the means taking orally. in this study, carrot was separately transformed with agrobacterium tumefacience strain lba4404 that contains ctb or ctb - cs3 fused gene in order to get oral diarrhea vaccine. this is a potent strategy to produce etec oral vaccine

    本研究期望通過農桿菌轉化系統將ctb （霍亂毒素b亞基，可作為佐劑和載體）基因和ctb - cs3融合基因分別轉入胡蘿卜植株，使ctb和ctb ? cs3融合基因穩定整合到胡蘿卜基因組內，希望以此獲得以胡蘿卜為受體的etec口服疫苗，使服食者在進食的同時就可獲得腹瀉免疫。
  7. Different amount of copies in different tissues attribute to the different density of positive signals. the result of the experiment suggested that the transgenic animals can be produced by spermatozoa - mediated gene transfer after the entrapment of liposome. and because the exogenous dna occurs losing the segments. partly integration, or existin g outside of genome dna, the rate of chimerism is relatively high

    結果表明： （ 1 ）脂質體包裹外源基因轉染精子的方法，可將外源基因導入受精卵中，能夠獲得轉基因動物，並得到了較高的轉基因陽性率； （ 2 ）精子攜帶的外源dna的整合過程是隨機的，在受精過程和胚胎早期分化過程中可能發生了片段丟失、不完全整合或游離于基因組存在而產生嵌合體。
  8. It is possible that exogenous dna fragment integerated into acceptor genome, changing gene base sequence or base deletion or base insertion, inducing to mutant at gene level

    這可能是外源dna片段整合進受體的基因組中，改變基因的順序或者引起基因堿基的缺失、插入，從而在基因水平上發生突變。
  9. Some microorganisms are evolutionary puzzles in that their genomes contain encrypted genes that are descrambled into gene products

    一些微生物基因組中包含的排列雜亂的基因密碼會重新整合併生成相應產物，這是一個進化之謎。
  10. Four hyg - resistant plants, which were chosen randomly from the plants which grown in the field, were detected through gus and pcr. the results confirmed that the npr1 gene have been induced into the genome of eustoma russellianum

    隨機選取4株移栽成活的具有hyg抗性的洋桔梗植株進行gus染色和pcr分子檢測，均為陽性，初步證明外源npr1基因已整合到洋桔梗基因組中。
  11. 4. after having established genetic transformation system with tomato cotyledons as explant and determined the transformable of preculture time, incubation time and co - culture time, we set up the system of high frequency transformation of tomato cotyledons. then hbmp - 3m gene was transferred into tomato via agrobacterium - mqdiated transformation, and the resistant plants to hyg were obtained. by pcr analysis on part of the putative transformants, we identified that hbmp - 3m gene had been integrated into the genome of part of tomato plants. 5. transferred hbmp - 3 gene into tobacco via agrobacterium - mediated transformation and obtained the resistant plants to hyg. trans genie tobacco plants were confirmed by instantaneous expression of gus gene in calli detection, growth and bio - morphology analysis, hyg - resistant experiment and pcr analysis

    通過pcr檢測證實部分番茄抗性植株中已導入hbmp - 3m基因；人骨形成蛋白一3成熟膚基因和全長基因分別轉化番茄和煙草的研究5 .通過農桿菌介導法將hbmp一3全長基因導入煙草，並且獲得了hyg抗性植株，通過gus基因瞬時表達檢測、轉化植株的生長情況及形態學分析、 hyg抗性鑒定及pc尺檢測，證明目的基因己經整合到煙草基因組中。
  12. According to above consideration, experiments was carried out as below : first, targeted gene - human serum albumin ( hsa ) gene was obtained via pcr technology. secondly, the hsa gene was liganded with a plasmidprla22 whose two arms carry dna sequences necessary for crossing with the human a - lactalbumin yac to form an integration vector prla - hsa, then the integration vector plasmid was co - transformated into the right yac - bearing yeast with another plasmid plrh33 which carrys a selective gene

    因此，本試驗首先擴增出整合在酵母基因組里的人血清白蛋白（ hsa ）基因作為目的基因，並將人血清白蛋白基因插入到一個含有人-乳白蛋白yac同源序列的重組型質粒載體，以構建整合型載體，再與另一個帶篩選基因的質粒共轉化入含人-乳白蛋白yac的酵母細胞體內。
  13. The development of gene targeting and animal cloning thechnology makes the site - specific integration of foreign gene become possible

    隨著基因打靶技術和動物克隆技術技術的發展，外源基因在高等哺乳動物基因組中的定點整合成為了可能。
  14. In actual engineering applications, its wear resistance reduces due to the high temperature resulted from the continuous and heavy load service, which cannot fit the demand of engineering applications. based on the recent research throughout the world, in order to improve mechanical properties and wear resistance at high temperature of za27 alloy, si and mn are added to modify its microstructure. its microstructure and mechanical properties are tested and the sliding friction at ambient temperature and sliding friction at high temperature of znalsi and znaimn are studied

    本文研究了za系合金中應用最廣泛的za27合金，針對企業在工業應用過程中發現za27在中低速重載連續工作時，因溫升造成耐磨性下降，難以滿足工況要求的實際情況，為進一步提高其力學性能和高溫耐磨性，結合國內外研究現狀，在za27合金基礎上，添加si 、 mn合金元素調整合金的組織，通過顯微組織分析和常溫機械性能測試，以探索znalsi及znalmn合金常溫及高溫滑動摩擦條件下的摩擦特性。
  15. To facilitate the detection of gene replacement, apr gene was placed in the middle of the two inserts. orit from e. coli plasmid rk2 was also incoporated into the vector, therefore, pij903 derivatives can be mobilized from e. coli into streptomyces sp. fr - 008 by either bi - parental conjugation or by conjugation between streptomyces

    為了在克隆完整基因簇時避免這兩方面的影響並能隨機地獲得dna大片段，我們將第二代yac載體pjs97 - pjs98改造成為適合於在白林泉鏈黴菌基因組中dna大片段的基因置換與克隆鏈黴菌中分析被克隆大片段功能的基因置換載體。
  16. Pcr, pcr - southern blot analysis, southern dot blot analysis of lettuce dna confirmed that adw gene had been integrated into the plant genome. the results also showed that the transformation frequency of pb - adw was higher than that of pbg - adw, which suggested that camv35s promoter would be better than pi ii promoter in the case of transgenic lettuce

    （ 3 ）細胞核載體pb - adw 、 pbg - adw均採用農桿菌介導法將adw導入萵苣，細胞核轉化獲得了生長良好的抗性萵苣植株，經pcr 、 pcr - southern 、 southern斑點雜交分析證實， adw基因已整合到萵苣基因組中。
  17. They will then integrate the data with the human genome sequence to provide a key research resource

    然後他們將用人類的基因組序列來整合資料，以提供關鍵的研究資源。
  18. It is very important to investigate and clone related genes under fe - deficiency stress in malus xiaojinensis, which not only provides the probability for exploring molecular mechanism of the absorbability and transport of fe, but also may enhance the resistance under fe - deficiency stress of plants by inserting related genes into chromosome genome

    因而研究和克隆缺鐵脅迫相關基因不僅為進一步研究蘋果吸收、轉運鐵的分子機制提供基礎。其次，在提高植物對鐵脅迫抗性的遺傳育種中，可以利用基因工程技術，將鐵脅迫相關基因整合到作物染色體基因組中，從而達到有效控制鐵營養的目的。
  19. 3. southern blot analysis of pcr positive plants indicated that 3 plants were transgenic plants and the thymosin gene has been transformed into genome of lettuce. 4

    Ctab法提取pcr陽性株的基因組dna進行southern雜交，其中3株為陽性，表明胸腺肽基因已經整合到生菜基因組中。
  20. The recombination expression vector, ppic9 - e2, was linearized by sal i and electroporated into p. pastoris gs115. recombinant p. pastoris gs115, designated gs115 - ppic9 - e2, was identified by pcr and the product of the pcr was analyzed by sequencing before its expression for csfv e2 protein

    重組表達載體ppic9 - e2經sal酶切線性化后，電轉化整合到畢赤酵母gs115基因組上，經pcr鑒定和pcr產物測序分析，陽性轉化子命名為gs115 - ppic9 - e2 。
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