斑點雜交 的英文怎麼說

中文拼音 [bāndiǎnjiāo]
斑點雜交 英文
dot blot hybridization
  • : Ⅰ名詞(斑點; 斑紋; 污點; 瑕疵) spot; speck; speckle; stripe; stain Ⅱ形容詞(有斑點或斑紋的) spo...
  • : Ⅰ名詞1 (液體的小滴) drop (of liquid) 2 (細小的痕跡) spot; dot; speck 3 (漢字的筆畫「、」)...
  • : Ⅰ形容詞(多種多樣的; 混雜的) miscellaneous; varied; sundry; mixed Ⅱ動詞(混合在一起; 攙雜) mix; blend; mingle
  • : Ⅰ動詞1 (把事物轉移給有關方面) hand over; give up; deliver 2 (到某一時辰或季節) reach (a cert...
  • 斑點 : spot; stain; speckle; freckle; fleck; dot; splodge; splotch; burl; flick; macula; measles; tache
  • 雜交 : [生物學] hybridize; cross; hybridization; cross breeding
  1. Dsmv is proved as the predominating virus - pathogen on aroid plants from zhejiang province and other regions in china. cdna of dsmv rna 3 " end partial sequence and subgenomic rna promoter region of cucumber mosaic virus ( cmv ) rna3 were used as probes for detection of dsmv and cmv respectively. total rna extracted from field samples were used for rna dot - hybridization

    用侵染馬蹄蓮的dsmv3末端序列和黃瓜花葉病毒( cmv )的亞基因組啟動子區互補dna序列為標記探針,對自然感病的天南星科植物進行rna斑點雜交,並結合雙鏈rna分析、病毒提純和形態學觀察,對杭州等地16屬天南星科植物的81個樣品進行了病毒鑒定。
  2. They make an ideal crossing breed. put to a white breed, the offspring will be white with the advantage of hybrid vigour

    格洛斯特郡豬是理想的豬種。它與白種豬,其後代將會是白色的,優勢明顯。
  3. Lastly by using the technique of dot blot hybridization, the genome dna of chlamydia was detected with the probe of momp gene labeled with dig - 11 - dutp by using the way of random primer. the results showed the degree of sensitivity of the probe was 10 pg and other pathogens could not be detected by this probe. by comparing the diagnostic ways of nucleotide probe and fc, the technique of nucleotide probe were proved to have high sensitivity and speci fi city

    最後,用地高辛隨機引物法標記成momp基因核酸探針,斑點雜交檢測衣原體基因組dna ,靈敏度可達10pg ,且不能檢出其它病原體的核酸。將核酸探針法與補體結合反應法對衣原體感染的診斷進行比較,初步證明該探針具有較高的敏感性與較強的特異性。
  4. One was cloning, sequence analysis and expression of the fragment containing the b and c antigenic sites locating at the 5 " terminus in spike gene of tgev in prokaryotic expression system ( fused with gst ), the other was preparation of non - radioactive probe labeled by digoxigenin for detecting the rna extracted from tgev by assay of dot - blot

    為了鑒別診斷tgev與prcv及對tgev進行流行病學調查,本研究採用原核表達系統( gst融合表達系統)表達tgev纖突蛋白( s蛋白)中含有b和c抗原位的多肽,並且制備了非放射性地高辛標記的核酸探針,通過斑點雜交( dot - blot )檢測tgev核酸rna 。
  5. Protocol of dot blot and in situ hybridization for white spot virus

    對蝦白病毒斑點雜交和原位檢測操作規程
  6. The pbacph - egf plasmid dna was used to co - transfect bmn cell with the modified bombyx mori baculovirus bm - bacpak dna, which was first linearized by aocl. after two rounds of plaque isolation, the recombinant virus ( named bmbacph - egf ) was further verified with pcr and dot hybridization. the recombinant bmbacph - egf virus was used to infect bmn culture cells at a moi of 10

    重組轉移載體dna與經bsu36i酶切線性化的修飾型病毒bm - bacpakdna共轉染家蠶bmn細胞,兩輪空篩選和純化,獲得重組病毒rbmbacph - egf ,經pcr擴增、 dna等方法鑒定證實重組病毒中已正確插入ph - egf融合基因。
  7. To device a primer pairs and amplify the full length pstvd by rt - pcr, the positive rna extraction from tuber sample was used as template. the product of rt - pcr was purified and connected to the plasmid pmd 18 - t vector

    Cdna核酸斑點雜交反應( nash )檢測pstvd方法準確、靈敏度高,一次檢測樣品數量多,且對于異地樣品檢測非常方便,是以往其它檢測方法的有效補充。
  8. Dot blot mccallum mccallum splaques

    分子
  9. Pcr, pcr - southern blot analysis, southern dot blot analysis of lettuce dna confirmed that adw gene had been integrated into the plant genome. the results also showed that the transformation frequency of pb - adw was higher than that of pbg - adw, which suggested that camv35s promoter would be better than pi ii promoter in the case of transgenic lettuce

    ( 3 )細胞核載體pb - adw 、 pbg - adw均採用農桿菌介導法將adw導入萵苣,細胞核轉化獲得了生長良好的抗性萵苣植株,經pcr 、 pcr - southern 、 southern斑點雜交分析證實, adw基因已整合到萵苣基因組中。
  10. Expression analysis the expression analysis of gharf in different tissues of cotton was carried out by rna dot blotting with ghakf probe labeled with dig. the result showed that the expression of arf gene was mainly in anthers of sterile and fertile, pollen of fertile, corolla rather than in leaves, radicel and ovule

    棉花ari ; 』基因的表達分析用地高辛將棉花arf基因標記為探針,與棉花洞a的不育株花藥、司有株花藥、可育花粉粒、葉片、花冠、胚根和胚珠的總rna進行斑點雜交
  11. Good dams ? the gos is a milky breed and a good dam. in many cases, sows will continue breeding at a greater age than many breeds and hybrids

    繁殖率高- - - - - - - - - -格洛斯特郡豬的繁殖性能非常好。無論在什麼情況下,母豬比其它的品種和豬種使用年限長。
  12. It indicated that 76. 5 percent of positive frequency of all the plants was achieved by using southern dot hybridization, 87. 5 percent of the plants with gus positive activity was gotten by pcr southern blotting and 42 percent of the plants with gus positive activity was gotten by southern blotting

    轉基因卡那黴素抗性植株中, gus表達陽性率為43 ,隨機抽樣的方法進行殺蟲肽和npt基因的pcr檢測陽性率均為100 。經過分子生物學檢測表明, southern斑點雜交76 . 5植株表現陽性。
  13. By northern dot blotting, it also showed that the foreign gene could express in the transgenic plants

    Northern斑點雜交表明,外源基因在轉基因植株中正常表達。
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