新型串酵母 的英文怎麼說
中文拼音 [xīnxíngchuànjiàomǔ]
新型串酵母
英文
cryptococcus neoformans- 新 : Ⅰ形容詞1 (剛出現的或剛經驗到的) new; fresh; novel; up to date 2 (沒有用過的) unused; new 3 (...
- 酵 : 動詞(發酵) ferment; leaven
- 母 : Ⅰ名詞1 (母親) mother 2 (泛指女性長輩) one s elderly female relatives 3 (配套的兩件東西里的凹...
- 新型 : late-model; new type; new pattern
- 酵母 : yeast酵母浸液[提取液] yeast extract; 酵母聚糖 zymosan; 酵母片 aluzyme; 酵母細胞[植物] yeast plant; yeast cell
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In this article, the misgurin gene and adaptor are synthesized according to the amino acid sequence reported in the genebank and the need of construction and expression. adopted a new strategy, multiple copy gene is ligated in the same direction. and then it is cloned into expression vector plasmid ppic9k
本文根據genebank登錄的氨基酸序列,同時考慮構建和表達的需要,化學合成了misgurin基因和接頭,採用一種新的策略,在體外將基因多拷貝同向串連,並將其克隆于表達質粒ppic9k中,通過鑒定並測序正確后,電轉化真核表達宿主? ?畢赤酵母菌株gs115 ,通過營養缺陷型培養基篩選重組子,再利用g418抗性篩選出整合有多拷貝外源基因的重組子。
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