明膠瓊脂 的英文怎麼說
中文拼音 [míngjiāoqióngzhī]
明膠瓊脂
英文
gelatin agar culture-medium-
For these reasons, agar rapidly replaced gelatin as the hardening agent of choice for bacteriological work.
由於這些原因，瓊脂很快代替了明膠，或為細菌學研究的較理想的固化劑。Extraction of large - fragment genomic dna in order to gain dna template of pcr amplification ( long pcr amplification and salvage pcr amplification ) which was high purity and large fragment, three methods were used to extract genomic dna of bacillus subtilis, i. e. low melting - point agarose embedding method, sds - proteinase k - phenol chloroform extraction method and bacterial genomic dna extraction kit method. the genomic dna of bacillus subtilis were gained by these methods, and the operated programs of the methods were improved. the results showed that the genomic dna extracted by low melting - point agarose embedding method were obviously biggest than that of another two methods
大片段基因組dna的提取為了獲得用於pcr擴增（長距離pcr擴增和分段pcr擴增）的高純度、大片段（至少為pcr產物長度的4倍）的dna模板，應用三種方法：低熔點瓊脂糖包埋法， sds -蛋白酶k -酚氯仿抽提法和細菌基因組dna提取試劑盒法，分別提取獲得了枯草桿菌基因組dna ，並對3種方法的操作程序進行了不同程度的改進，結果表明：低熔點瓊脂糖包埋法提取的基因組dna片段明顯大於后兩種方法，採用0 . 5瓊脂糖凝膠電泳3h ，仍然跑不出加樣孔。Recovered the agarose and identified by agarose gelelectrophoresis. the producys of pcr fragments and pcambial303 plasmid ligation were transformed into e. coli ( dh5a ). the result of pcrof positive recombinant and restriction analysis demonstrated that the plant expressing vector of tps is attained
Pcr產物經回收后，經瓊脂糖凝膠電泳鑒定后，與pcambia1303連接並轉化大腸桿菌dh5a ，陽性重組子經pcr和限制性內切酶酶切圖譜分析，表明已獲得海藻糖- 6 -磷酸合成酶基因的植物表達載體。I and hindlll respectively. after the digested products were run via agarose gel electrophoresis and transferred into nylon membrane, the southern blot was carried out using the cdna of rubber tree etrl as probe. the result of the southern blot showed that a hybridization band ( - 3. 0kb ) turned up from the ecor i digested product and another band ( - 4. 8kb ) turned up from the hindi ]
從橡膠樹pr107品系的嫩葉提取基因組dna ，用限制性內切酶ecor 、 hinofll分別酶切，瓊脂糖凝膠電泳分離並轉膜后，用克隆的橡膠樹etri基因的cdna片段作探針進行southern雜交分析，結果表明， ecor酶切在約3 okb處有一條雜交帶出現， hi 。In order to investigate the role of mannose receptor ( mr ) of human sperm, the zona free hamster eggs were pre - incubated with purified mr ( pmr ) isolated from motile human sperm by mannose - agarose gel affinity chromatography. the ultrastuctural alteration and cortical granule exocytosis of the eggs were then observed by transmissian electron microscope and tritc - lca immunofluorescence microscope, respectively. the mice were immunized with pmr and the antiserum was raised. after capacitation and induction of the acrosome reaction, the human spermatozoa and oocytes were incubated with the antiserum. then the sperm penetration assay was undertaken
為了進一步探討人精於mr在精卵融合中的作用，本文採用改良后的甘露糖-瓊脂糖凝膠親和層析法分離純化人精子mr ，並將提純的人精子甘露糖受體（ purifiedmannosereceptor ， pmr ）作用於去透明帶的金黃地鼠卵母細胞，運用透射電子顯微鏡技術和羅丹明偶聯的兵豆凝集素（ tritc - lca ）免疫熒光標記技術觀察pmr對卵子的影響。分享友人