最大耐受濃度 的英文怎麼說
中文拼音 [zuìdànàishòunóngdù]
最大耐受濃度
英文
maximal tolerable concentration- 最 : 副詞（表示某種屬性超過所有同類的人或事物） most; best; worst; first; very; least; above all; -est
- 耐 : 動詞（受得住; 禁得起） be able to bear or endure
- 濃 : 形容詞1. （液體或氣體中所含的某種成分多; 稠密） dense; thick; concentrated 2. （程度深） （of degree or extent） great; strong
- 度 : 度動詞[書面語] （推測; 估計） surmise; estimate
- 濃度 : potency; thickness; concentration; consistence; strength; consistency; density
-
Effects of diverse environmental factors on the growth rate ( od4oo ) and nitrogenase activity ( ara ) of the strain w12 hi nitrogen - free culture were investigated in our experiments. the results implied that the strain w12 could easily adapt to different cultural conditions : it could use various carbon sources ( especially glucose, sucrose, malic acid, mannitol ), propagate quickly and fix nitrogen at a temperature range of 15 ? to 40 ? and at 25 - 35 ? for optimum, at a ph range of 4 to 8. 5, at a saline concentration range of 0. 01 % to 1. 5 % ; low nlv " concentration had little effect on its nitrogenase activity. ara could also be detected when it grow in the culture media with 5mmol / l ntv "
W12菌株對環境因子的適應性研究：無氮培養條件下，測定溫度、碳源、酸堿度、滲透壓對w12生長及固氮能力的影響，結果表明，在15 - 40下均能生長並表達固氮酶活性，其最適生長及固氮的溫度為25 - 35 ；能利用葡萄糖、蔗糖、蘋果酸、甘露醇等多種碳源生長並固氮，當培養基中同時存在蔗糖和蘋果酸時，細菌生長和固氮活性最強；在偏酸和偏堿的條件下（ ph4 . 5 - 8 . 5 ）均能保持較強的生長勢和較高的固氮酶活性，並能通過調節自身代謝平衡並適應環境的酸、堿性變化，使培養液趨于中性：能耐受較高的滲透壓，培養液中卜、 5 naci濃度對其生長和固氮酶活性影響不大，當naci濃度升至2時，菌株的生長勢及固氮酶活性才有所下降：低濃度的鉸對其固氮酶活性影響不大，在0The insert dna fragments are 7kb and llkb, respectively. two subclones that were designated pgr3h1 and pgr7h1 and can increase glyphosate resistance of e. coli jm109 up to 150mm glyphosate were constructed by subcloning the 2. 4kb and 3. 2 kb hind ? / psti fragments of pgr3 and pgr7 into the corresponding sites of pgem - 3zf and pbluescript. sequence analysis of these two subclones revealed a completely identical 1323bp open reading frame that encodes an epsp synthase
以pgem - 3zf和pbluescript為載體，利用限制性內切酶hind和pst構建這兩個克隆的亞克隆，從中分別得到兩個草甘膦耐受亞克隆pgr3h1和pgr7h1 ，插入片段各為2 . 4kb和3 . 2kb ，對這兩個亞克隆進行序列分析，發現二者均含有一個核苷酸序列完全相同的完整的epsp合成酶基因? ? aroa ，其核苷酸序列長為1323bp ，推導的epsp合成酶由441個氨基酸組成，兩個亞克隆的草甘膦耐受濃度最大可達150mm 。
分享友人