最小中毒量 的英文怎麼說
中文拼音 [zuìxiǎozhōngdúliáng]
最小中毒量
英文
minimal toxic dose-
No trace of any newly expressed protein band was noticed in supernant as well as in the cells by sds - page, except the verification of the substitution of beta - galactosidase gene ( the lose of galactosidase protein band ), which is a selective marker of the wild - type virus. elisa test results suggested the expression of egf in cells, but not in culture supernant. the quantitative calculation suggested the expressed egf was about 6 - 7 u g ( as egf standard ) per flask ( 2 > < 106 cells ) in the cellular extract
將重組病毒rbmbacph - egf以10moi感染bmn細胞， 72小時后收集培養細胞和上清；培養上清和經超聲波處理的細胞樣品elisa檢測發現胞內樣品中存在能與egf抗體免疫反應的產物，粗略估計表達量約6 7 g 2 10 ~ 6個細胞（相當于egf標準品） ；重組病毒rbmbacph - egf穿刺接種5齡家蠶幼蟲，每隔24h收集蠶血淋巴，經elisa檢測發現第4天表達量最高，根據egf標準曲線計算蠶血淋巴的表達量約32 g ml ； elisa定性實驗還發現正常蠶血也存在與egf抗體間交叉反應的物質。Hybridomas secreting monoclonal antibodies ( mcabs ) specific for clostridium perfringens type a enterotoxin ( cpe ) were produced by fusion of nso myelomas cells with spleen cells from balb / c mice immunized with purified cpe. wells containing hybridomas secreting immunoglobulin g ( igg ) antibodies against cpe were specifically identified by an indirect enzyme - linked immunosorbent assay ( elisa ), and two strains of elisa - positive hybridomas were selected and cloned forth by limited dilution
該純化cpe對小白鼠的半數致死量為2 . 5ug /只；該cpe豚鼠皮膚藍斑單位為2500 4000 ；引起紐西蘭白兔小腸積液的最小毒素量為25ug 。將純化的cpe ，在含0 . 4福爾馬林的的pb液中透析制備類毒素。A group of 18 leading european pharmaceutical companies and clinical research organisations backed the scrapping of “ acute toxicity tests ”, which have long been conducted in rodents to identify the minimal lethal dose of an experimental drug while universally applied in the past to win regulatory approval, the companies concluded that the tests, first introduced in 1927, had been superseded by others that were more accurate and were therefore no longer needed as a precondition for launching drug tests in humans
18家有影響的歐洲制藥公司和臨床研究組織支持廢棄"急性毒性試驗" ，長期以來，在急性毒性試驗中，嚙齒類動物被用於確認實驗性藥物的最小致死量在過去，急性毒性試驗普遍用於申請新藥試驗許可，公司認為1927年發明的試驗方法，現在可以由其它更加準確的方法代替，因此不再作為新藥測試的必要前提。Minimal toxic dose
最小中毒量分享友人