有效菌株 的英文怎麼說
中文拼音 [yǒuxiàojūnzhū]
有效菌株
英文
effective strain- 有 : 有副詞[書面語] (表示整數之外再加零數): 30 有 5 thirty-five; 10 有 5年 fifteen years
- 效 : Ⅰ名詞(效果; 功用) effect; efficiency; result Ⅱ動詞1 (仿效) imitate; follow the example of 2 ...
- 菌 : 菌名詞1. (蕈) mushroom2. (姓氏) a surname
- 株 : Ⅰ名詞1. (露在地面上樹木的根和莖) root and stem of a tree above the ground 2. (植株) individual plant; plant Ⅱ量詞(棵)
- 有效 : effective; valid; efficacious
- 菌株 : bacterial strain
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It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity
本研究中,木耳屬2個種的2個菌株在its區域表現出較高的保守性, 4種限制型內切酶的酶切圖譜沒有顯示出多態性;增加內切酶種類及供試菌株數量,有可能獲得具有多態性的限制性內切酶酶切圖譜; 9本實驗中, its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異,擴增片段長度均為650bp左右; 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析,紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法,兩者的關系是相輔相成,互為驗證The results are as following : 1 eric - pcr was for the first time applied in differentiating strains from edible fungi and proved to be more rapid and reliable than rapd in auricularia identification study. taken the similarity coefficient as 75 %, 29 strains of three auricularia species were grouped into 6 and 9 clusters by rapd and eric, respectively. eric - pcr clearly distinguished a. auricula from a. polytricha while rapd failed
在75的分類水平上, eric - pcr把29個菌株分為9組,黑木耳種和毛木耳種可以明顯區分開;而rapd只能分為6組,不能將黑木耳和毛木耳分開,說明eric - pcr是比rapd更快捷可靠的分子標記,可以有效用於木耳屬的種質資源及遺傳分類的研究,也適合於其它食藥用菌種質資源的研究; 2In order to study the mechanism of the effect of low concentration tfp on the proliferation of s. pombe, we watch yeast cells loaded with fluo - 3 under laser scanning confocal microscope ( lscm ). the fluorescence intensity reflected the cytosolic free calcium concentration. the result showed that, the cytosolic free ca2 + concentration in s. pombe cultured in ca2 + - free medium was 2 ~ 3 times lower than that in s. pombe cultured in medium containing 10umol / l ca24, while ca2 + concentration in s. pombe treated with 50umol / l tfp was 4 - 5 times higher
本文發現增加胞外鈣濃度以及低濃度( 20 100 mol l )三氟拉嗪( tfp )不但能促進野生型s . pombe細胞的增殖,而且對mfp7菌株也有同樣的效應,這說明胞外ca ~ ( 2 + )和低濃度tfp對不同遺傳型裂殖酵母細胞的增殖均有促進作用。It is the optimal time for subjecting creatine to the medium when cultured to 12h and the concentration of creatine was 0. 75 %. creatine, sarcosine and choline chloride could induce the creatinase production and creatine was the optimal inducer, but creatinine and urea could not induce the creatinase production. 3 purification of creatinase the process of creatinase purification was performed as follows : first the enzyme was completely precipitated in the range of 40 - 80 % of saturation with ammonia sulfate fraction precipitation
最佳氮源為玉米漿和蛋白腖,最佳比例為2 : 3 ,最佳濃度為1 . 6 ;加入其它碳源時有助於菌株穩定產酶; 100ml搖瓶的最佳裝液量為15ml ;肌酸、肌氨酸和氯化膽堿都能誘導菌株產酶,其中肌酸誘導產酶的效果最好,而肌酐和尿素不能誘導菌株產酶;誘導物肌酸的最適加入時間為接種培養12小時后,最適加入量為0 . 75 。Results of host - plant range test shown that all slow - growing isolates could effectively form nodules on arachis hypogaea and phaseolns vulgaris. they could not nodulate on visia sativa, and part of them could nodulate glycin soja and glycin max
宿主范圍研究表明供試花根瘤菌均能在花生上進行有效的結瘤並固氮;部分菌株能夠在野大豆和栽培大豆矮腳早上結瘤;所有供試菌株均不能在野豌豆上結瘤,但均能能在菜豆上結瘤且單株瘤數較多。L. acidophilus strains pb1, a878 mixed with enterococcus strains m1, pb2, a30, a31. the amounts of cholesterol removed from growth medium were tesed after anaerobical incubation at 37 cthe amount of cholesterol assimilated higher 40 % by pb1 + m1, pb1 + pb2 a878 + a30, a878 + a31. the result indicated that pblmixed with ml, pb2 and a878 mixed with a30, a31 can assimilate cholesterol effectively
將pb1 、 a878分別和各球菌m1 、 pb2 、 a30 、 a31接種于高膽固醇培養基中,在有膽汁和厭氧條件下, 37混合培養16 - 18h ,測定復合菌株和單菌株降低膽固醇的效果,以未接菌培養基為對照,結果表明, pb1與m1及pb2 ; a878與a30及a31的協同作用較好,降培養基中膽固醇的量都高於40以上。In this study a microbial system for biphenyl biodegradation is set up in order to investigate the effects of surfactants on biodegradation of hydrophobic organic compounds. four strains which can grow on biphenyl as the sole carbon and energy sources are selected out, in which alcaligenes eutrophus dj1 and pseudomonas sms02 are chosen as degradation strains. after studing the bioavailability of three nonionic surfactants ( op - 10, tween - 80, and triton x - 100 ), they are added into the biodegradation system of biphenyl
為了考察表面活性劑對疏水性有機污染物生物降解的影響,本論文選擇聯苯作為模擬研究體系,篩選、馴化得到四株可利用聯苯作為碳源生長的菌株;考察了聯苯降解菌株對三種非離子表面活性劑op - 10 、 tween - 80 、 tritonx - 100的生物可利用性;用高效液相色譜法測定了非離子表面活性劑對聯苯的增溶曲線;較系統地研究了加入非離子表面活性劑對聯苯生物降解速率的影響。The total amount of avermectins b were only about 0. 5 ~ 2 % of those produced by the parental strain olm73 - 12. this study suggests that t
在281個突變株中,還篩選到了2個突變株,其發酵產物幾乎全部為有效組分阿維菌素b1 , b2組分僅佔5 。In the present study, a compartment cultivation system and histochemical staining were used to investigate the influence of soil available p level, plant p status and soil organic p on the growth and metabolic activity of am fungi. differences in metabolic activity among am fungal isolates and the relationship between metabolic activity and mycorrhizal effectiveness were al so investigated. in addition, am fungi from a wide range of environmental conditions ( originally isolated from north, central and south china ) were used to study the ecological adaptability of am fungi and the influence of edaphic conditions on am fungal growth and metabolic activity
本研究採用分室根箱、組織化學等手段研究了土壤施磷水平、植物磷營養狀況、土壤有機磷對am真菌生長和代謝活性的影響;不同am真菌的代謝活性及其與菌根效應之間的關系,並對我國華北、華中和華南地區篩選出的高效菌株進行了生態適應性的比較,以期在理論上闡述宿主植物生長狀況及土壤條件對菌根真菌生長和代謝活性的調控機制,篩選出具有廣泛生態適應性的am菌株。Many studies have shown that lignin was the limited factor of straw degradation. in this study we screened a strain named b3 from the stem ' s inner bark of bischofia polycarpam airy shaw which yielded high level of ligninase. the strain b3 ' s ability in straw degradation and its effect on the next season crop ' s growth were then studied by making it into microbial agent in the potted experiment
鑒於此,本論文即從篩選高產木質素酶的菌株入手,結果從重陽木莖內皮中篩選到一株有較高漆酶(木質素酶之一)活性的菌株b3 ,並將其製成秸稈降解劑,模擬大田試驗研究了它對秸稈的降解效果及對后季作物生長的影響。Specific pichia clony pcr product showed that foreign phytase gene was integrated into the host cell. the experimental results from flask fermentation and phytase activity assay indicated that phytase gene was effectively expressed by the recombinant pichia
挑選轉化子經過bmgy搖瓶培養、 bmmy誘導發酵后,用釩鋁酸按法測定了表達產物的酶活性,結果表明重組菌株可有效表達具有生物學活性的植酸酶。The degradation rate of map was 79. 95 % in 5 days when the water content in soils was 150 % ( paddy soil ) in laboratory test. the test also showed that degradation rate in paddy soil was higher than that in upland soil
在模擬水田和旱田試驗中,加菌5天後水田土壤中甲胺磷的降解率為79 . 95 ,同時發現菌株在水田中的降解效果要好於旱田中的降解效果,含水量高有利於土壤中農藥的微生物降解。The transformed hairy roots were detected for agropine to make sure the transformation was successful. a conclusion was reached as follows : agrobacterium rhizogenes r1601 was an ideal strain for hairy roots transformation from trichosanthes bracteata voigt which exhibited extraordinary transformation rate ( 60 % ). od600 = 0. 7 was the optimum infection concentration for the tested transforming agent, with temperature at 25, ph at 6. 0, 20min of infection and 48h of co - culture and phytohormone at 1. 0mg / l being the most favourable hairy root - inducting pattern
通過實驗和對結果的數據分析,得出下面的結論:發根農桿菌r1601是較好的大苞栝樓發根的誘導菌株,它對預培養24h的大苞栝樓子葉外植體具有最好的轉化效率和發根誘導率( 60 ) ,最適的菌株感染濃度od _ ( 600 )為0 . 7 ,溫度27 、 ph值為6 . 0 、 20min的感染時間和48h的共培養,以及黑暗條件和1 . 0mg / l的激素最有利於發根誘導的環境條件。Summarizing the effects of pbmblc on the three strains, it is known that genetically engineered strain construction using this method can not get sati sfactory results, though the potency of ybt - 1535 ' s transformants was higher than ybt - 1535. because of the low toxicity level of ybt - 1535, the transformants with higher potency cannot be utilized commercially
綜合這三個菌株的實驗結果,雖然菌株ybt - 1535轉化子的綜合毒力有所提高,但那是建立在ybt - 1535較低毒力水平之上的,轉化子的毒效離實際應用相差甚遠,工程菌構建的基本思路可能需作大的改變。The results of this study are summarized as follows : immune injecting man - made salmonella propolis inactivated vaccine and avian typhoid 9r live vaccine can protect flocks from infecting salmonella and immuning commercial chichling with man - made salmonella inactivated vaccine with medication can decrease death rate of chichling
研究表明:用禽傷寒9r株活疫苗配合自製沙門氏菌蜂膠滅活苗免疫注射父母代種雞,可有效防治種雞群再次感染沙門氏菌病;發病後的商品代雛雞用藥物治療的同時用自製沙門氏菌滅活苗免疫,可有效降低死亡率。When both genes were co - expressed in e. coli, the activity of ppsa varied from 2. 1 - 9. 1 fold comparing to control, but the activity of tkta was relatively stable ( 3. 9 - 4. 5 fold ). whatever the two genes were expressed respectively or cooperatively, both could promote the production of dahp, the first intermediate of the common aromatic pathway, but co - expression was more effective on forming dahp and screened ppt - and ptp - as more effective. the results demonstrate that co - expression of ppsa and tkta can improve the production of dahp, and what ' s more, when multigenes co - expressed, the recombinant which has coordinated enzymes activity is optimum
莽草酸途徑的最優化和整體調控基因csra的敲除正是上述改變的分子基礎,同時也為三種芳香族氨基酸的基因工程菌的構建打下了基礎; 7 .在國內外首次實現了共同途徑限制性底物關鍵酶ppsa刁無『及arog與分支途徑關鍵酶基因phea的串聯高效表達,所構建的重組質粒ptga ,其ppsa 、 tkta 、 arog 、 cm和pd的酶活分別比對照提高了3 、 2 、 2 , 5 、 4 、 2 . 3倍,且其酶活比較協調一致; 8 .將ptga導入到篩選的基因敲除和基因替換菌株大腸桿菌31884 c甲b中,搖瓶發酵證實比以往所構建的基因工程菌株具有較高的phe產量和糖轉化率率,分別為0 . 448 %和22 . 4 % 。However, both the ctab and sds - ctab methods were only adequate to be used to extract dna of some strains of penicillium, and the dna bands of some strains were clear, but others were not obvious
另外一些菌株則提取不出譜帶清晰的dna 。而氯化芐法適合於供試的所有青黴菌株的dna提取,所有菌株的dna凝膠電泳圖譜帶清晰,效果很好。So it ' s necessary to select excellent amf species in the application of amf in revegetation
因此,篩選耐重金屬的高效amf菌株對重金屬污染地區的植被重建具有重要意義。Coli have been performed in this study. engineering bacteria, screened for efficient expression of pheromone 3, express the proteins in type of insoluble ( inclusion ) and soluble forms, respectively
本實驗對游仆蟲信息素在大腸桿菌中的表達進行了系統的比較與分析,篩選獲得了有效表達信息素的工程菌株,表達產物分別為包涵體與可溶性蛋白。In this experiment, to screen the epitope of e2 protein of classical swine fever virus ( csfv ) defined by the monoclonal antibody ( mcab ) all, which had been prepared in previous experiment, the mcab all was raised in mice and followed by purification, the concentration of protein was assayed by using the bca protein assay kit
本室利用e2基因疫苗制備了多株單抗,為e2的抗原表位研究提供了條件,我們以噬菌體隨機12肽庫分析鑒定了豬瘟病毒e2蛋白的抗原表位,為深入研究豬瘟病毒的抗原結構、制備更有效的診斷試劑和疫苗提供更多理論依據。分享友人