有絲分裂中期 的英文怎麼說
中文拼音 [yǒusīfēnlièzhōngqī]
有絲分裂中期
英文
mitosis metaphase-
Anaphase the stage in mitosis or meiosis when chromatids are pulled toward opposite poles of the nuclear spindle
後期:在有絲分裂或者減數分裂過程中染色單體分別朝相反的兩極運動。( 3 ) the chromosomes obtained by air - drying and chromosomes from the silkworm genital gland are similar in shape. but the numbers and the length of the chromosomes are obviously different. the chromosomes obtained from the genital gland have little difference in length, while the chromosomes of bmn cell are different in length very much
但是,在染色體的數目上有顯著的差異。另外,染色體間的長短差別也非常明顯。由家蠶生殖腺得到的減數分裂中期染色體和胚胎得到有絲分裂的中期染色體,其染色體長短差異不明顯,而binn細胞的中期染色體長短非常不一致,差異非常顯著。Sds - page and western blotting of plasmodia of physarum polycephalum showed that cyclin b was present in the plasmodia of s phase, g2 phase, prophase, and metaphase. the molecular weight of cyclin b in physarum polycephalum was around 62kda. cyclin b was gradually accumulated from s phase to metaphase, and reached the maximal level at metaphase, however disappeared in the plasmodia of anaphase and telophase
Westernblotting分析結果表明, cyclinb存在於s期、 g _ 2期、前期和中期的原質團中,分子量約為62kda , cyclinb的含量從s期到中期逐漸增加,特別是有絲分裂前期增加明顯,中期原質團cyclinb含量最高,后末期原質團中沒有cyclinb 。The reasults are summed up as following : ( 1 ) the more metaphase chromosomes of bmn cells and moderately contraction can be obtained by air - drying after cultured at the colchicine concentration 1. 0 u g / ml, 28 " c, for 6 - 9h
用空氣乾燥法和培養細胞貼片法對bmn細胞的染色體和有絲分裂進行研究發現: ( 1 )採用空氣乾燥法制備bmn細胞的染色體,在終濃度0 . 1ug / ml的秋水仙素28培養6 9h條件下可得到較多中期分裂相,染色體的凝集也比較適中。Cytoskeleton of a cultured epithelial cell. microtubules are shown in green and dna is shown in blue
處于有絲分裂間期的動物上皮細胞。綠色為微絲,紅色為肌動蛋白,藍色為染色質,中心點復製成兩個。W7 was added when the cells entered anaphase, we found that although some cells had elongated, the distribution patternof cam at central spindle was normal, and it seemed that a cleavage furrow would possibly form soon, however, these cells stopped cytokinesis at this stage, no furrow ingression followed
在細胞進入有絲分裂後期時,加入w7 ,我們發現有的細胞雖已拉伸變長,中區紡錘體處也有gfp cam的分佈,存在形成分裂溝的跡象,但分裂溝卻不繼續縊裂加深。On the other hand, the activity and function of p27 kip1 was affected by many physiological agents such as camp, il - 2, rampamycin. the expression of p27kip1 increased after - irradiationin in the fibroblastoma cells, leading to arrest in gl phase, but its expression markedly decreased after y - irradiation in the nbcc cells, and leaded to arrest in g2 / m phase. several studies have reported that p27 kip1 involved in a much wider range of cellar processes, such as differentiation, apoptosis and migration, the expression level of p27kip1 is key for its function in dna damage responses
另一方面, p27kip 』的活性水平及功能作用,因細胞類型不同而各異,又受多種因素的影響,如一些有絲分裂原camp 、雷帕黴素可以上調p27kip ,的表達,而il一2等則與p27ki 」 『的表達下降有關,受電離輻射( ir )照射的成纖維細胞瘤及用1 , 25一二經基d3處理的hela細胞系在gl期均有游離的p27腳『的積累,細胞周期阻滯在gl期;而在痣癌伽bccs )細胞中, y一射線能誘導p27吻『表達水平的顯著下調,細胞周期阻滯在g2艦期。Their flowers and fruits were removed in this experiment to enhance them to take branch roots on the stems, and then the branch roots were used as materials. five mitosis metaphase cells, with their chromosomes well scattered, were counted. chromosome number of hup is 2x = 24, and its karyotype formula is 2n = 2x = 24 = 8m + 12sm + 4st, being to " 3b " of stebbins
本實驗採取去其花、果實,促其莖節上長側根的辦法,以其側根為實驗材料,統計了5個分散較好的有絲分裂中期細胞,其染色體數目為2x = 24 ,核型公式為2n = 2x = 24 = 8m + 12sm + 4st ,核型類型為3b型。At that time, cytosolic fluorescence intensity decreased to normal level, which shows that most of cells get through the gl / s point and enter the log phase. when cultured in medium that neucl was omitted, most of the cells were synchronized at gl stage of cell cycle. with flow cytometry, we found that cytosolic cam content of gl cells was higher than that of normal cells at log stage
在激光掃描共聚焦顯微鏡下觀察不同周期時相裂殖酵母細胞中cam的濃度及分佈變化,結果表明,分裂期細胞總體熒光強度強于間期細胞;而對同一細胞內熒光強度的分析說明,間期細胞的熒光主要分佈於胞質中,細胞核內則分佈較少;而正在進行有絲分裂的細胞內熒光主要集中於赤道板處;剛完成有絲分裂的細胞內熒光則相對集中於兩端或其中的一端。Under the condition of the enhanced ultraviolet - b radiation, the phenomenon of abnormal mitosis was first discovered, such as the three - bundle > four - bundle and six - bundle division of the chromosome when the cell is in anaphase
並在uv - b誘導的小麥根尖細胞中,首次發現染色體在有絲分裂的後期到末期,分成3束、 4束和6束等異常分裂新類型。Cultured epithelial cell undergoing division. this cell is in metaphase of mitosis. microtubules are shown in green, actin is in red and mitotic chromosomes are colored blue
正處于有絲分裂中期的動物上皮細胞。綠色的是微絲,紅色為肌動蛋白,藍色的染色體正處于赤道板。Now and then, there exists the phenomenon that a few sperms penetrate into egg, while polyspermy is few
70min ,合子核進入第一次有絲分裂中期。 80min ,染色體處于有絲分裂後期。Mitotic extracts were prepared from the synchronous metaphase plasmodia of physarum polycephalum. the results of western blotting indicated that cyclin b was a component of the mitotic extracts of physarum polycephalum
我們自同步化生長的多頭絨泡菌中獲得了有絲分裂中期提取物, westernblotting分析表明提取物中含有cyclinb 。The nuclei of the late g2 phase treated by cytochalasinb were incubated in the mitotic extracts harvested from the metaphase plasmodia. western blotting analysis showed that the content of cyclin b in the nuclei changed little. in contrast, in the control group, the content of cyclin b gradually increased with the prolongation of incubating time
將經細胞鬆弛素b處理后的g _ 2晚期細胞核在有絲分裂中期提取物中溫育,經洗滌后對細胞核中cycli b的含量進行分析,發現隨著溫育時間的延長,細胞核中cyclinb的含量沒有明顯的變化。Mpf ( m phase promoting factor ) which is made up of cdc2 as the active subunit and cyclin b as the regular subunit is a key factor in cell division. cdc2 is the active part of the mpf, which can active multiple ser / thr
Mpf ( mphasepromotingfactor )是細胞分裂的中心調控因子,由催化亞基cdc2和調節亞基周期素b ( cyclinb )組成,其中cdc2是mpf的活性單位,具有絲蘇氨酸蛋白激酶活性。After the nuclei of late g2 phase were incubated in the mitotic extracts harvested from metaphase plasmodia, the cyclin b in the nuclei gradually accumulated with the prolongation of incubating time. while, in the control group, the content of cyclin b changed little. this indicated that the mitotic extracts of metaphase plasmodia had the activity of accelerating the process of prophase, and the cell free system with mpf activity was established
將g _ 2晚期細胞核溫育于中期提取物中,免疫印跡結果表明隨著溫育時間的延長,細胞核中cyclinb的含量逐漸增加,而在沒有中期提取物的對照組細胞核中cyclinb含量並不隨著溫育時間的延長而增加,說明多頭絨泡菌中期提取物具有促進有絲分裂前期進程的作用,同時也說明我們構建了具有mpf活性的非細胞體系。This is a very ambitious but potentially far more comprehensive and long - term approach to combating cancer than anything currently available or in development : total elimination of the genes for telomerase and alt ( alternative lengthening of telomeres ) from all of our mitotic cells
這是一次很有雄心的、但比起目前已有的或正在發展中的與癌癥戰斗任何方法,都潛在地遠為廣泛和長期的方法:從所有我們有絲分裂細胞中全部剔除端粒酶基因和消除alt (端粒變長的另一種途徑) 。Polyclonal anti - cdc2 antibodies, anti - cdc25c monoclonal antibodies and peroxidase - conjugated horse anti goat igg were purchased from sigma chemical co. ( stlouis, mo ) reagents [ a - 32p ] dctp and [ r - 32p ] atp were purchased from yahui biotech - nology co. ltd, china
在真核細胞中,進入分裂其是依賴于mpf ( mphasepromotingfaetor ) , mpf由edez激酶和eyelinb組成,通過磷酸化在有絲分裂期起作用的底物而發揮作用。As cyclin b is the mark protein representing the process of prophase, we infered that cytochalasin b destroyed the polymerization of the nuclear actin and directly affected the process of prophase. nuclear actin played an important role in the process of the chromosome construction
因為cyclinb是有絲分裂前期進程的標志性蛋白,所以我們認為細胞鬆弛素b破壞細胞核內肌動蛋白聚合后,直接影響了有絲分裂前期的進程,進而悅明核內肌動蛋白在有絲分裂前期染色體構建過程中起著重要作用。Cyclin b can be a kind of mark protein which represents the process of prophase
細胞核中cyclinb的含量變化可以作為有絲分裂前期進程的標志。分享友人