未抽提完的 的英文怎麼說
中文拼音 [wèichōudīwánde]
未抽提完的
英文
unspent-
Constructing cdna expressing library of erythrocytic plasmodium falciparum from hainan : the total rna was obtained by using. triplix kit. a modified oligo ( dt ) primer ( cds ffi pcr primer ) was used in the single - stranded ( ss ) dna synthesis reaction. the ss - dna was reversely transcripted from total rna. double - stranded ( ds ) cdna was amplified by long - distance ( ld ) pcrafter the digestion with proteinase k and sfi i, the cdna with no less than 200bp was collected and purified by glass - milk kitthe library was constructed after the ligation of cdna to tiplex2 phage particle packaged with the packaging extract system in vitro. a high titer and high recombinant ratio of cdna library was constructed
構建惡性瘧原蟲海南株紅內期cdna表達文庫提取紅內期惡性瘧原蟲海南株總rna ，直接以總rna為模板使用cdna文庫構建試劑盒，首先反轉錄合成ss一dna ，再擴增合成ds一dna （ cdna ） ，對擴增產物用蛋白酶k消化及左z丁i酶切，抽提蛋白、去除rna后，用玻璃奶試劑盒純化、回收20obp以上的片斷，經與載體連接再用蛋白包裝物包裝后形成未擴增文庫，最後擴增完成惡性瘧原蟲海南株紅內期cdna表達文庫的構建。Mtrcl has advised us that such retrofitting works cannot be advanced as they involve major alterations to the stations, and the tunnel ventilation, air - conditioning and smoke extraction systems. added to the complexity of the works is the need to carry out the works during the non - operating hours between 2 am and 5 am
地鐵公司表示由於工程涉及車站及隧道通風系統、冷氣系統及抽風系統的重大修改，因此未能提早完成。此外，所有工程只能在非行車時間內即凌晨二時至五時期間進行。
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