染色質核蛋白 的英文怎麼說
中文拼音 [rǎnshǎizhíhédànbái]
染色質核蛋白
英文
chromonucleoprotein- 染 : Ⅰ動詞1 (用染料著色)dye 2 (感染) catch [contract] (a disease) 3 (沾染) acquire (a bad hab...
- 色 : 色名詞[口語] (顏色) colour
- 質 : Ⅰ名詞1 (性質; 本質) nature; character; essence 2 (質量) quality 3 (物質) matter; substance;...
- 核 : 核構詞成分。
- 蛋 : 名詞1. (鳥類或龜、蛇類所產的卵) egg 2. (像蛋形的東西) an egg-shaped thing 3. (辱罵之詞)
- 白 : Ⅰ形容詞1 (似雪的顏色) white 2 (清楚; 明白; 弄明白) clear 3 (空的; 沒加他物的) pure; clear; ...
- 染色 : dye; dyeing; colouration; tintage; tinging; dyschroia; colouring; colour; [半] decoration染色不足...
- 蛋白 : 1. (卵中透明的膠狀物質) egg white; albumen; gary2. [生物化學] (蛋白質) protein
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In eukaryotic cells, dna is located in nucleolus in the form of chromatin by combining with histone proteins
在真核生物中, dna分子纏繞在組蛋白上形成染色質保存在細胞的細胞核中。Methods : hyperosmotic pressure animal model was established by administering 3 % sodium chloride as drinking water to rats or increasing osmotic pressure of the culture medium. osmoregulation positions in the brain, reciprocal projection pathways between the medullary visceral zone ( mvz ) and supraoptic nucleus ( son ) or hypothalamic paraventricular nucleus ( pvn ), oscillation of intracellular calcium in cultured neurons and astrocytes were studied by means of anti - fos, glial fibrillary acidic protein ( gfap ), tyrosine hydroxylase ( th ) or vasopressin ( vp ) multiple imrnunohistochemical staining, immuno - electronic microscope, wga - hrp retrogradely tracing and cell culture methods. results : ( 1 ) fos positive neurons within the mvz, parabrachial nuclei, locus ceruleus, pvn, son, subfomical organ increased markedly
方法:通過給予大鼠飲用3氯化鈉或提高培養基滲透壓濃度的方法復制高滲刺激模型,主要採用抗fos 、膠質原纖維酸性蛋白( gfap )和酪氨酸羥化酶( th ) (或加壓素? vp )免疫組織化學多重染色、免疫電鏡、 wga - hrp束路追蹤結合免疫組織化學多重染色、細胞培養等實驗方法,系統觀察了中樞參與滲透壓反射的調控部位、下丘腦視上核( son )神經元? ast超微結構的變化、延髓內臟帶( mvz )和son及下丘腦室旁核( pvn )之間往返投射通路和神經元的性質及其與ast的關系、培養神經元和ast內鈣波的變化。After the recombinant plasmid pcdna3. 1 / ts87 was identified by digestion of hindlll and bamh i, it transformed into cos7 by lipofectamine. expression product was identified by immunohistochemical method, sds - page and western - blot. the immunocytochemistry result has shown that specific brown - staining grains were found in the cytoplasm of cells transformed by recombinant plasmid versus not seen in cells transformed by pcdna3. 1 or normal cells ; the sds - page result has revealed that a band about 3 8kb was found in cell lysis transformed by recombinant plasmid versus not in cells transformed by pcdnas. l or normal cells ; the western - blot result has showed that only the band about 38kd was recognized by sera from rabbit infected by t. s artificially and sera from rabbit immunized with soluble antigen of t. s and with protein expressed by ts87 gene and by a monoclonal antibody of t. s
通過細胞的免疫組化,細胞裂解物的sds - page電泳, westem - blot分析檢測目的基因的表達情況。免疫組化結果顯示:重組質粒轉染的細胞質中有棕褐色顆粒,而空載體轉染細胞及正常細胞無此現象;細胞裂解物sds - page電泳結果顯示:只有重組質粒轉染的細胞在約38kd處有明顯的蛋白帶,這與理論計算的ts87基因表達蛋白的分子量為38kd基本一致; western - blot分析結果顯示:約38kd的蛋白帶能夠分別被旋毛蟲感染兔血清,成蟲蟲體可溶性抗原免疫兔血清, ts87基因原核表達蛋白免疫兔血清( ts87血清)以及一株具保護性的旋毛蟲單抗特異識別。Epigenetic modification of the genome ensures proper gene activation during development and involves : genome methylation changes ; the assembly of histon and histone variants into nucleosomes and remodeling of other chromatin - associated proteins such as linker histones, polycomb group, nuclear scaffold protein, and transcription factors
這些染色質的再程序化包括dna的甲基化和核小體組蛋白的乙酰化等。組蛋白乙酰化在染色質結構調節、 dna復制、基因轉錄、細胞分化及癌細胞發育都有研究,但在克隆動物中卻是一個有待深入探索的領域。Telomerase is a ribonucleoprotein complex ( rnp ) composed by its rna component and protein subunits. telomerase can synthesize telomeric dna onto chromosomal ends using its own rna component as a template, elongate the length of telomere, increase cell life and even induce cell immortalization
端粒酶( telomerase )是由端粒酶rna和蛋白質組成的一種核糖核蛋白復合物( rnp ) 。端粒酶含有引物特異識別位點,能以自身rna為模板,逆轉錄合成端粒dna並加到染色體末端,使端粒延長,從而延長細胞的壽命甚至使其永生化。In this paper, the effect of nuclear actin on the process of chromosome construction has been studied by utilizing the precise natural synchrous plasmodium of physarum polycephalum, sds - polyacryl amide gel electrophoresis ( sds - page ), western blotting, the cell - free system and optics microscopy. the major results and conclusions are as follows : 1
本實驗以多頭絨泡菌原質團為材料,採用同步化培養、細胞核提取、 sds - page 、免疫印跡、非細胞體系構建、光學顯微鏡觀察等方法,研究了有絲分裂前期核內肌動蛋白對染色體構建的影響。Tissue sections from every animal were double - labeled with the antibodies of map - 2, cox - 2, gdnf, caspase - 3 and either the neuron - specific antibody neuronal nuclear protein ( neun ) or the astroglial - specific marker glial fibrillary acidic protein ( gfap ). we carried out a series of research to explore the effects and mechanism of map - 2, cox - 2, gdnf, caspase - 3 during tbi and trie d to provide some useful theory basis for both the treatment of tbi in the practice and forensic medicine
並通過上述指標分別與神經元特異性標志物神經元核蛋白( neuronalnuclearprotein , neun )和星形膠質細胞特異標志物膠質纖維酸性蛋白( glialfibrillaryacidicprotein , gfap )進行免疫組織化學雙染色,探討腦損傷后神經元及神經膠質細胞反應性變化情況及其分子生物學機制,以期為腦損傷研究提供有益的數據材料,也為以上指標在法醫學實際檢案的應用提供必需理論依據。In order to avoid the possible contamination of the cytosolic actin, we ultilized a cell - free system to study the effect of nuclear actin on the process of chromosome construction
細胞核中的肌動蛋白可能參與了核內的一些重要的生理活動,如核骨架維持、染色質集縮和rna轉錄等。Cultured epithelial cell undergoing division. this cell is in prophase of mitosis. microtubules are shown in green, actin is in red and mitotic chromosomes are colored blue
以上兩圖為正處于有絲分裂前期的動物上皮細胞。綠色的是微絲,紅色為肌動蛋白,染色質高度螺旋成為粗短的染色體(藍色) ,核膜核仁逐漸解體,染色體不規則地分佈於細胞內。This study we acquired the coding region of hcv ns5b gene by pcr of hcv full length genome and construct the recombinant plasmid pegep n3 - ns5b ; with the different concentration of g418 in the culture medium, we think the selection concentration of g418 for hepg2 cell is 800 g / ml ; the recombinant plasmid was transfected into hepg2 cell by lipofectamine2000 cells containing stable transformants were selected by the ability of resistance to g418 and isolated with the limited dilution. the stably transfected cell line expressing ns5b - egfp fusion protein was obtained by the detection under fluorescence microscope and rt - pcr
本研究首先從hcv基因組中擴增出nssb基因,構建了nssb基因與報告分子egfp (增強型綠色熒光蛋白)基因的融合基因真核表達質粒pegfpn30 ;通過含有不同g418濃度梯度的培養液培養hepgz細胞,確定了篩選用g4181作濃度為800pg ml ;利用脂質體法將該重組質粒轉染hepgz細胞,經過有限稀釋法和g4壓力選擇,應用熒光顯微鏡和rtpcr檢測,獲得可穩定表達nssbegfp融合蛋白的hepgz細胞克隆。分享友人