染色體外因子 的英文怎麼說
中文拼音 [rǎnshǎitǐwàiyīnzi]
染色體外因子
英文
extrachromosomal element- 染 : Ⅰ動詞1 (用染料著色)dye 2 (感染) catch [contract] (a disease) 3 (沾染) acquire (a bad hab...
- 色 : 色名詞[口語] (顏色) colour
- 體 : 體構詞成分。
- 外 : Ⅰ名詞1 (外面) outside; external side 2 (外國) foreign country 3 (以外) besides; beyond; in ...
- 因 : Ⅰ動詞[書面語] (沿襲) follow; carry on Ⅱ介詞1 [書面語] (憑借; 根據) on the basis of; in accord...
- 子 : 子Ⅰ名詞1 (兒子) son 2 (人的通稱) person 3 (古代特指有學問的男人) ancient title of respect f...
- 染色體 : [生物學] chromosome染色體疾病 chromosomal disorders; 染色體異常 chromosome abnormality
- 染色 : dye; dyeing; colouration; tintage; tinging; dyschroia; colouring; colour; [半] decoration染色不足...
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First, to construct a recombinant plasmid pegfp - c - fos with c - fos promoter and egfp, and then transfect it into human bladder transitional cell carcinoma biu - 87 cell ; second, based on the changes of the expression of gfp in the biu - 87 cell which induced by the aconitine and hab toxins, the concentration of the hab toxins could be detected
目的:構建一個含c - fos啟動子和egfp報告基因的pegfp - c - fos重組質粒載體。體外轉染膀胱癌biu - 87細胞后,利用赤潮毒素作用后細胞表達綠色熒光蛋白的變化來檢測赤潮毒素,初步建立一種以細胞為基礎受體水平的赤潮毒素檢測方法。Human gnt - v contains 741 amino acids with six potential sites for n - glycosylation and bears high homology to gnt - v of rat. its gene is located on chromosome 2q21 containing 17 exons. gnt - v protein is encoded by exons 2 - 17 as open reading frame
人類gnt - v由741個氨基酸組成,有6個潛在的n -糖基化位點,基因定位於染色體2q21 ,含有17個外顯子,其開放閱讀框架由外顯子2 - 17進行編碼。It is due to the lack of homologous pairs of chromosomes, so chromosomes cannot pair in meiosis, and the resulting gametes may have extra or missing chromosomes
這歸因於染色體同系對的缺乏,以至於在減數分裂中染色體不能配對,導致配子有可能有額外或缺失染色體。The e2 genes above of the prevalent strain ( guangxi yulin strain ) were cloned respectively into secreted expression vector ppic9k of eukaryotic expression system p. pastoris and transformed into p. pastoris by electroporation after linearization, 25 high - copied transformants were obtained by g418 screening. it was proved that the e2 genes were integrated stably into chromosome of p. pastoris by dot blot and dna sequencing
豬瘟病毒e2基因的真核表達:分別將csfv兩個代表株的e2基因克隆入畢赤酵母( p . pastoris )分泌型表達載體ppic9k中,酶切線型化后電穿孔導入p . pastotis進行整合,經g418篩選得到25個高拷貝轉化子,經dna斑點試驗和dna測序證明外源基因e2穩定地整合到p . pastoris染色體中。In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection
此外,為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況,我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中,與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒,將其感染sf9細胞制備p1種子液,同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定,確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。Additionally, hau3r gene with it own promoter was cloned into high - copy plasmid pij653 and integrative plasmid pset152, respectively. transformants of s. lividans zx1 carrying these clones were infected with hau3, respectively, but the results shown that there was no significant correlation between the copy number of hau3r gene and the level of resistance to hau3
另外,將染色體來源的攜帶自身啟動子的hau3 ~ r基因分別克隆到高拷貝和低拷貝載體上並將其導入變鉛青鏈黴菌zx1 ,在實驗條件下未發現拷貝數與抗性水平間存在顯著相關性。The researchers described seen genetic risk factors ? dna sequences present in some people but not others ? bunched in a relatiely small region of one of the human chromosomes, chromosome 8, that reliably predicted one ' s probability of deeloping prostate cancer
研究者們介紹了這七個遺傳風險因子一些人所含有的特殊的dna序列而另外的人卻沒有聚集在人類8號染色體上一個相對較小的區域,而且它們能夠可靠的預測一個人患前列腺癌的可能性。Ii. results of study on tyrp1 and id : ( 1 ) we first sequenced the 10, 066 bp whole genomic sequences of chicken tyrp1. analysis of the sequences indicates that : compared with human tyrp1, chicken tyrp1 is short of an intron and there is two microsatellites in introns
(二)本文對z染色體上與黑色素相關的基因tyrp1和id的研究結果如下: ( 1 )獲得了10 , 066bp的tyrp1的全長基因組序列,分析結果表明:絲羽烏骨雞的tyrp1外顯子與人的tyrp1外顯子同源性為70 . 1 。分享友人