柱層析法 的英文怎麼說
中文拼音 [zhùcéngxīfǎ]
柱層析法
英文
column chromatography-
After the protein refolding of denaturant inclusion body following dialysis, we got the pure recombinant gst - eo protein by gst affinity columns. using the purified protein as coating antigen, an indirect elisa were developed for detecting the anti - eo antibody in the csfv serum by exploring the concentration of coating an tigen and dilution degree of serum
使用分步透析法對變性的包涵體進行復性，將復性蛋白過gst親和層析柱得到純化的gst - eo融合蛋白。以gst - eo融合蛋白為診斷抗原，初步建立了用間接elisa檢測豬瘟血清eo抗體的方法。Fourthly, according to the activity, collect, dialyse, freeze, dry respectively the sod protein through deae - sepharose column chromatog - raphy ; process the sod protein through sephacryl s - 200 column chromatography with the preceding method. at last, process the pure sod protein with same functional enzyme electrophoresis and active dye, isoelectric focusing electrophoresis and sds - page
將粗酶液過deae -瓊脂糖柱層析，得三個活力峰，分別收集、透析、乾燥濃縮后；再上sephacryls - 200凝膠柱層析，按與deae -瓊脂糖柱層析后同樣的方法收集處理。This enzyme was different with the ones reported in the past. a phosphatase was isolated from the chloroplast thylakoid membrane of ipomoea aquatica, by nacl extration, ammonium sulfate precipitation, ion - exchange chromatography and hydrophic chromatography through butyl - toyopearl 650m column
使用nacl抽提、硫酸銨分步沉澱、離子交換和butyl - toyopearl650m疏水柱層析等方法，從蕹菜葉綠體類囊體膜中分離純化到一種蛋白磷酸酯酶。Analysis of honey - determination of the content of streptomycin - high performance liquid chromatography postcolumn derivatisation
蜂蜜分析.鏈酶素含量測定.高效液相色層支柱衍生法Methylbenzyl amine as chiral auxiliaries to react with racimic ketorolac. then authors got optical amides. the diastereoisomeric mixture were separated by column - chroatography on silica gel. the results of separation depend on the structure of r
苯乙胺作為手性輔基，與外消旋酮咯酸生成一對光學活性的酰胺衍生物，再用簡單易行的柱層析方法分離其非對映異構體。- acetolactate decarboxylase is purifed from cell extract by 50 % - 80 % ammonium sulfate - fractionation, 50, 2min heat treatment and deae - sepharose fast flow column chromatography, which we study the different ph and different buffer of deae - sepharose fast flow column chromatography and conclude ph 6
對其酶學性質進行了研究。 -乙酰乳酸脫羧酶經50 80硫酸銨分級沉澱、 50 ， 2min熱處理、 deae - sepharosefastflow離子交換柱層析方法分離純化。Prestressed concrete slab - column structure has been widely used in long - span and multistory building. there are four commonly used analysis methods for slab - column structure, which are direct design method, equivalent frame method, plasticity method and finite element method
預應力混凝土板柱結構在大開間的多層及高層建築中得到了廣泛的應用，其設計方法有直接設計法、等代框架法、塑性分析法和有限元法等多種方法。This article deduced the calculation method of column hinge sway in framework structure by analyzing plastic sway of the column hinge sway mechanism ? the most dangerous collapse model in the framework structure with the virtual principle of plastic analysis, and calculated two framework structures and the results is almost the same as those calculated by method of plastic analysis
通過對框架結構最危險的倒塌模型? ?柱鉸形側移機構，對薄弱層的塑性變形通過塑性分析的虛功原理推求框架結構柱鉸形側移的計算方法，應用此計算方法對大連市兩棟框架結構進行計算，其結果與彈塑性時程分析法結果基本相符。The bioactive strain ' s fermentation product was isolated and purified primarily using methods of solvent extraction, acid - alkali extraction, ab - 8 macropore absorption chromatography, 1 x 007 positive ion exchange chromatography, the result showed the purification product has prominent bioactivity inhibit staphalococcus aureus
中國熱帶農業科學院、華南熱帶農業大學2003屆碩士研究生採用有機劑抽提法、酸堿抽提法、大孔吸附樹脂柱層析和陽離子交換樹脂柱層析等方法對hsl 306菌株發酵產物進行了初步分離純化。The best method for isolation and purification of - dihydroagarofuran sesquiterpene in celastrus angulatus is alternate normal - phase and reserve - phase high performance liquid chromatography
結果表明，對苦皮藤中富含二氫沉香呋喃倍半萜類化合物部分進行分離的過程中，採用正相、反相高壓制備柱層析交替進行的方法最為有效。Methods the oil in the seed of panax ginseng c. a. mey was extracted by silica column chromatography, and were analyzed by gc - ms
方法採用硅膠柱層析法分離出林下參種子油，應用gc - ms技術進行成分分析。Method the large defects of articular cartilage were repaired with grafts of freeze - stored and fresh chondrocytes cultured on fascia, periosteum and articular cartilage, which were evaluated by a number of observation methods
方法用凍存和新鮮的異體筋膜上培養的軟骨細胞、骨膜和關節軟骨移植修復大面積關節軟骨缺損，通過大體標本、光學顯微鏡、掃描和透射電鏡、放射自顯影、微量元素和柱層析氨基酸定量測定、一氧化氮含量測定等多種觀察方法進行評價。Under the horizontal earthquake action and wind force, aim at " the pure frame structure with rectangle columns ", " the pure frame structure with special - shaped columns ", " the frame - truss structure with special - shaped columns " and " frame - shear wall structure with special - shaped columns ", earthquake action analysis was done by the spacial finite element method through the changes structural parameter. analyzing systematically " structure vibration mode ", " vibration period ", " structure lateral rigidity ", " seismic action force ", " seismic response force ", " floor seismic shear force ", " lateral horizontal displacement of structure " and " members internal force ". results indicate : ( 1 ) based on equal area, the special - shaped columns replaced the rectangle columns, the structural lateral rigidity enlarges, the lateral displacement minishes obviously, the earthquake response increase slightly, the biggest increasing amount of frame columns axis - compress ratio is smaller than 0. 08. the whole aseismic performance of structure has improved ; ( 2 ) when the section ' s length and section ' s thickness ratio of special - shaped columns is smaller than 3. 6, the structure benefits to resist seismic action ; ( 3 ) the angle of horizontal seismic action with the whole coordinate is 0 degree, structure earthquake response is bigger, belonging to a control factor of structure aseismic design ; ( 4 ) the frame - truss structure with special - shaped columns and the " a " - brace has the biggest lateral rigidity ; ( 5 ) the frame - shear wall structure with special - shaped columns have bigger lateral rigidity and smaller displacement, members internal force enlarged just rightly, have much superiority of resisting seismic action ; ( 6 ) in the higher seismic fortification criterion region ( 8 degree of seismic fortification intensity ), aseismic disadvantageous building site ( iii type site ), adopting special - shaped columns structure system, should reduce possibly the building ' s self - weight in order to reduce the earthquake response ; ( 7 ) the response spectrum method of computing seismic response and the time - history analysis method have similar analysis result
西安理工大學碩士學位論文在水平地震作用下，並考慮風荷載組合，分別對「矩形柱純框架結構」 、 「異型柱純框架結構」 、 「異型柱框一析架結構」及「異型柱框-剪結構」等四種結構體系，通過改變結構參數，運用空間有限元方法，進行地震作用計算。系統地分析研究「結構振型」 、 「振動周期」 、 「結構側向剛度」 、 「結構地震反應力」 、 「樓層地震剪力」 、 「結構側向位移」 、 「層間位移角」 ，以及「構件內力」 。結果表明:在等面積原則下，異型柱代換矩形柱后，結構剛度增大，側向位移明顯減小，地震反應力略有增加，框架柱軸壓比最大增幅小於8 % ，結構整體抗震性能有所提高;當異型柱肢長肢厚比小於等於3 . 6時，結構有利於抗震;水平地震作用力與整體坐標夾角為0度時，結構地震作用效應較大，屬結構設計的控制因素之一;異型柱框一析架結構採用「人」字斜撐，側向剛度大於「八」字斜撐和「人一八」字混合斜撐;異型柱框一剪結構側向剛度大、位移小，構件內力增大適中，是一種抗震性能優越的結構體系:在抗震設防烈度較高地區（ 8度）和抗震不利的建築場地（ m類場地） ，採用異型柱結構體系時，應盡可能減輕結構自重，降低地震作用力;分別採用「振型分解反應譜」法和「時程分析」法進行地震作用計算，兩種方法所得結果基本一致。The ascites fluid antibody purified by 33 % ammonium sulfate is further purified by chromatography. the p24 affinity chromato - graphic column done to purify the bispecific antibody is in the brcn activated sepharose 4b. after washing the unspecific proteins, change the washing buffer and the anti - p24 / anti - human rbc type a bispecific antibody and the anti - p24 monospecific antibody are gained respectively
方法是先用硫酸綏鹽析，再過親和層析柱，即33飽和硫酸按沉澱后，透祈過夜，上p24抗原做配體的濱化氰活化的sepharose4b柱，流洗掉雜蛋白和抗人紅細胞單特異性抗體后，再洗脫並收集雙特異性抗體。In this paper, we extracted the antioxidative substance from alpinia oxyphylla miquel by using column chromatography
摘要探討了干柱層析法分離益智渣及益智乙醇提取物的抗氧化成分。4m phosphate buffer ; the second is that the crude enzyme runs on sephadex g100 - g50 column under the same experimental conditions ( only with sephadex g100, g50 ). at last polyacrylamide gel electrophoresis and sod assay tell us both of products are highly purified sod
因而不管從第一次的純化來看，還是從文獻上報道的來看，本實驗的串聯柱層析是較為合理有效的一種生產方法，值得推廣。The crude extraction of dry pea seed, which was obtained through marination, homogenization, filtration, centrifugation and other methods, was precipitated by 50 mmol / l ( final concentration ) mgq2. the pellet was chromatographed on aca ^ gel filtration and deae - cellulose 52 ( de 52 ) anion - exchanger. single eluting peak containing ferritin was obtained finally
2 、豌豆種子鐵蛋白的純化豌豆種子經浸泡、勻漿、過濾、離心等操作得到豌豆鐵蛋白粗提液，再用終濃度為50mmol / l的mgcl _ 2鹽析等處理后，經aca _ （ 22 ）凝膠過濾柱層析和deae -纖維素52 （ de52 ）陰離子交換柱層析等方法進行純化，得到單一的豌豆鐵蛋白洗脫峰。Seperate method of chrysophanol and physcion in dry column chromatography
干柱層析法分離大黃酚和大黃素甲醚On the premise of summarizing former contributions in the field, and considering the universality of the cylindrical space with circular cross - section in industry, the author develop a novel ofpt structure, on which the design theories, numerical simulation, design of experimental system for plane structure, preliminary experiment and design of applied space structure are presented. the main contents of the thesis are that : put forward the novel ofpt pixel distribution and plane - light - path design theories and their empirical formulae. the numbers of the ofs units and their emitting rays can be decided by the requirements of image - reconstructed resolution and the size of the center unmeasured region
本論文在總結前人工作的前提下，充分兼顧了工業中具有圓形截面的圓柱形容器或管道普遍存在的事實，提出了一種新型的光纖過程層析成像結構，並從理論設計方法，具體結構模擬，平面結構實驗系統設計，初步實驗以及空間結構實用化設計、理論分析等方面進行了研究，主要的創新點在於：提出了一種用於工業過程監測與控制的新型光纖過程層析成像結構設計方法，並推導了該方法的經驗公式，該方法主要包括像素分配和平面光路結構設計，可以根據工程應用中圖像重建的解析度和圓形截面非測量區域所佔比例的要求，設計所需傳感單元的數目和傳感單元發射光線的數目；設計了一種新型的光纖過程層析成像結構，並對該結構進行了可行性分析和數值模擬。It is proved that the method of solvent extraction - column chromatographic separation is a good method for the production of high value chemicals, such as phenols, from bio - oi1
實驗也證明萃取與柱層析相結合是分離制取酚類化工產品的很好方法。分享友人