栝樓子 的英文怎麼說
中文拼音 [guālóuzi]
栝樓子
英文
semen trichosanthis kirilowii-
The transformed hairy roots were detected for agropine to make sure the transformation was successful. a conclusion was reached as follows : agrobacterium rhizogenes r1601 was an ideal strain for hairy roots transformation from trichosanthes bracteata voigt which exhibited extraordinary transformation rate ( 60 % ). od600 = 0. 7 was the optimum infection concentration for the tested transforming agent, with temperature at 25, ph at 6. 0, 20min of infection and 48h of co - culture and phytohormone at 1. 0mg / l being the most favourable hairy root - inducting pattern
通過實驗和對結果的數據分析，得出下面的結論：發根農桿菌r1601是較好的大苞栝樓發根的誘導菌株，它對預培養24h的大苞栝樓子葉外植體具有最好的轉化效率和發根誘導率（ 60 ） ，最適的菌株感染濃度od _ （ 600 ）為0 . 7 ，溫度27 、 ph值為6 . 0 、 20min的感染時間和48h的共培養，以及黑暗條件和1 . 0mg / l的激素最有利於發根誘導的環境條件。Aimed at developing a set of methods efficient in inducing the hairy roots with agrobacterium rhizogenes from trichosanthes bracteata voigt to meet needs for hairy roots for bioreactor culture research and application ; and also in order to investigate the roles of explants, agrobacterium rhizogenes, affecting factors and their magnitude and the underlying mechanism in the transformation, therefore to determine the optimum explants, pre - culture time, tested strain concentration, infection time, co - culture time, affecting factors and their effects working together and to obtain the optimum transforming condition by performing orthogonal test, this project was launched. with cotyledons and stems from trichosanthes bracteata voigt plantlets as explants, agrobacterium rhizogenes r1601 and r1000 as infection agents, transformation was performed using different methods and under various circumstances
為了建立一種有效的誘導大苞栝樓發根的方法及其發根培養體系，以適合於發根生物反應器擴大培養研究和應用基礎的需要；本研究對誘導發根的外植體在發根農桿菌和環境因子等影響作用下進行發根的誘發及其可能的機理進行探討，從而找出用於發根誘導和培養的最佳外植體、預培養時間、發根農桿菌濃度、感染時間和共培養時間、誘導條件，並通過正交試驗得出優化的誘導條件。
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