核內受體 的英文怎麼說
中文拼音 [hénèishòutǐ]
核內受體
英文
intranuclear receptor-
Translocation from endocytic compartments to the cytosol is the essential and rate - limiting step in the intoxication process of most toxins such as ricin, diphtheria toxin, shiga toxin and pseudomonas exotoxin ( pe ). a number of these toxins are transported to trans - golgi network ( tgn ), and in many cases such transport to the tgn is required for the translocation and cytotoxicity. in deed, 5 % of the ricin endocytosed by cells has been shown to reach the tgn
蓖麻毒素進入細胞的機理不甚明了,一般認為是rtb先與細胞膜受體結合,主要經過受體介導的內吞作用進入吞噬體,然後沿著內體、高爾基體、內質網等逆向分泌途徑,有序地運輸到內質網,最後從內質網轉位進入細胞漿,在胞漿內攻擊核糖體,從而抑制蛋白質的合成,導致細胞死亡。Injected group, 0. 1 % saccharin ( 1. 5 ~ 2ml / rat, in 5min ) intraoral infused group and cta group. the expression of endogenous leucin - enkephalin ( lek ) in the rat brain was observed and 5 parts of the thalamus including laterodorsal thalamic nucleus ( ld ), lateral part of mediodorsal thalamic nucleus ( mdl ), ventroposterolateral thalamic nucleus ( vpl ), ventroposteromedial thalamic nucleus ( vpm ) and reticular thalamic nucleus ( rt ) were comparatively researched before and after the acquisition of cta applying lek - immunocytochemistry. in behavioral experiment, 18 adult male sd rats were divided into normal cta group ( control ) and 2 naloxone i. p
為探討cta形成過程中enk的作用,本實驗用成年雄性sd大鼠35隻,分為空白對照組、生理鹽水( 2體重)腹腔注射組、 0 . 15mlicl溶液( 2體重)腹腔注射組、 0 . 1糖精溶液口腔灌流組( 1 . 5 - 2ml只, 5min )和cta建立組,採用免疫細胞化學方法,觀察了亮腦啡肽( lek )陽性神經元在大鼠腦內的分佈情況,並比較了各組大鼠丘腦外側背核( ld ) 、丘腦內側背核外側部( mdl ) 、丘腦腹后外側核( vpl ) 、丘腦腹后內側核( vpm )以及丘腦網狀核( rt )等5個腦區內lek表達水平的差異;另外將成年雄性sd大鼠18隻,分為正常cta建立組以及在cta建立前或cta建立后阿片受體拮抗劑納洛酮( 2mg kg體重)腹腔注射組,對內源性阿片樣物質對于cta建立和保持的影響進行了行為學研究。Background : ghrelin is a 28 - amino acid endogenous peptide recently identified in the secretory granules of x / a - like cells in the rat stomach, which can act on the growth hormone secretagogue receptor to accelerate the secretion of growth hormone. it was identified that ghrelin - immunoreactive cell and its receptor localize in areas of brain, such as hypothalamus ( pvn, arc ), hippocampus, adenohypophysis and so on. motilin is a 22 - amino acid peptide secreted from the upper part of the small intestine, which regulates the interdigestive motility of gastric
背景資料: ghrelin是新發現的由28個氨基酸組成的內源性腦腸肽,由胃部泌酸腺x / a樣內分泌細胞分泌, ghrelin免疫活性神經元及其受體在中樞神經系統中如下丘腦室旁核( paraventricularneucleus , pvn ) 、弓狀核( arcuatenucleus , arc ) 、海馬區及腺垂體等均有不同濃度的分佈,可與生長素促泌物受體( growthhormonesecretagoguereceptor , ghsr )結合促進生長素( growthhormone , gh )分泌。Thus, studies on change of inflammatory cytokines receptors expressions in the brains of immune model animals have important significance for revealing the nuclei and cells participating neuroimmunomodulation
因而,觀察免疫模型動物腦內炎性細胞因子受體表達的變化對揭示腦內參與免疫調控的核團和細胞具有重要的意義。The present results indicated that the paraventricular nucleus of the hypothalamus and the supraoptic nucleus might have important roles in neuroimmunomodulation. 2. following lps or seb was administered intraperitoneally, the expression of pcna of splenic cells and il - 1 receptor type i in pvn and son were observed by using immunocytochemistry in the mice. double fluorescent labeling technique was used to determine the relationship of il - 1 receptor type i co - expressions with arginine vasopressin or oxytocin
二、小鼠腹腔內給予細菌內毒素lps或腸毒素seb ,用免疫組織化學方法觀察了脾臟核增殖抗體及下丘腦室旁核和視上核中1型il 1受體的表達,並採用雙標記技術觀察了1型il刁受體陽性神經元和加壓素及催產素表達的關系。It is toxic for most mammalian cells since ricin a chain ( rta ) is an rna specific n - glycosidase that removes a specific adenine residue in a highly conservative region from among over 4000 nucloside residues present in 28s rrna, and causes protein synthesis inhibition and cell death
Rta具有n -糖苷酶活性,可催化28srrna在4234位脫去腺嘌呤,使核糖體60s亞基失活,從而抑制蛋白質合成,導致細胞死亡。 b鏈( rtb )是結合鏈,能和細胞表面半乳糖受體結合,協助a鏈進入細胞內。Electrophysiological experiment : in 73 rats, extracellular recordings in vivo were made from pvn using 3 - barrel microelectrode. neurons were categorized as gastric distension excitatory ( gd - e ) or inhibitory ( gd - i ) neurons tested with gastric distension stimulus. drugs were applied through the 3 - barrel microelectrode by a 4 - programmable pressure injector ( pm2000b, mdi, usa ) : relin, saline ( control group ) relin, " [ d - lys - 3 ] - ghrp - 6 ( antagonist for ghrelin - r ), to observe the effects of drugs on the neuron discharge
電生理實驗方法:在73隻大鼠中,應用三管玻璃微電極細胞外記錄麻醉大鼠一側pvn神經元自發放電,用水囊充盈胃鑒別胃擴張敏感神經元( gdsn ) ,以壓力注射儀( pm2000b , mdi , usa )經三管玻璃微電極,對核團內中文摘要單個神經元分別微量注射給予: ghrelin 、生理鹽水ns (對照) ghrelin 、 d一lys一3 ]一ghrp一6 ( ghrelin受體拮抗劑) ,觀察藥物對神經元單位放電的影響。The results showed that the expressions of il - 1 receptor type 6 tt i in normal adult rat brain were very widely, and there were intense immuno - positive cells of il - 1 receptor type i in septal area, medial preoptic area, neocortex, hippocampus, paraventricular nucleus of the hypothalamus, supraoptic nucleus, hypothalamic ventromedial nucleus, arcuate nucleus and median eminence
結果顯示:型il - 1受體在正常成年大鼠腦內有廣泛的表達,隔區、視前內側區、新皮質、海馬、下丘腦室旁核、視上核、下丘腦腹內側核、弓狀核和正中隆起等部位有較多型il - 1受體陽性的細胞。Some transgenic plants harboring the gfp tnos vector also exhibited inhibited expression of gfp, and northern blots also revealed that most gfp mrnas transcribed from tnos accumulated in the nucleus and was not transported into the cytoplasm
獲得了含有gfp - tnos表達載體的轉基因本生煙,在部分轉基因株系中gfp基因的表達受到抑制。 northern分析顯示: gfp - tnos載體所表達的gfp基因產物多數滯留在細胞核內部,不能被運送到細胞質中。They were coinjected into the male prenuclei of fertilized eggs with hsa. dna together. after that normal injected eggs were selected and transferred to the oviduct of pseudopregment recipents mice and gave birth to 65 fl offsprings, the foreign genes were found integrated in 12 of 65 mice by pcr and southern blotting detection
用顯微注射法分批把這三種不同的dna片段導人小鼠受精卵雄原核,並移植入假孕受體鼠,產生的65隻n代小鼠中,經pcr和southern雜交檢測,表明轉入的基因在12隻小鼠體內有整合段。Conclusion the results indicated that neurons with nig receptor - li were widely distributed in the central nervous system suggesting that it may be involved in many physiological functions in the mouse central nervous system
在高滲鹽水刺激的條件下,室旁核中大部分n 』 k3受體樣陽性神經元可表達fos 。結論n 』 k3受體廣泛分佈於小鼠中樞神經系統內提示它可能具有重要的生理功能。C - kit is a receptor tyrosine protein kinase on the cellular membrane, while c - myc is a transcription regulatory factor in the nucleus
Kit是位於細胞膜上的受體酪氨酸蛋白激酶, myc是位於細胞核內的轉錄調節因子。Its gene includes seven exons ( 3 - 9 ) and six introns ( 3 - 8 ). with respect to zfghrl, it is also like other ghr1s, which consists of 1578bp and encode a 528 amino acid protein. its gene only includes eight exons ( 1 - 5, 8 ) and seven introns ( l - 5 ). various methods used to find out the transmembrane and boxl domain of zfghrl but the result turns out to be fruitless due to unknown reason. fghr2 and zfghr2 : fghr2 includes eight exons and seven introns, which consists of 1710 bp and encodes a 569 amino acid protein ; zfghr2 includes eight exons ( 1 - 5, 8 ) and seven introns ( 1 - 5 ), which consists of 1758 bp and encodes a 484 amino acid protein
得到的fghr1含有7個外顯子( 3 - 9 )和6個內含子( 3 - 8 ) ,由1755個bp的核苷酸組成並編碼一個584個氨基酸殘基的蛋白質:得到的zfghr1包含有1587個核苷酸並編碼一個528個氨基酸殘基的蛋白質,理學碩士學位論文:南方貼生長激素受體cdna的分子克隆和魚類存在兩種生長激素受體的證明其基因只有8個外顯子( l一5 , 8 )和7個內含子( 1一5 ) 。In order to know if the genetic constitution of recipient oocytes influenced the development of embryos reconstructed from es cell, oocytes of c57bl / 6j and kunming albino were used as recipient, respectively
為了了解受體卵的遺傳內涵是否對胚胎幹細胞重構胚發育也會產生影響,分別以c57bl 6j和昆明白小鼠的m期去核卵為受體,構建胚胎幹細胞重構胚。To make clear the hypothesis, a middle cerebral artery occlusion ( mcao ) and hypoxia and glucose - deprivation ( hgd ) ischemic models were used in in vivo and in vitro study, respectively. we first studied the cellular localization of kvl. 2 and the co - localization of kvl. 2 protein and vegf receptors flk - 1 and flt - 1, observed the effect of mcao on kvl. 2 expression and phosphrylation in the rat brain in vivo, then investigated the effect of vegf on ischemia / hypoxia cell damage and tyrosine phosphorylation of kvl. 2 in sh - sy5y cells. finally, in order to further elucidate the relationship between vegf ' s neuroprotection and its regulation on kvl. 2 phosphorylation, we used a specific antisense oligodeoxynucleotide ( odn ) to knockdown the expression of endogenous vegf to observe its role in ischemia / hypoxia cell damage and regulation of kvl. 2 phosphorylation
為了驗證上述假設,本文分別在整體和離體水平,採用大腦中動脈缺血( middlecerebralarteryocclusion , mcao )和體外氧?糖剝奪( hypoxiaandglucose - deprivation , hgd )缺血模型,首先了解了kv1 . 2蛋白的細胞定位及與vegf受體flk - 1和flt - 1的共存情況,觀察了整體mcao后缺血再灌不同時間大鼠腦內kv1 . 2蛋白的磷酸化水平變化,然後通過外源性給予vegf蛋白,在sh - sy5y細胞株上觀察其對缺血細胞存活率及kv1 . 2蛋白磷酸化水平的影響,最後利用vegf反義脫氧寡核苷酸( oligodeoxynucleotide , odn )特異阻斷內源性vegf蛋白的表達,觀察內源性vegf蛋白在缺血細胞損傷及調節kv1 . 2蛋白磷酸化中的作用,以進一步明確vegf缺血保護效應與其調節kv1 . 2蛋白磷酸化之間的關系。In the first part, we observed the changes of expressions of type i receptor of il - 1 in the rat and mouse brain after intraperitoneally administration of different kinds and doses antigens respectively. in the second part including two experiments we cloned rat il - 6r ' s genes by pcr, expressed them in e. coli dh5 a and cos - 7 cell, and produced il - 6r ' s polyclonal antibody which is proved having more high liter, affinity and specificity 1
第一部分採用不同種類和劑量的抗原刺激,分兩個實驗,觀察了大、小鼠腦內il - 1的i型受體表達的變化,探討了腦內參與免疫調控的核團和細胞類型;第二部分分兩個實驗,運用pcr技術克隆了大鼠il - 6r的基因並進行了原核和真核表達,制備了特異性高、親和力強和較高效價的抗il - 6r多克隆抗體,為進一步進行il - 6r的研究奠定了基礎。This study were designed to investigate development potency of the reconstructed embryos of black bear - cat inter - species nuclear transfer in vitro / vivo, to develop the principle and technology of somatic nuclear transplantation in inter - species of mammalian and to study the mechanism of coordination between cell nuclear and cytoplasm. somatic nuclear transplantation technology has been used to establish stem cell from different animals, which can provide materials to treat various diseases
本研究運用體細胞核移植的原理和技術,以六月齡雌性黑熊的成纖維細胞為供核體,去核未受精家貓成熟卵母細胞為受體,構建種間核移植重組胚胎,研究種問體細胞核移植胚胎的構建方法和重組胚胎的體內外發育潛能及其影響因素。This study were designed to investigate development potency of the reconstructed embryos of rat - mouse and rat - bovine inter - species nuclear transfer in vitro / vivo, to develop the principle and technology of somatic nuclear transplantation in inter - species of mammalian and to study the mechanism of coordination between cell nuclear and cytoplasm. somatic nuclear transplantation technology has been used to establish stem cell from different animals, which can provide material to treat various diseases
本研究運用體細胞核移植的原理和技術,以成年大鼠的成纖維細胞為供體,去核小鼠和牛成熟卵母細胞為受體,構建種間核移植重組胚胎,研究種間體細胞核移植胚胎的構建方法和重組胚胎的體內外發育潛能及其影響因素。The expressions of il - 1 receptor type i in mouse brain were widely distributed. and the expressions in mouse pvn and son were significantly enhanced by an intraperitoneal administration of lps or seb ( p < 0. 05 or 0. 01 ). the arginine vasopressin - and oxytocin - containing neurons in the pvn and son also displayed immunofluo - rescence labeling for il - 1 receptor type i
F0刀1 ) ; i型il 1受體在正常小鼠腦內的表達很廣泛,與對照組比較, lps或seb引起1型幾l受體在小鼠下丘腦室旁核和視上核中表達顯著增強( oIncyte does not actually own the rights to the gene for the histamine receptor in your body but only to an “ isolated and purified ” form of it. ( at times, patent examiners or courts have invoked the u. s
早期的基因專利還依循傳統化學專利的模式,例如因賽特公司並不是真的擁有你體內的組織胺受體基因,而是分離和純化形式的基因(當時專利審核人員或法院還引用了美國憲法中禁止奴役的條文,解釋為什麼不能申請一個真正的人或其身體部位的專利權) 。分享友人