核苷酸殘基 的英文怎麼說
中文拼音 [hésuāncánjī]
核苷酸殘基
英文
nucleotide residue-
In this research two full - length cdnas have been cloned by a combination of rt - pcr and 3 " - is1 - race with synthesized degenerate primers from young leaves of vicia faba l., pichia methanolica high - level expression systems of the genes have been constructed, and the milligram expressed protein was purified using probond resin purification system, which may result in further identification of the function of the aba binding protein. the full - length cdna of abp370 fragment is 3449 bp long and has an open reading frame of 2304 bp encoding 768 amino acids with 876 bp long 5 ' - utr, 369 bp long 3 ' - utr and poly ( a ) tail. the full - length cdna of abp640 fragment is 1012 bp long and has an open reading frame of 780 bp encoding 260 amino acids with 88 bp long 5 ' - utr, 144 bp long 3 " - utr and poly ( a ) tail
3 - 5 - race擴增片段序列分析結果表明， abp370擴增片段的全長cdna為3449核苷酸，其中5非翻譯區為876個核苷酸， 3非翻譯區為369個核苷酸並末端帶poly （ a ）尾巴，從起始密碼子atg至終止密碼子tga ，含有一個編碼768個氨基酸殘基的開放閱讀框架（ 2304bp ） ； abp640擴增片段的全長cdna為1012核苷酸，其中5非翻譯區為88個核苷酸， 3非翻譯區為144個核苷酸並末端帶poly （ a ）尾巴，從起始密碼子atg至終止密碼子taa ，含有一個編碼260個氨基酸殘基的開放閱讀框架（ 780bp ） 。The close genetic relationship of goose parvoviruse and aav allows the examination of the molecular biological properties of the nonstructural proteins of gpv. after the gpv infected the cell the viral life cycle was regulated by the nonstructural proteins encoded by the virus. according to the published of gpv b strain genome nucleotide sequences in genbank and a pair of specific primers were disigned with oligo4. 1
本研究根據genbank發表的gpvb株全基因序列，藉助oligo4 . 1軟體設計一對引物，採用pcr技術擴增gpvh1株非結構蛋白ns2基因，並與pmd18 - t載體連接后測序，結果表明：鵝細小病毒h1株ns2基因核苷酸全長1356bp ，編碼451個氨基酸殘基，與gpvb株的ns2基因相比，核苷酸數目相同，有17個堿基、 6個氨基酸的差異；同源性分析表明：二者核苷酸序列同源性為98 . 75 ，推導氨基酸序列同源性為98 . 67 。Thirteen putative epitopes showing characteristics of antigenic epitope were found from the analysis information. using pcr, the nucleotide acid fragments encoding these putative epitopes were amplified, then cloned into the expression vector miske. the positive recombinant phage displying the epitopes were found out by using pcr, sequencing and the determination of phage plaque titer
運用goldenkey分子生物學軟體對prrsvbj - 4結構蛋白的抗原表位及其二級結構進行了分析和比較，從中篩選13段顯示表位特徵的氨基酸殘基序列，用pcr技術擴增相應的核苷酸片段，將其插入到噬菌體表達載體m13ke ，結果預測的13個表位可在噬菌體表面得以展示。The cdna of subunit b was 1470 nucleotides long coding for 489 amino acids with a conservative atp binding site " 324 - sgsit - 328 " and a predicted molecular mass of 54. 29 kda. amino acid sequence alignment analysis suggested that the v - h + - atpase b subunit of s. salsa had high homology with other reported v - h + - atpase subunit b. the h + - atpase subunit h from s. salsa was a hydrophilic protein with 465 amino acids and a predicted molecular mass of 52. 8 kda, which was encoded by a cdna with 1398 nucleotides in orf. blast analysis indicated that the h + - atpase subunit h from s. salsa had a high amino acid sequence identity with those coming from plants, but had relatively low sequence identity with those coming from other species
鹽地堿蓬液泡膜h ~ + - atpaseb亞基是由1470個核苷酸編碼的長達489個氨基酸的多肽，分子量約54 . 29kda ，存在保守的atp結合序列「 324 - sgsit - 328 」 ，與其他物種來源的v - h ~ + - atpaseb亞基具有較高的氨基酸序列相似性； h亞基為親水多肽，開放閱讀框長達1398 - bp ，編碼465個氨基酸殘基，分子量約52 . 8kda ，與植物來源的液泡膜h ~ + - atpaseh亞基具有較高的氨基酸序列相似性，而與其他物種來源的v - h ~ + - atpaseh亞基同源性較低； c亞基開放閱讀框為495 - bp ，編碼164個氨基酸殘基，分子量約16 . 6kda ，為一跨膜多肽，存在四個可能的跨膜區。Its gene includes seven exons ( 3 - 9 ) and six introns ( 3 - 8 ). with respect to zfghrl, it is also like other ghr1s, which consists of 1578bp and encode a 528 amino acid protein. its gene only includes eight exons ( 1 - 5, 8 ) and seven introns ( l - 5 ). various methods used to find out the transmembrane and boxl domain of zfghrl but the result turns out to be fruitless due to unknown reason. fghr2 and zfghr2 : fghr2 includes eight exons and seven introns, which consists of 1710 bp and encodes a 569 amino acid protein ; zfghr2 includes eight exons ( 1 - 5, 8 ) and seven introns ( 1 - 5 ), which consists of 1758 bp and encodes a 484 amino acid protein
得到的fghr1含有7個外顯子（ 3 - 9 ）和6個內含子（ 3 - 8 ） ，由1755個bp的核苷酸組成並編碼一個584個氨基酸殘基的蛋白質：得到的zfghr1包含有1587個核苷酸並編碼一個528個氨基酸殘基的蛋白質，理學碩士學位論文:南方貼生長激素受體cdna的分子克隆和魚類存在兩種生長激素受體的證明其基因只有8個外顯子（ l一5 ， 8 ）和7個內含子（ 1一5 ） 。分享友人