根基菌素 的英文怎麼說
中文拼音 [gēnjījūnsù]
根基菌素
英文
radicalisin-
In this study, the stem segments of new shoot with axillary buds of well - growth tetraploid black locust trees were used as explants. the effects of different basic mediums, different hormone kinds and their concentrations ratios, different sucrose concentrations on calli induction, buds differentiation and rooting in the process of establishment of high frequency regeneration system of tetraploid black locust were studied. on the base of high frequency regeneration system, the effects of various factors on transformation efficiency of badh mediated by agrobacterium tumefaciens were discussed in the light of gus histochemical assays
本實驗首先以生長良好的四倍體刺槐優株上當年生新梢的帶腋芽莖段為外植體，研究了在四倍體刺槐高頻再生體系的建立過程中不同基本培養基、不同激素濃度及其配比、不同蔗糖濃度對愈傷組織的誘導、芽的分化及生根的影響；然後在得到高頻再生體系的基礎上，通過農桿菌介導法轉化甜菜堿醛脫氫酶（ badh ）基因，以gus染色組織分析為依據探討了影響轉化效率的各種因素，建立了高效、可重復的基因轉化體系，為四倍體刺槐目的基因的導入打下了基礎。In consideration of the colinearity between the activities of avermectin pks domains and structure of the polyketide product, the dh2 domain of avermectin pks, which corresponds to c - 22, 23 dehydration, appears to have partial dehydratase activity and this results in a mixture of c - 22, 23 double bonds ( " 1 " components ) and c23 hydroxy compounds ( " 2 " components )
目前國內外尚未見有關僅產阿維菌素b1菌株的報道。根據阿維菌素pks基因結構與其聚酮合成反應步驟之間的線性關系，推測b2組分的產生可能是由於阿維菌素pks模塊2中脫水酶（ dh ）的不完全活性所造成。Results bacterium that isolate take blue negative fungus of leather as the core, among them with copper green false form afterbirth fungus and uncle of pneumonia crayresearch as the main fact fungus, leather getring blue in the positive fungus in order to solidify enzyme negative staphylococcus and getting golden yellow staphylococcus comparatively common ; carbon that alkene antimicrobial it able to bears that medicine is minimum in rate, very beginning spore fungus kind, quinoline promise ketoneses plain, can according to medicine results quick as the clinical to use first - selected at glucosides amino sugar
結果分離出的細菌中以革蘭氏陰性菌為主，其中以銅綠假單胞菌和肺炎克雷伯菌為主，革蘭氏陽性菌中以凝固酶陰性葡萄球菌和金黃色葡萄球菌較為常見；碳青黴烯類抗菌藥的耐藥率最低，頭孢菌素類、喹諾酮類、氨基糖苷類可根據藥敏結果作為臨床使用首選藥。Ssmapkk transformations were screened on media with kanamycin ( 30mg / l ). nineteen individual kanamycin resistant plants were obtained. t2 plants were checked for integration of foreign gene by counting ratio of the number of tolerant plants to the number of non - tolerant plants on selection medium with kanamycin ( 30mg / l )
將ssvp和ssmapkk的全長cdna分別克隆入植物表達載體pcambia1300和prok中，導入根瘤農桿菌gv3101后，由花浸泡法進行擬南芥遺傳轉化，轉化ssvp鹽地堿蓬ssop和ssmapkk基因的克隆與功能鑒定的擬南芥在含潮黴素（ 25mg ）的ms培養基上篩選，獲得t ；代轉基因植株。But in s. mycarofaciens, there is no distinctive propionate kinase, so that the utilization rate of propionic acid is lower than that of acetic acid. for this reason, it is hoped that the higher producers would be obtained by protoplast interspecies fusion between s. mycarofaciens and s. erythreus, which would transfer propionate kinase of s. erythreus to s. mycarofaciens. therefore, the fusion cells could use propionic acid as precursor in synthesis of mdma _ ( 1 ), which would reduce the production costs
根據文獻報道，紅黴素鏈黴菌中的丙酸激酶對丙酸的利用率是對乙酸的利用率的13倍；而在生米卡鏈黴菌中，無特異的丙酸激酶，菌體對丙酸的利用低於對乙酸的利用，因此希望利用原生質體種間融合的方法將紅黴素鏈黴菌的丙酸激酶基因轉移到生米卡鏈黴菌中，從而使融合子能夠利用丙酸鹽作為合成mdma _ 1的前體，提高mdma _ 1的產量，降低生產成本。With high concentration of sds and elevated temprature （ 43 ） ， we succeeded in eliminating plasmids from ten strains of s. aureus which were isolated from the farms of beijing suburb. according to the sensitivity test before and after plasmid remoral ， we found that 72. 5 antibiotic resistance of s. aureus was mediated by plasmids ， and 27. 5 encoded by chromosome. the curing test indicated that the plasmids play an important role in coding the resistance to antibiotics
從北京郊區雞場分離到10株耐藥性金黃色葡萄球菌，對這些菌株進行質粒消除，根據質粒消除前後的耐藥性檢測，發現耐各種藥物的抗性基因大多數位於質粒上，少部分抗性基因位於染色體上，由質粒介導的耐藥基因佔72 . 5 % ，由染色體編碼的抗性基因佔27 . 5 % ，說明質粒在決定對抗生素的抵抗性中起主要作用。These results revealed that halocin c8 is cytocidal and its primary target might be located in the cell wall of the sensitive cells. two degenerate oligoxynucleotide primers ( c8 - 2, c8 - 4 ) were designed according to the n - terminal amino acid sequence of halocin c8
根據嗜鹽菌素cs的n端氨基酸序列設計了一對簡並引物（ cs一2 ， cs礴） ，利用這對引物從as7002的總dna中擴增出halcs的部分序列。In order to obtain a dna fragment containing aved gene, 5 ' end primer and 3 ' end primer, consisting of 18 and 20 nucleotides respectively, were designed and synthesized. a 2. 2 kb dna fragment containing aved gene was obtained by pcr when genomic dna of streptomyces avermitilis s - 2 was used as a template
為了得到aved基因片段，首先根據已知的阿維菌素生物合成基因簇的核苷酸序列，設計併合成了由18和20個核苷酸組成的5端和3端引物；以streptomycesavermitiliss - 2基因組dna為模板，經pcr擴增得到了2 . 2kb的含有aved基因的dna片段。The ced - 9 gene was transformed into leaves of tobacco by agrobacterium tumefaciens eha105. after twice selections of kanamycin cultures, 96 plants were regenerated from selective medium, and about 34 % of them turned out to be transgenic
然後以pbi121為對照組，通過根癌農桿菌eha105的介導，將ced - 9基因導入煙草葉片組織中，通過兩輪抗性篩選，得到抗卡那黴素（ km ）的再生植株。分享友人