根癌農根菌 的英文怎麼說
中文拼音 [gēnáinónggēnjūn]
根癌農根菌
英文
agrobacterium tumefaciens-
A new transgenic system of gerbera mediated by agrobacterium tumefaciens
小細胞團為受體的根癌農桿菌介導的非洲菊基因轉化體系The dna sequence had a complete orf ( open reading frame ), which coded a protein of 479 amino acid residues. the protein sequence of enolase which contained the conserved domain, was homologue to enolase of other organisms. it showed 83 % ideaity and 89 % similarity compared to the enolase in chlamydomonas reinhardtii
並將其中的一個gus基因用目的片段烯醇酶基因替換，構建了可以在植物中高效表達的載體pcambia2301g一enolase ，成功地將其轉入根癌農桿菌eha105中，為下一步進行轉基因植物的研究作準備。The materials as explant in transformation come from birch leaf, stem segment and leaf stalk, and the spider toxin gene was used as foreign gene for this transformation experiment. it showed that the best explant was the big leaf, on which the transformation frequency was 22 %. by gus detection, there were 43 percent of the plants with kanamycin resistance, and 100 percent of positive result, by pcr amplification, was gotten from random sampling
利用雙元載體的根癌農桿菌lba4404菌株（ agrobacteriumtumefaciens ） ，含質粒pyhy （目的基因及npt 、 gus基因） ，對白樺試管苗莖段，葉柄，葉片三種外植體進行侵染，結果表明：大葉片生長勢強，為轉基因的最優外植體，轉化率能夠達到22 。Agobacterium tumefaciens strain a311 carrying the plant tranfer vector pb1121, which contains the neomycin phosphotransferasell gene ( nptll ) and p - glucuronidase reporter gene ( gus ) both under the control of the camv 35s promoter, was used in the establishment of the genetic tranformation of white clover
選用苗齡4 5天的帶柄子葉作為外植體，先將外植體預培2天，再與根癌農桿菌a311共培養3 4天後，轉入附加有40mg l ~ （ - 1 ）卡那黴素和400mgAgrobacterium - mediated transformation of citrus embryoids and mature shoots
根癌農桿菌介導的柑桔遺傳轉化技術Difference of a grobacterium - mediated transformation in citrus cultivars
柑橘不同品種對根癌農桿菌介導遺傳轉化的反應差異Genetic transformation mediated by agrobactium tumefaciens in potato solanum tuberosum l
根癌農桿菌介導的馬鈴薯遺傳轉化體系的研究Optimization of a. tumefaciens - mediated transformation procedure for lotus corniculatus
根癌農桿菌介導的百脈根遺傳轉化體系的優化研究Effects of antibiotics on inhibition of agrobacterium tumefaciens and differentiation of tobacco leaf
抗生素對根癌農桿菌的抑菌效果及對煙草葉片分化的影響Agrobacterium a genus of soil bacteria, the species a. tumefaciens being the causative agent of crown gall, a type of tumor in plants
根癌農桿菌：土壤中存在的一種農桿菌，可以感染雙子葉植物的受傷組織誘發一種植物的腫瘤冠癭瘤。The aim of this research is to establish a transgenic system for flower plants and investigate the functions of mapkk in transgenic plants in the future
本研究構建了野生型、突變型mapkk基因植物表達載體，並用凍溶法將其導入了根癌農桿菌lba4404 。Tissue culture of soybean ( glycine max l ) and stem mustard ( brassica juncea mao ) in vitro and genetic transformation of soybean by agrobacterium tumefaciens were studied in this experiment
本論文研究了大豆的離體再生和根癌農桿菌介導的遺傳轉化及榨菜的離體培養。In this construct, hf2 dna should be expressed under the control of the camv 35s promoter. the construct was transformed to petunia hybrida of the light pink via agrobacterium tumefaciens eh a105 using the leaf disc method. in the end, three transgenetic plants were obtained by screening with the phosphinothricin resistance and pcr amplification
通過三親交配將重組質粒pc3301 - hf2導入根癌農桿菌eha105 ，抗性篩選、 pcr檢測及dna點雜交表明轉化后的農桿菌帶有完整目的基因片段，能夠用於轉化植物。It shares less than 25 % homology with the epsp synthases in class ii. it also shares 24. 5 % homology with the epsp synthase isolated by monsanto company and does not contain any conservative sequences and mutational sites protected by patents. all of the above shows great potential for future development
與class的epsp合成酶同源性低於25 ，其中與monsanto公司分離的根癌農桿菌（ agrobacteriumtumefaciens ） cp4來源的epsp合成酶同源性僅為24 . 5 ，且不含有專利保護的保守序列和突變位點，顯示了良好的開發應用潛力。The first two reports of soybean transformation used two very different methods to transform soybean : hinchee et al ( 1988 ) used agrobacterium - mediated transformation of cotyledonary nodes while mccabe et al ( 1988 ) used particle bombardment of shoot meristems. soybean transformation reports following these initial papers have been limited and the transformation efficiency of soybean has remained low, the reason is that cells that are regeneration - competent must also be accessible and transformation - competent
目前應用於大豆遺傳轉化的方法主要有根癌農桿菌介導法，基因槍法，超聲波輔助農桿菌法，真空抽濾法，花山東師范大學碩士學位論文粉管導人法等，但其中獲得廣泛應用的只有根癌農桿菌介導法和基因槍法，但是這兩種方法在應用中也有一定的局限性。In this paper, we transferred ced - 9 gene, an anti - apoptosis gene, into tobacco and rice by agrobacterium tumefaciens eha105. the results are showed as follows : 1
Ced - 9是發育pcd的負調控基因，本課題將ced - 9基因通過根癌農桿菌（ agrobacteriumtumefaciens ） eha105介導轉化煙草和水稻，獲得以下結果： 13. potato stems and agrobacterium fumefaciens containing recombinant vector were co - cultured at 28cfor 48 hours and transplanted onto callus - inducing medium at 24c for 7 days. and then, the explants were transplanted onto differentiation medium and cultured at 24c for 21 days. resistant buds rooted and grew into plants in medium with kanamycin for 20 days, and 83 plants were obtained
3將含外源基因的根癌農桿菌與馬鈴薯莖段共培養后在愈傷誘導培養基上培養7天，轉接到分化培養基上分化出抗性芽，抗性芽在生根培養基上生根長成完整植株，共獲83株。The recombinant vector pbi121 containing si gene of ibv was confirmed by pcr and double digestion of restriction endonuclease. agrobacterium fumefaciens eha105 with the recombinant vector pbi121 was obtained by tri - parental mating method
用三親交配法將重組克隆導入根癌農桿菌，獲得了含pbi121重組質粒的eha105農桿菌菌株。Svde gene was also amplified from spinach by rt - pcr. the plant expression vector pcb - antisvde was constructed, in which svde gene was controlled by camv35s promoter and nos terminator. antisense transgenic tobacco plants v. ere obtained from leave explants via agrobacterium tumefaciens - medialed transformation
從菠菜中克隆了svde基因，並構建了該基因的反義抑制植物表達載體pcb - antisvde ，用根癌農桿菌介導法轉化煙草，獲得了大量的轉基因植株。The ced - 9 gene was transformed into leaves of tobacco by agrobacterium tumefaciens eha105. after twice selections of kanamycin cultures, 96 plants were regenerated from selective medium, and about 34 % of them turned out to be transgenic
然後以pbi121為對照組，通過根癌農桿菌eha105的介導，將ced - 9基因導入煙草葉片組織中，通過兩輪抗性篩選，得到抗卡那黴素（ km ）的再生植株。分享友人