桿狀物 的英文怎麼說
中文拼音 [gǎnzhuàngwù]
桿狀物
英文
shaft-
The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中,通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中,然後採用葉盤法,在該農桿菌的介導下將pap基因導入普通煙草中,經過卡那黴素抗性篩選,最後獲得了轉pap基因的工程煙草植株,摩擦接種煙草花葉病毒( tmv ) ,與非轉基因煙草相比,能夠推遲癥狀表現達2月之久,說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。This twin screw extruder has the feature of one - side circumgyrate , it ' s an ideal equipment for the small - scale factory who produce powder
該擠出機是同向高速旋轉雙螺桿結構,是用於多種組份物料經一定的溫度在熱狀態下達到最佳的理想效果,適應小型企業生產粉末的理想設備。Microbiology of food products. preserves. detection of clostridium thermophilus
食品微生物學.罐頭.嗜熱梭狀芽孢桿菌檢查The brewing characteristics of shaoxing wine were presented in the following eleven aspects : the uniqueness of adjunct materials and the variety of different wine, the treatment of soaking rice at low temperature for a long time, the variety of fermentation state, the characteristics of rice wine broth, the variety and complexity of microorganisms, the uniqueness of inoculation and strain preservation, the process of mixed fermentation of yeast and lactic acid bacteria ( e. g. the concurrence of saccharification, yeast fermentation and lactic fermentation ), the over - mixing of different liquors, the high sterilization temperature, and the storage of produced wine
摘要該文從11個方面論述了紹興黃酒的釀造特點,即配料的特殊性和酒種的多樣性;低溫長時間浸米;發酵狀態的多樣性;黃酒醪的發酵特點;微生物的多樣性,復雜性;接種方式獨特;菌種保存方法獨特;酵母與乳酸桿菌協同作用的混合發酵并行的過程(即邊糖化、邊酵母發酵、邊乳酸發酵同時進行的三邊發酵) ;酒液勾兌;較高的滅菌溫度;成品酒的貯存。In the thesis, we tried to transfer the novel non - lytic system to suspension culture in spinner flask
另外一方面,有文獻利用桿狀病毒作為載具,將外來基因送入哺乳動物細胞表現,且不會對哺乳動物細胞的生長造成影響。The reasults are summed up as following : 1 the study on chromosomes and mitoses of bmn cells the cell line, bmn, is a silkworm cell line widely used in silkworm molecular genetics, cell engineering, gene engineering and baculovirus expression system but whose genetics and cytobiology studies are nearly untouched. the chromosomes and mitoses of the bmn cells are researched by the air - drying method and culturing cells on cover glasses
同時,還通過原代培養實驗對新的家蠶胚胎細胞系的建立進行了探索和嘗試,並對家蠶胚胎原代培養過程中出現的細胞和組織類型進行了觀察、探討與研究。 1bmn細胞有絲分裂及染色體研究bmn細胞是家蠶分子遺傳學,細胞工程、基因工程和桿狀病毒表達系統中廣泛應用的家蠶細胞,但其遺傳學和細胞生物學背景知之甚少。Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )
利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠,然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞( cef )做免疫熒光試驗( fa ) ,進行其分泌抗meq單克隆抗體( mcab )能力的篩選。The protein product of meq gene was highly expressed in the nuclei of recombinant baculovirus infected sf9 cells when using an anti - meq monoclonal antibody ( mcab ) 23b46 to run the immunofluorescence assay ( fa ) ; the expression quantity and if staining patterns differed with different times post - infection ( pi ). the results of western blotting and immunoprecipitation test showed there were two specific bands around 60 kd. the results of the study demonstrated that the baculovirus / insect cell system is effective to be used to express nuclear protein of virus
結果發現:本表達系統產生的meq蛋白可被重組痘病毒表達的meq制備的單抗23b46所識別;在感染細胞中, meq蛋白僅局限於細胞核內,而且隨著感染后( pi )時間的增加,具有從核質向核仁和核膜轉移的趨向; w已stemblot和免疫沉澱試驗均證實重組桿狀病毒感染細胞裂解物中出現有兩條大小約為60kd的特異帶。Baculoviruses are arthropod specific viruses that have long been used as biological agents to control the insects attacking crop plants
摘要桿狀病毒是鱗翅目昆蟲的重要病原微生物,在重要農業害蟲的生物防治中具有很大的應用前景。Maybe thousands of mine shafts
蜂窩結構的桿狀物A bar used with a double harness to connect the collar of each horse to the pole of a wagon or coach
軛狀物用雙馬具把每個馬軛連到四輪運貨馬車或公共馬車的轅桿上的木條The gradual development of these building blocks, following the invention of the roller or wheel and the arm or lever in ancient times, brought about the industrial revolution, starting with the assembly of james watt ' s engine for harnessing the force of steam and proceeding into the advanced mechanization of present automatic control
隨著遠古軋輥、輪子及臂狀物或杠桿的發明,這些構建塊逐漸發展起來,並帶來了工業革命並以詹姆斯?瓦特的以蒸汽為動力為開始,現在已進入到高級機械自動化的階段。The recombinant meq - baculovirus was obtained by co - transfecting the insect sf9 cells with pblubac4 - meq and linearised bac - n - blue dna. the recombinant baculovirus was selected by plaque assay and confirmed by pcr technique and sequencing of the inserted gene
應用重組痘病毒表達的meq制備的單抗23b46對重組桿狀病毒感染的sfg細胞及其裂解物分別進行間接免疫熒光試驗、 westemblot和免疫沉澱試驗的檢測。Several baculoviruses such as acmnpv, ldmnpv and busunpv contain conotoxinlike genes, but the biological function of these genes is unknown
桿狀病毒類蝸牛毒素是一類小分子肽,在acmnpv 、 ldmnpv和busunpv等一些桿狀病毒基因組中存在,但其確切的生物學功能尚不清楚。Escherichia coli a rod - shaped gramnegative bacterium, some strains of which are normally present in animal intestines, soil, or water
大腸桿菌:是一種桿狀的格蘭氏陰性菌,通常存在於動物腸內、水中或土壤中。This dissertaion, on the basis of the other studies, on the one hand, researched and analysed the chromosomes and mitoses of the cell line - bmn, which is widely used in the silkworm baculovirus expression system as an engineering cell. on the other hand, the dissertation attempted to explore establishment of the new silkworm embryoni cell line by primary culture
同時,家蠶體外細胞培養研究的進行,不僅可以為家蠶細胞生物學的基礎理論研究提供良好的研究系統,而且在家蠶資源的開發與利用方面也具有重大的意義。本研究在借鑒前人研究的基礎上,一方面對現在廣泛應用於家蠶桿狀病毒表達系統的工程細胞? bmn細胞的染色體和有絲分裂進行了研究與分析。For mass production of egf, bombyx mori baculovirus expression vector system ( bevs ) was adopted in this experiment to express recombinant egf
為大量獲得egf ,本研究利用家蠶桿狀病毒表達系統,以家蠶作為生物反應器,在家蠶細胞和幼蟲中表達人表皮生長因子。Baculovirus is a kind of double - stranded circle large dna virus, which has been developed as an environment sound pesticide and a powerful vector of foreign protein expression as well as a potential vector of gene therapy
桿狀病毒是一類雙鏈環狀dna病毒,作為生物防治殺蟲劑及高效外源蛋白表達載體得到廣泛研究。近來有許多學者致力於桿狀病毒作為基因治療載體的研究。Although compared with the other insect cell expression systems, the expression system which uses silkworm, bombyx mori, as bioreactor is very meaningful to study for so many merits of it
雖然以家蠶為生物反應器的家蠶桿狀病毒表達系統較之其他的昆蟲表達系統來說具許多優點,具有重大的研究意義。To overcome these limitations, we produced recombinant an - giostatin in a baculovirus expression system. here we report the expression and analysis of recombinant human angiostatin produced by insect cells infected with recombinant angiostatin - baculovirus. methods generation of recombinant baculovires by co - transfection
為克服這些困難,獲得具有高活性的angiostatin表達,為進一步研究及應用奠定物質基礎,我們通過桿狀病毒表達系統表達重組的angiostatin ,本研究就是在昆蟲細胞中表達和分析angiostatin ,並研究其生物活性。分享友人