桿胞菌素 的英文怎麼說
中文拼音 [gǎnbāojūnsù]
桿胞菌素
英文
bacillosporin-
The total rna was isolated from pokeweed ( phytolacca americana ) leaves using the method of guanidine isothiocyante and used as template to amplify the total length and deleted mutant pokeweed antiviral protein ( pap ) gene by rt - pcr and then the pap gene was cloned into pgem - t vector. the sequencing results showed that pap gene had 99. 9 % identity comparing with the pap gene nucleotide sequence reported by lin et al ( 1991 ). the iptg - inducible expression vector containing the pap gene was constructed and transferred into e. coli bl21 ( de3 ) - plyss
將缺失型pap基因克隆到植物表達載體pbi121中，通過液氮冷凍法將重組質粒轉入農桿菌lba4404細胞中，然後採用葉盤法，在該農桿菌的介導下將pap基因導入普通煙草中，經過卡那黴素抗性篩選，最後獲得了轉pap基因的工程煙草植株，摩擦接種煙草花葉病毒（ tmv ） ，與非轉基因煙草相比，能夠推遲癥狀表現達2月之久，說明pap基因能夠在其它植物體內產生有活性的高抗病毒的蛋白質。It is an important that bacteria contaminated vaccine in the biologicals production. we collected 703 samples of cell culture, virus cultivation and harvest which were contaminated by bacteria during poliovaccine production within two years. we checked these samples by bacteriological method and antibiotics sensitivity tests were done. it shows that 1 ) the main contaminated bacteria come from staphylococci, bacilli and streptococci of environment in the poliovaccine production. 2 ） it is effect that antibiotics to contaminated bacteria are doxycycline, albiotic, prescription 2, cefotaxime na salt, gentamycin, neomycin, aureomycin and erythromycin
在疫苗生產實踐中，細菌污染是影響疫苗質量和產量的關鍵性因素，筆者通過了兩年左右的時間，選取正常生產中零星細菌污染的細胞培養瓶、病毒培養瓶及收毒污染樣品等共703份，進行細菌學檢查，並對造成污染的主要細菌種類進行了各種抗菌藥物的耐藥性實驗，結果表明：我所脊灰疫苗生產中主要的污染威脅來自環境中的葡萄球菌，潛在威脅是桿菌和鏈球菌；強力黴素、林可黴素、配方2 、噻孢黴素鈉鹽、慶大黴素、新黴素、金黴素和紅黴素等抗生素對目前引起污染優勢細菌-葡萄球菌有明顯的抑菌效果，可作為疫苗生產后備抗菌手段參考The bacilliform cell penetrate into interior of the fibre to degrade the cellulose strongly and produced a mass of sticky polysaccharides. after cultured 48 hours, the bacilliform cell ' s surface of sporocytophaga have a great change. at this stage the bacilliform produce a lot of sticky polysaccharides. these sticky polysaccharides associated with the sites where the filter paper was decomposed intensively and form thorns on the surface of the bacillium. at the same time, the filter - paper weight loss is the greatest and decomposing rate is the fastest, so we think that the sticky polysaccharides are produced during the cellulose degradation
培養48小時，桿狀細胞的表面結構發生很大的變化，此時的菌體表面已產生大量的粘性多糖，這些粘性多糖因菌體在纖維素表面滑動而在菌體表面形成突起，即在纖維素被旺盛降解部位的菌體表面產生了大量突起；而產生突起的菌體深入到纖維素分子內部，纖維素表面可以清晰地看到由於菌體嵌入纖維素分子內部而留下的凹陷。All of our products is exported likelactobacillus acidophilus, bidifobacterium longum, lactobacillus paracasei, lactobacillus rhamnosus, tagatose, lnulin, nisin, natamycin, - polylysine, nucleotides mix, adenosine monophosphate, cytidine monophosphate, guanosine monophosphate disodium, uridine monophosphate disodium, calcium inosinate, calcium guanylate, calcium 5 - ribonucleotide, damp, dcmp, dgmp, tmp, deoxyadenosine, 2, 6 - diaminopurine nucleoside, 2, 6 - diaminopurine deoxynucleoside, adenine arabinoside, adenosine triphosphate, uridine triphosphate, guanosine triphosphate, cytidine triphosphate, cytidine diphosphate choline （ citicoline ） to all over the world, if you are interested in our products, please do visit our website, hope there will fullfill your requirement
主營產品有益生菌（嗜酸乳桿菌、長雙歧桿菌、乾酪乳桿菌、鼠李糖乳桿菌） 、塔格糖、菊粉（菊糖） 、乳酸鏈球菌素、納他黴素、 -聚賴氨酸、核苷酸，腺苷酸，胞苷酸，鳥苷酸二鈉，尿苷酸二鈉，肌苷酸，鳥苷酸鈣，核糖核苷酸鈣，脫氧腺苷酸，脫氧胞苷酸，胸苷酸二鈉，脫氧鳥苷酸二鈉，脫氧腺苷， 2 -氨基脫氧腺苷， 2 -氨基腺苷，阿糖腺苷，胞二磷膽堿，腺苷三磷酸，三磷酸尿苷，三磷酸胞苷，三磷酸鳥苷等The hemagglutinating activity of the fluids was not affected by treatment with 50 mm edta and egta, while it was increased by treatment with 3 - me
經注射雞大腸桿菌后，文昌魚體液中的凝集素對人b 、 o型紅血細胞，兔、草魚和蟾蜍紅血細胞的凝集活性明顯增強。Thus, it is postulated that in the presence of mild neutropenia, whose function is inhibited by effect of insulin excess, the bacillus was able to find a port of entry, probably via micro - abrasions of the bowel mucosal lining
因此，推測可能存在中性粒細胞減少，功能被過多的胰島素抑制，芽孢桿菌可以乘虛而入，很有可能通過腸內壁粘膜微破損進入循環。Acellular as well as inactivated polioviruses types i, ii iii. the old dtwp vaccine is also a combination vaccine with similar components for diphtheria and tetanus but contains the " whole - cell " component for pertussis and does not contain the poliovirus component
新疫苗的白喉及破傷風類毒素成份與以往所用的三合一白喉破傷風百日咳混合疫苗dtwp相若，而以往所用疫苗的百日咳桿菌為全細胞型及不含小兒麻痹病毒。As starter cultures, twelve strains of bacillus were screened for the manufacture of long - ripened douchiba from 40 strains isolated from naturally fermented douchiba samples at consecutive stages from qianxi and dafang of guizhou province, through comprehensive evaluation including extracellular proteolytic activity and lipidolytic activity, salt tolerance, ultraviolet light resistance, and antibiotic sensitivity
摘要從貴州黔西和大方兩地傳統陳窖豆豉粑不同工藝階段分離到的40株菌株，經胞外蛋白質和脂肪水解酶活力、耐鹽性能、抗紫外光照射和抗生素敏感性等綜合性能評價，篩選到12株芽胞桿菌可作為陳窖豆豉粑純菌發酵的選擇菌株。Kurstaki strain hd73, were inserted into two copy sets of res sites. the res sites have same direction. when - the recombinant plasmid was introduced into crystal negative b. thuringiensis host bmb171, antibiotic resistance genes and other non - 5, thuringiensis dna can be selectively eliminated after the selection by antibiotic resistance marker
將crylac10基因或壯觀黴素基因和蘇雲金芽胞桿菌的質粒復制起始區oril030連接在一起，置於兩個同向的解離區之間，再將基因操作中所必需的大腸桿菌質粒復制起始區和抗生素標記基因等與之相連構成解離載體。The minimum inhibitory concentrations ( mic ) of imipenem, panipenem and meropenem for 225 clinical isolates was determined by agar dilution method, in comparison to 13 other antimicrobial agents
結果，三種碳青黴烯類抗生素對腸桿菌科細菌具高度抗菌活性，對銅綠假單胞菌、不動桿菌屬、糞腸球菌等亦具良好抗菌作用。The interesting gene fragment with ecori and noti were amplified by overlapping pcr, which inserted into vector plasmid ppic9k after degisted by ecori and noti, and the recombinant plasmid was transformed into competent dh5cc. positive clones were screened by pcr from the lb plate with amp. digesting analysis resulte shows that the interesting gene were inserted into the vector ppic9k with correct direction
目的基因經雙酶切后連接載體ppic9k ，然後導入大腸桿菌dh5中，在含氨卞青霉素（ amp ）的lb板上用pcr反應篩選出陽性菌落，雙酶切結果表明目的基因已插入載體中，且方向正確，測序結果進一步證明人巨細胞病毒重組基因表達質粒成功地克隆了目的基因片段。We obtained our transgenic material, the rice suspension cells, by inducing embryonic rice callus. then we constructed the expression vector pca - ced9, and transferred ced - 9 gene into the rice callus and embryogenic suspension cells. the work of using hygromycin selective medium to obtain regenerated plants is still going
構建了用於水稻中表達的載體pca - ced9 ，通過農桿菌eha105轉化導入水稻愈傷組織和水稻懸浮細胞，經潮黴素（ hym ）篩選，以期望獲得抗性植株，目前該工作仍在進行中。Because expressed in escherichia coli, ricin a chain is not degraded easily for its non - glycosylation. our experiments lay a foundation for the researches that rta conjugates a monoclonal antibody to synthesize immunotoxins and the immunotoxins are used for cancer - targeted therapy
蓖麻毒素a鏈在大腸桿菌中表達，因無糖基化修飾，克服了進入體內后被迅速降解的缺點，為進一步與單鏈抗體基因嵌合表達形成單鏈免疫毒素，對腫瘤細胞進行導向治療研究奠定基礎。The present investigation is favorable for screening the new generation of anti - tumor peptides. [ objective ] ( 1 ) to clone and express fasl - ecd in e. coli
[目的] （ 1 ）分析人黑色素瘤細胞（ a375 ）中fasl基因的表達，克隆人fasl胞外區的編碼基因，並在大腸桿菌bl21中進行表達。In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells
為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系，本實驗研究分為三個部分： （ 1 ） axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建； （ 2 ）探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理，並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響； （ 3 ） axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下： 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ，並將其構建入真核表達載體中，編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。Risk factors for entilator - associated pneumonia by pseudomonas aeruginosa in presence of recent antibiotic exposure
近期抗生素暴露患者由綠膿桿菌和假單胞菌性產生的與機械通氣相關肺炎的危險因素。Risk factors for ventilator - associated pneumonia by pseudomonas aeruginosa in presence of recent antibiotic exposure
近期抗生素暴露患者由綠膿桿菌和假單胞菌性產生的與機械通氣相關肺炎的危險因素。All these results suggested that the cause of 4d1 culture inhibiting the pathogenicity of scg1 is the decreased concentration of ahl molecule in scg1 cells, when presenting aii protein in 4d1 cells, and then not inducing the expression of virulence genes of scg 1 at low concentration of ahl molecules
由此推測，蘇雲金芽胞桿菌影響病原菌致病性的原因在於降低了病原菌細胞內ahl類信號分子的濃度，使其達不到激活毒素基因表達的水平。These anthraquinones are extremely cytotoxic ( they fight against ) towards a broad spectrum of bacteria, fungi, and viruses that include pneumonia, e coli, blood infections, diarrhea, skin infections and ( tuberculosis
蒽醌是一種極為有效的廣譜類抗細胞毒素物質，可抗擊多種細菌、真菌、病毒，包括導致肺炎的細菌和病毒、大腸埃希氏桿菌，以及導致血液感染、腹瀉、皮膚感染和肺結核的微生物。Chemical synthesis of human interleukin - 18 gene and its high - level expression in e. coli
人白細胞介素18基因化學合成及其在大腸桿菌中高表達分享友人