桿菌培養 的英文怎麼說

中文拼音 [gǎnjūnpéiyǎng]
桿菌培養 英文
bacilli culture
  • 桿 : 桿名詞（桿子） pole; staff
  • 菌 : 菌名詞1. （蕈） mushroom2. （姓氏） a surname
  • 培 : 動詞1. （在根基部分堆上土） bank up with earth; earth up 2. （有目的地使成長、壯大） cultivate; foster; train
  • 養 : Ⅰ動詞1 （供養） support; provide for 2 （飼養; 培植） raise; keep; grow 3 （生育） give birth to ...
  • 桿菌 : [微生物學] bacillus
  1. It is an important that bacteria contaminated vaccine in the biologicals production. we collected 703 samples of cell culture, virus cultivation and harvest which were contaminated by bacteria during poliovaccine production within two years. we checked these samples by bacteriological method and antibiotics sensitivity tests were done. it shows that 1 ) the main contaminated bacteria come from staphylococci, bacilli and streptococci of environment in the poliovaccine production. 2 ） it is effect that antibiotics to contaminated bacteria are doxycycline, albiotic, prescription 2, cefotaxime na salt, gentamycin, neomycin, aureomycin and erythromycin

    在疫苗生產實踐中，細菌污染是影響疫苗質量和產量的關鍵性因素，筆者通過了兩年左右的時間，選取正常生產中零星細菌污染的細胞培養瓶、病毒培養瓶及收毒污染樣品等共703份，進行細菌學檢查，並對造成污染的主要細菌種類進行了各種抗菌藥物的耐藥性實驗，結果表明：我所脊灰疫苗生產中主要的污染威脅來自環境中的葡萄球菌，潛在威脅是桿菌和鏈球菌；強力黴素、林可黴素、配方2 、噻孢黴素鈉鹽、慶大黴素、新黴素、金黴素和紅黴素等抗生素對目前引起污染優勢細菌-葡萄球菌有明顯的抑菌效果，可作為疫苗生產后備抗菌手段參考
  2. In this study, the stem segments of new shoot with axillary buds of well - growth tetraploid black locust trees were used as explants. the effects of different basic mediums, different hormone kinds and their concentrations ratios, different sucrose concentrations on calli induction, buds differentiation and rooting in the process of establishment of high frequency regeneration system of tetraploid black locust were studied. on the base of high frequency regeneration system, the effects of various factors on transformation efficiency of badh mediated by agrobacterium tumefaciens were discussed in the light of gus histochemical assays

    本實驗首先以生長良好的四倍體刺槐優株上當年生新梢的帶腋芽莖段為外植體，研究了在四倍體刺槐高頻再生體系的建立過程中不同基本培養基、不同激素濃度及其配比、不同蔗糖濃度對愈傷組織的誘導、芽的分化及生根的影響；然後在得到高頻再生體系的基礎上，通過農桿菌介導法轉化甜菜堿醛脫氫酶（ badh ）基因，以gus染色組織分析為依據探討了影響轉化效率的各種因素，建立了高效、可重復的基因轉化體系，為四倍體刺槐目的基因的導入打下了基礎。
  3. Optimization of one isolate bacillus subtilis

    一株枯草芽孢桿菌發酵培養基的優化
  4. The bacilliform cell penetrate into interior of the fibre to degrade the cellulose strongly and produced a mass of sticky polysaccharides. after cultured 48 hours, the bacilliform cell ' s surface of sporocytophaga have a great change. at this stage the bacilliform produce a lot of sticky polysaccharides. these sticky polysaccharides associated with the sites where the filter paper was decomposed intensively and form thorns on the surface of the bacillium. at the same time, the filter - paper weight loss is the greatest and decomposing rate is the fastest, so we think that the sticky polysaccharides are produced during the cellulose degradation

    培養48小時，桿狀細胞的表面結構發生很大的變化，此時的菌體表面已產生大量的粘性多糖，這些粘性多糖因菌體在纖維素表面滑動而在菌體表面形成突起，即在纖維素被旺盛降解部位的菌體表面產生了大量突起；而產生突起的菌體深入到纖維素分子內部，纖維素表面可以清晰地看到由於菌體嵌入纖維素分子內部而留下的凹陷。
  5. Obtaining transgenic male sterile tobacco in order to prove that hsp70 antisense cdna can lead to male sterility, with plasmid 3301 + 650, 3301 + 651 we transformed 207 aspetic tobacco leaves by genegun bombarding and agrobacterium mediation ( 109 by genegun bombarding, 98 by agrobacterium ). by cultivating them in blotting media containing basta 0. 4 mg / 1, we get 181 resistant leaves ( 98 by genegun bombarding, 88 by agrobacterium mediating )

    獲得轉基因雄性不育煙草為了證實hsp70反義cdna能創造雄性不育，我們將3301 + 650和3301 + 651質粒用基因槍和農桿菌介導法轉化煙草無菌發芽的葉片，共207片（基因槍109片，農桿菌98片） 。在含basta0 . 4mg l的篩選培養基上進行篩選，得到抗性葉片181片（基因槍93片，農桿菌88片） 。
  6. The results showed that : adding tryptone, soy peptone. beef extract, com extract and cys - hcl to jaj could obviously promote the growth of blm and bbm ; by the orthogonal experiment of three elements and three levels, a satisfying jaj compound medium was acquired which included corn extract ( 0. 3 % ), soy peptone ( 0. 05 % ) and cys - hcl ( 0. 025 % ). nextly, after establishing a selective bifidobacterium medium, the effects of jaj on the growth of bifidobacteria in vivo were studied, using healthy mouse of kunming species as experimental animal

    研究了以菊芋汁為主要原料的雙歧桿菌培養基，大量試驗結果表明，在菊芋汁中添加胰蛋白腖、牛肉膏、大豆蛋白腖、玉米漿和半胱氨酸鹽酸鹽等成分，對雙歧桿菌有明顯的促進生長作用；利用大豆蛋白腖、玉米漿和半胱氨酸鹽酸鹽設計了三因素三水平的正交試驗，確定了菊芋汁復合培養基的優化配方：菊芋汁+ 0 . 3玉米漿+ 0 . 05大豆蛋白腖+ 0 . 025半胱氨酸鹽酸鹽。
  7. Seed culture conditions of bacillus licheniformis ts - 01 were firstly investigated. the results showed that the optimal growth temperature was 40 c ; the suitable seed culture medium was beef extract soya peptone medium ; the optimal initial ph was 7. 5 ; the optimal seed culture time was 13h ~ 14h

    首先對地衣芽孢桿菌ts - 01的種子培養條件進行了研究，得到的結果為：最適生長溫度為40 ；較適合的種子培養基為牛肉膏大豆蛋白腖培養基；最適初始ph值為7 . 5 ；種齡為13h 14h 。
  8. In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli

    根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。
  9. Blood culture subsequently returned positive for clostridium septicum

    隨后的血培養敗血梭狀芽孢桿菌陽性。
  10. The cultural conditions such as temperature, fermentation period and the compound of medium are studied. the result of test show that suitable factors for both bacterium to grow and active substance to produce are 28, 200rpm and 72 hours. the bacterium is gotten through centrifuge with 8000rpm for 20min. then the bacteria is diluted and colophony named s - 8 is put into and used to absorbed active substance for 4 hours

    對該菌株發酵條件的研究表明：該菌株用馬鈴薯葡萄糖液體培養基發酵培養， 28 ， 200rpm搖瓶中振蕩培養72h可獲得高活性的發酵產物，用蘇雲金芽孢桿菌hd - 1做指示菌，將發酵液稀釋40倍生測仍可形成明顯的抑菌圈。
  11. The culture of the tiny piece of expectorated sputum yielded an organism known as mycobacterium avium intracellulare

    對這點樣品培養出來一種細胞內分枝桿菌。
  12. The results indicated that : jaj could selectively stimulate the reprduction of bifidobacteria in vivo and inhibit the growth of e. coli which is a main parasitic basterium in human intestinal tract ; moreover, jaj could apprarently improve intestinal tract function. in tested group, the mice excreted smoothly and the faecal particles of mice were big and wet, but in control group, the faecal particles of mice were small and dry. lt was suggested that inulin may be the important effective component in jaj which promoted the reprduction of bifidobacteria in vivo. at last, the effects of ja on the bile salt resis tance of bifidobacteria were studied. the test proved that : deoxycholic acid na - salt ( dca - na ) had intensely toxical action on blm and bbm ; adding glucose and fructose in media could decrease the lexical action on bbm. but inulin and jap had not apparent effect

    在通過單菌株檢驗和混菌檢驗確立了一種選擇性雙歧桿菌培養基之後，進一步以健康昆明系小鼠為實驗動物，研究了菊芋在動物腸道內對雙歧桿菌的影響，動物實驗結果表明，菊芋汁在體內對雙歧桿菌有選擇性促進生長作用，而腸道中主要條件致病菌?大腸桿菌的生長受到抑制；菊芋中的菊糖成分可能對菊芋在體內選擇性地促進雙歧桿菌生長起了主要作用；此外，菊芋還具有明顯的整腸作用，同對照組相比，飼喂菊芋汁的小鼠排便順利，糞便顆粒大且濕潤。
  13. In order to provide theoritical foundation for the utilization of jerusalem artichoke ( ja ) resource, culturation of bifidobacteria and development of bifidobacteria products. this paper rather systernly studied the effects of ja on the growth of bifidobacteria, using jerusalem artichoke juice ( jaj ) and jerusalem artichoke powder ( jap ) as experimental material. in the first place, the effects of ja on the growth of bifidobacteria in vitro were studied. the results indicated that : jaj could stimulated the growth of b. longum ( blm ) and b. bifidwn ( bbm ) ; the more jaj content was added, the more promoting action was apparent ; the promoting action also varied from the different species. adding jap ( 3 % ) to skim milk could decrease the time of milk - solidfying of blm and bbm, and could increase the acid production in skim milk of the strains tested. secondly, bifidobacterium medium was developed using jaj as main material

    本文以菊芋汁和菊芋粉為主要材料，較系統地研究了菊芋對雙歧桿菌生長的影響，以期為進一步開發利用菊芋資源、雙歧桿菌的培養及其製品開發提供理論依據。菊芋在體外對雙歧桿菌生長的影響試驗表明，菊芋汁在體外對長雙歧桿菌（ blm ）和兩歧雙歧桿菌（ bbm ）的生長具有促進作用，其效果隨著菊芋汁添加量的增加而增加，並且對不同菌種的促生長效果存在差異；在脫脂乳中添加3的菊芋粉可以縮短blm和bbm的凝乳時間，其原因可能為促進了試驗雙歧桿菌在脫脂乳中的產酸。
  14. The suitable concentration of the selective agent kanamycin is 50mg. l - 1. the duration of infection and co - culture with agrobacterium influences soybean transformation evidently

    農桿菌侵染時間和共培養時間對大豆的轉化有明顯的影響，侵染時間以15min為宜，共培養時間以3d為宜。
  15. This experiment passing to grope for the carbon source constitutes of the culture medium and using t. reesei rut c - 30 induced the expression of # - mannanase ( # - 1, 4 - mannan mannohydrolase ec 3. 2. 1. 78 ). in this experiment i put the constant carbon source ( lactose and locust bean gum ) in the foundation culture medium ( mandels nourishment liquid ) of t. reesei rut c - 30, then proceeded the variable carbon source ( dragon spruce fiber, com rush pith fiber, wheat straw fiber, wheat straw xylan, corn rush pith xylan, dragon spruce mannan ) to single factor, double factor, three factor, four factor and five factor orthogonal experiment. 1 determined the activity of p - mannanase using locost bean gum as substract by the 3, 5 - dinitosalicylic acid method, and observed the growing situation of the gernic at the end i selected the directions for the inducement expression of the # ? mannanase from trichoderma reesei rut - c30 that contained the dragon spruce fiber, wheat straw xylan, dragon spruce mannan

    在里氏木霉rutc - 30的基礎培養基（ mandels營養液）中加入固定碳源乳糖和槐豆膠，然後將可變碳源（雲杉纖維、玉米芯纖維、麥桿纖維、麥桿木聚糖、玉米芯木聚糖、雲杉甘露聚糖）進行單因子、雙因子、三因子、四因子、五因子的里氏木霉rutc - 30正交培養實驗，並以槐豆膠為底物用3 ， 5二硝基水楊酸法測定培養液中?甘露聚糖酶的活力。從而確定了酶活最高且菌體生長良好的含雲杉纖維、麥桿木聚糖和雲杉甘露聚糖的誘導培養基為最佳培養基，用該培養基培養的里氏木霉（ t . reesei ） rutc - 30使其轉錄的-甘露聚糖酶（ - 1 ， 4 - mannanmannohydrolaseec3 . 2 . 1 . 78 ） mrna量能夠滿足rt - pcr的要求。
  16. 5. after induced with iptg, jm109 with pgex - 4t - l / 6 - 4 was radiated by uv for 30 seconds, cultivate these e. coli with light or without light. the survival rate proved the gene of d. salina ( 6 - 4 ) photolyase has photoreactivation

    5 .工ptg誘導過的轉化菌，經過30秒的紫外光照射后，分別進行光培養或暗培養，計算存活率，從而證明了及sa了z 』 na的（ 6一4 ）光裂合酶在大腸桿菌中可進行光修復活性作用。
  17. Optimization of reproducible wort medium for bifidobacteria

    雙歧桿菌麥芽復合汁增菌培養基優選的研究
  18. Agobacterium tumefaciens strain a311 carrying the plant tranfer vector pb1121, which contains the neomycin phosphotransferasell gene ( nptll ) and p - glucuronidase reporter gene ( gus ) both under the control of the camv 35s promoter, was used in the establishment of the genetic tranformation of white clover

    選用苗齡4 5天的帶柄子葉作為外植體，先將外植體預培2天，再與根癌農桿菌a311共培養3 4天後，轉入附加有40mg l ~ （ - 1 ）卡那黴素和400mg
  19. Optimization of a fermentation medium for linoleic acid isomerase production with lactobacillus acidophilus

    嗜酸乳桿菌發酵產亞油酸異構酶培養基條件的研究
  20. Thus the blue film of polydiacetylene and its mannose derivative were got and uv - vis 、 rrs 、 ftir 、 and cv were used to study the changes of these films when these films were incubated with e. colik12. the details of the article are as follows : the preparation of diacetylene and its mannose derivative monolayers : we successfully prepared the monolayers of diacetylene and its mannose derivative on the water surface by the ksv - 5000 trough

    本論文採用超分子組裝技術對雙炔及其甘露糖的衍生物進行了有序組裝，然後用紫外燈使其聚合，得到聚雙炔及其甘露糖的衍生物的單分子薄膜，並運用uv - vis 、 rrs 、 ftir 、 cv等實驗手段對該薄膜與大腸桿菌培養前後進行了詳細的研究。
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