條件突變株 的英文怎麼說
中文拼音 [tiáojiàntūbiànzhū]
條件突變株
英文
conditional mutant- 條 : Ⅰ名詞1 (細長的樹枝) twig 2 (條子) slip; strip 3 (分項目的) item; article 4 (層次; 秩序; 條...
- 件 : Ⅰ量詞(用於個體事物) piece; article; item Ⅱ名詞1. (指可以一一計算的事物) 2. (文件) letter; correspondence; paper; document
- 突 : Ⅰ動詞1 (猛沖) dash forward; shoot out 2 (高於周圍) protrude; bulgeⅡ副詞(突然) abruptly; sud...
- 株 : Ⅰ名詞1. (露在地面上樹木的根和莖) root and stem of a tree above the ground 2. (植株) individual plant; plant Ⅱ量詞(棵)
- 條件 : 1. (客觀的因素) condition; term; factor 2. (提出的要求) requirement; prerequisite; qualification
- 突變 : 1 (突然急劇的變化) sudden change; change suddenly; transilience; accident; saltation; revulsion...
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Antimicrobial agents susceptibility of this strain illustrated that it was resistant to gentamicin, streptomycin, ciprofloxacin, ceftazidime and imipinem
應用此條件經過不斷篩選,最後獲得對多種抗生素耐藥的突變菌株pa9911 - c64 。The xylanase activity of the mutant kept stable after 10 generations. after orthogonal designing experiment, the optimum fermentation conditions of a. niger j 506 were obtained, which is as followed : concentration of the major carbon resource 4 %, ratio between bran and corncob 5 : 5, concentration of glucose 0. 1 %, concentration of ammonium oxalate as supplemental nitrogen resource 2. 0 %, the initial ph of liquid medium 5. 0, 100ml / 250ml flask
經過正交試驗設計,得出突變株a . nigerj506產木聚糖酶最佳的工藝條件為:主碳源濃度4 、麩皮與玉米芯的比例為5 : 5 、輔加碳源葡萄糖的濃度是0 . 1 、輔加氮源草酸銨的濃度是2 . 0 ,培養基初始ph為5 . 0 , 250ml三角瓶的裝液量為100ml 。The future characterization and genetic analysis for candidate mutant were carried out and find that some candidate mutant ( such as roi30 doil - 1 doi0311131 ) have good phenotype by drought h2o2 aba - stressed treatment. at the same time we also observe the development of candidate mutant at different growth stages carefully. many modal difference between mutant an d wild type at the same period were found, such as more rosette layering fatty and big in leaves, advancment or delay for the flower period, rosettes living in the main stem, shorten in figure, the amount of seed little, sterilization etc. these physiological and modal changes may reflect with maladjustment in expressions of some gene and confusion on their inner control, . we will futher study concrete and detailed function mechanism
我們對這些擬南芥侯選突變體進行進一步的鑒定和遺傳學分析,發現ro口口、 doil 、 doi口jlll3i等潛在突變株對aba 、過氧化氫及早脅迫有明顯表型,同時對潛在突變體的生長發育進行了詳細的觀察,發現多數潛在突變株與同條件下野生型比出現了許多明顯的形態改變,如:蓮座基葉增多、分層、肥大,花期提前或延遲,主莖生輪座,株型矮化,產籽量少,不育,敗育等,這些生理和形態上的差異很可能反映了它們內部某些基因的表達受到了影響、代謝調控發生了紊亂,具體和詳細的作用機制還需要進一步的研究。Screening of high productive mutant of polyglutamic acid and its fermentation conditions
谷氨酸高產突變株的選育及搖瓶發酵條件. the conditions of the liquid state and solid state fermentation that m12 - 69 produced monaclin k were studied
第五,對紅麴黴突變菌株m12一69產monacofink的液態和固態發酵條件進行了初步優化研究。Using conjunction mobilization, we screened the mutant of eaggec 17 - 2 with the mutant phenotype by one step. compared mutant and eaggec 17 - 2, the experiments such as growth under iron - deficient conditions, virulence testing to the mouse, and ybt biosynthesis and regulation showed that ybtp, ybtq and ybtx genes have no significant effects on iron uptake, mouse virulen
Eaggec17毛攜帶的hpi毒力島的功能研究eaggec172的ybtp 、 ybtqyybtx突變株沒有表現出鐵缺陷培養條件下的生長抑製表型,對ybt的合成無明顯影響;對野生株與ybtp突變株的鼠毒力對比實驗表明, lds 。The liquid and solid fermentation conditions of m12 - 69 were also studied
對該突變株的液態和固態發酵條件進行初步優化研究。4. effect of resolution vector on the expression of pesticidal crystal protein genes this work successfully introduced pesticidal crystal protein genes into crystal negative strain bmb171 by using resolution shuttle vectors. after resolution, the expression of cry1ac and cry 1c genes have no obvious change, but the expression of cry3a genes has great increase in the same condition
解離反應對殺蟲晶體蛋白基因表達的影響成功地利用解離載體將crylac10 , crylc和cry3a基因導入蘇雲金芽胞桿菌無晶體突變株, crylac和crylc基因解離前後的表達量和殺蟲毒力未見明顯變化, cry3a基因在相同條件下則表達量有所提高,至於為何只對基因cry3a有作用尚不清楚,國內外也未見有人作相關報道。An auto - controlled fermentor was used to culture these strains under nitrogen fixation conditions and the nitrogneases were effectively depressed
在固氮條件下,所構建固氮酶突變菌株均表現為嚴格的nif ~ -表型,不能進行固氮生長。The mutant pel - d92l was expressed in pichia pastoris gs115, sds - page detection showed that the expression product pel - d92l - gs is different from pel - gs, and its " yield decreased dramatically, the themostability of pel - d92l - gs is also different from the pel - gs, but their optimum temperatures are same. 3. directed evolution of pel through random mutagenesis mutagenesis pcr carried out in error - prone conditions was used on the vector psk - pel, using the oligos " beginning " and " end ", homologous to the 5 ' and to the 3 " ends of the gene of pel respectively
三、 pel基因的隨機誘變用易錯pcr方法對pel基因進行隨機誘變, pcr產物與ppic3 . 5k連接,轉化大腸桿菌,獲得的混合質粒電轉化畢赤酵母gs115 , omm平板篩選適于低溫或對熱穩定的重組子,篩選獲得一株最活反應溫度、熱穩定性、發酵酶活均有提高的突變體pel - ep5 - gs ,其最活反應溫度為45 ,比野生型高出5 ; 40處理30min殘留活性為56 ,大大高於野生型的6 ;初始ph7 . 3528條件下培養72h ,發酵上清酶活為325u / ml 。分享友人