條帶組分 的英文怎麼說

中文拼音 [tiáodàizǔfēn]
條帶組分 英文
band constituent
  • 條 : Ⅰ名詞1 （細長的樹枝） twig 2 （條子） slip; strip 3 （分項目的） item; article 4 （層次; 秩序; 條...
  • 組 : Ⅰ名詞1 （由不多的人員組成的單位） group 2 （姓氏） a surname Ⅱ動詞（組織） organize; form Ⅲ量詞（...
  • 分 : 分Ⅰ名詞1. （成分） component 2. （職責和權利的限度） what is within one's duty or rights Ⅱ同 「份」Ⅲ動詞[書面語] （料想） judge
  1. The analysis of sds - page indicated a fusion protein band at the site of 20 - 30kda appeared in the form of inclusion body

    Sds - page分析表明，重組菌在分子量20 - 30kda處出現一高表達蛋白條帶，此誘導表達的蛋白在沉澱中以包涵體形式存在。
  2. The deleted mutant pap gene was also cloned into yeast secreted expression ppic9k vector to form ppic9k ~ 3, then the vector was transferred into pachia pastoris gs115 strain. the specific expression protein was secreted into the medium after inducing with methanol and the protein amount reached about 50 - 60 u g per millilitre measured by uv - absorbed methods in the supernatant of the medium via high density fermentation. sds - page results showed that there was one protein band in the gel which molecular weight was about 34ku

    將缺失型pap基因克隆于酵母分泌型表達載體ppicgk構成重組載體，然後導入畢赤酵母（ p8chianastoris ）菌株gslls細胞中，在甲醇的誘導下，經過酵母高密度發酵進行pap的表達，經sds page分析，結果表明，在培養基上清液中含有一明顯的特異性蛋臼條帶，大小為34ku ，經western blotting分析，該蛋白與法國pap抗血清有特異性反應，體外活性檢測表明該蛋白對tmv的侵染性具有高度的抑制性，說明該pap基因在畢赤酵母gs中也得到了正確表達。
  3. 300 clear bands including 289 polymorphic bands and 50 specific bands were amplified from the genomic dna of gastrodia elata bl. by these selected primers. the rate of polymorphic bands and the rate of specific bands were 98. 0 % and 16. 6 % respectively

    6個引物組合共擴增出300條清晰可辨的條帶，其中289條多態性條帶， 50條特異性條帶；多態性條帶率和特異性條帶率分別達98
  4. The fouling of monomer recovery system of qilu esbr plant was studied. it was con sidered that the fouling was principally caused b y the following four reasons, there existed the colopholic potassium containing c onjugated doublebonds in the disproportionated colopholic potassium, excessive co ntent of c16 - 18 compositions existed in the fatty acid, excessive content of com positions for which the demol condensation degree was above 5, the reaction conve rsion was higher than 72 percent. effective countermeasures were proposed

    介紹了齊魯引進乳聚丁苯橡膠裝置回收單元存在的設備堵掛問題，經分析認為歧化松香酸鉀中帶有含共軛雙鍵的松香酸鉀，脂肪酸中c16 18組分含量高， demol縮合度高於5的組分高，反應轉化率高於72 %是造成堵掛的4條主要原因，提出了對策。
  5. The ade + transformants were selected and fermented in flasks with 20ml bmmy medium, then, induced by 0. 5 % methanol. the expression protein was analyzed by sds - page after five days of induction. sds - page analysis revealed that the high - level expression recombinant strains of pmad16 / pmet a b / abp2304 and pmad16 / pmet a a / abp780 had specific bands at 75kd and 55kd separately, account for 30 % and 10 % of the total protein separately, which were purified using probond resin purification system, and obtained 15mg at levels above 0. 75g / l and 7mg expression protein at levels above 0. 35g / l separately once purification, the purity is both above 90 %

    篩選ade +表型轉化子， 20mlbmmy搖瓶培養，用0 . 5甲醇誘導表達5天後， sds - page檢測結果表明：選出的重組高效表達菌株pmad16 pmet b abp2304和pmad16 pmet a abp780都存在明顯的表達特異條帶，分子量分別為75kd和55kd ，分別占其總蛋白的30和10 ，經過probondresin鎳親和層析柱都得到了純化，其純度都在90以上，一次純化分別可得到大約15mg和7mg表達蛋白，推知表達量分別高達0 . 75g l和0 . 35g l以上。
  6. Through field investigation and analysis indoor, with studying all hydrochemistry data in detail, including macro components and micro components, the author finds out the hydrochemistry feature of ground water. for further specifying the ground water system, with cluster analysis of macro components of surface water and ground water in total 147 samples and the analysis of micro components, including ree, the main ground water systems are distinguished by and large, especially the ground water system main of fault no. 7 and fault no. 15 water bearing belts which have differences at macro and micro components between the two ground water systems, moreover, the main hydrogeochemistry effects are established such as lixiviation, oxidization, precipitation and mixing effect, especially the mixing effect which result in the complexit y of the hydrochemistry of deep bearing tectonic fracture water. based on them, the hydro geological model of upper dam base is established, meanwhile the author summarizes the hydrochemistry feature of weathering crevice water, surface tectonic crevice water and deep tectonic crevice water

    為此，本文以大崗山壩區水文地球化學問題為研究對象，通過野外調查和室內分析，詳細的研究了壩區水化學資料，包括宏量組分、微量組分，查明了壩區地下水水化學特徵，對採集的147個地表及地下水樣的宏量組分進行聚類分析，結合微量元素，稀土元素的研究，並應用二氧化硅地熱溫標確定了深部構造裂隙水的熱源深度，基本區分了壩區各個主要地下水水系，特別是以f7 、 f15斷裂含水帶為主的地下水系，它們的宏量組分、微量組分以及稀土等方面均存在差異，以此為基礎，結合壩區水文地質條件，建立了壩區的上壩址的水文地質模型，同時通過分析了壩區花崗巖區的水化學資料，確立了壩區主要的水文地球化學作用，分別為：溶濾作用、氧化作用、沉澱作用、以及混合作用，混合作用是導致深部承壓裂隙水水化學復雜的主要原因，並總結了壩區風化裂隙水、淺部構造裂隙水、深部構造裂隙水的水化學特徵。
  7. The results from sds - page presented that there were three female specific protein subunits with molecular weights of 123 kd, 120 kd and 91 kd, respectively. we can conclude the higher molecular compose of two subunits ; the results from two dimension electrophoresis showed the isoelectric points of two female - specific spots with molecular weight of about 120kd were 5. 5 and 5. 7. immunodiffusion reactions demonstrated that vg existed both in female fat body and hemolymph, which as vn was deposited in the ovary, while not in the male

    Page電泳結果表明：麗蠅蛹集金小蜂明顯存在2條雌特異性帶-卵黃蛋白，分子量分別為181kd和136kd ； sds - page電泳分析：存在3條雌特異性帶，其分子量為123kd 、 120kd和91kd ，由此，可推定卵黃原蛋白（ vitellogenin ， vg ）和卵黃磷蛋白（ vitellin ， vn ）由2個蛋白組成，其中分子量較大的蛋白由2個亞基組成；雙向電泳結果顯示，在120kd附近有兩個特異性點，其等電點為5 . 5和5 . 7 ；雙擴散表明，麗蠅蛹集金小蜂卵黃磷蛋白的抗血清與雌隱成蟲蟲體、脂肪體、血淋巴和卵巢勻漿液均有免疫沉澱反應，而與雄蜂血淋巴無免疫反應，說明了vg與vn具有免疫同源性，是雌特異性蛋白，且由脂肪體合成。
  8. Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid, and induced with methanol to secrete interesting peptide. the supernate of pichia pastoris culture was analysed by sds - page and western blotting. a reactive band, which the apparent molecular weight is 36kd, can be detected with sheep anti - hcmv polyclonal antibodies

    重組質粒轉化巴氏畢赤酵母， g418篩選出多拷貝插入的單克隆，甲醇誘導多拷貝插入的單克隆酵母細胞分泌目的蛋白，培養液上清經sds - page電泳分析，在蛋白質印跡中檢測到培養液上清有一表觀分子量為36kd ，能與羊抗hcmv多克隆抗體發生發應的條帶。
  9. When the approach to get a direct binding molecule was proved not fruitful, attention was turned to find binding sources for the purpose of further isolating the binding protein. the first considered and also the relatively easier - obtained materials are some adhesion molecules. results showed, only n - cam 140 showed ambiguous binding

    ， idin砰proteina磁珠為結合反應載體，從小鼠成年全腦勻漿作為結合起始材料，以蔗糖密度梯度離心法進4行細胞亞組分分離，以sds page顯示陽性結合條帶，以westernblot確定蛋白身份。
  10. Then, a piece of degenerate primer was designed according to the conserved amino acids of glycine betaine abc transporter system glycine betaine - binding protein as a reversed primer. combined with the opuaa - up, a 2. 3 kb fragment was obtained through pcr. blast result showed a fragment which contained the partial opuaa, the whole opuab and partial opuac sequences were obtained

    再次，根據甘氨酸甜菜堿atp轉運系統底物結合蛋白的氨基酸保守序列設計下游簡並引物，與atp結合蛋白的上游簡並引物組合，經pcr擴增獲得2 . 1kb的條帶，測序后通過blast比較，結果顯示獲得atp結合蛋白基因的部分序列、通透酶的全部編碼序列和部分甘氨酸甜菜堿結合蛋白基因的核苷酸序列。
  11. Sds - page showed efficient expression and secretion of the rhfl. the highest yield ( 108 mg / l ) was obtained when expression was induced with 0. 5 % methanol for 96 hours

    Western雜交實驗出現了可被抗體特異性識別的雜交帶，證實此條帶即為重組畢赤氏酵母km7lppicgkil分泌表達的重組fl蛋白。
  12. ( 2 ) effects on mouse spleen of so2 challenge : we found significant apoptotic changes of mouse spleen through tem observation and dna electrophoresis analysis and flow cytometric analysis. we found condensed, marginating, half - moon like apoptotic lymphocytes both in white pulp and red pulp ; we found significant dna degradation with dna ladders from the dna electrophoresis analysis in the 168 mg / m3 so2 treated group ; we also found marked increase of apoptotic rate between 168 mg / m3 so2 treated group and control group from the flow cytometric analysis

    （ 2 ）二氧化硫吸入可引起小鼠脾臟細胞出現明顯的凋亡改變，紅髓區和白髓區淋巴細胞出現核固縮，染色質凝聚、邊集； dna凝膠電泳分析發現168mg m ~ 3二氧化硫染毒組出現典型的dna梯形條帶；流式細胞分析也發現高劑量染毒組的小鼠脾細胞凋亡率增加，並且與對照組相比有顯著性差異， p 0 . 05 。
  13. In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee

    本實驗用pcr擴增hcv結構區基因，克隆到桿狀病毒表達載體pfastbacl中，構建成重組轉座載體pfb1 - cee ，轉化dh10bac大腸桿菌感受態細胞，篩選陽性菌落，抽提大分子質粒dna ，獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ，脂質體介導轉染sf9昆蟲細胞，出現細胞病變后，收集含有重組桿狀病毒顆粒的培養上消，重新感染sf9細胞，收集sf9細胞，進行12 . 5 sds -聚丙烯酰胺凝膠電泳，可見表達的蛋白條帶。
  14. The homologious comparison proved the cloned gene had 96 % homology to the sequence of the omp gene, and the alignment of the amino acid sequence was 98 %. the recombinant plasmid was constructed with the target gene and the expressing vector pgex - 4t - l and then was transformed into e. coli bl21 ( de3 ) the fusion protein was expressed under the iptg inducing condition, and exhibited about 62kda in size, very close to the predicted molecular weight of gst - momp. furthermore, the fusion protein was specifically recognized by anti - serum which raised against the major outer membrane protein of ahl316

    Sds - page電泳分析顯示誘導表達的基因產物分子量約為62kda ，與預測的gst -外膜蛋白重組融合蛋白的分子量極為相似， western - blot進一步證實，表達產物能被嗜水氣單胞菌l316主要外膜蛋白特異性抗血清所識別，產生明顯的染色條帶，說明所表達的基因產物與天然的外膜蛋白抗原性一致。
  15. Objective the origin and the nature of the hodgkin and reed - sternberg ( h / rs ) cell of hodgkin ' s lymphoma ( hl ) has been attracting a lot of medical researchers engaged in studying it, for its character by scattered large atypical cells residing in a complex admixture of inflammatory cells. great improvement has been made since a new method of isolation of single h / rs cells from a frozen section had been set up by kuppers in 1994, and many studies have approved of the b - cell derivation of h / rs cell. lt has been reported that h / r - s cells might partly be originated from b - cell in our research before, but at the same time, we also found that only 18. 8 % of h / rs cells express cd20, 31. 3 % of immunoglobulin heavy chain ( igh ) rearrangement have been revealed

    目的霍奇金淋巴瘤（ hodgkin ' slymphoma ， hl ）由於它的惡性腫瘤細胞? h rs細胞一般只佔腫瘤組織的極少部分（不到1 ） ，且散在分佈在背景細胞間，因此對于h rs細胞的來源和性質研究一直是人們探索的目標。 1994年德國科隆大學k ppers等發展了一種從冰凍組織切片上用顯微操作儀挑取單個h rs細胞的顯微切割方法進行h rs細胞基因分析后，人們對h rs細胞來源的研究有了突破性的進展，多數支持b細胞來源。我們的前期研究也支持部分h rs細胞為b細胞來源，但我們發現只有18 . 8的h rs細胞表達cd20 ， 31 . 3的hl檢測到igh基因重排克隆性條帶。
  16. The materials showed abundant polymorphism based on molecular level and the wild germplsms have less polymorphism than the cultivars. the number of polymorphic bands varies from 37 ( hekouyebajiao ) to 58 ( longxuan, huanong no 7, heijiaomang ), while the ratio of it is from 28. 03 to 43. 94 percent. 132 polymorphic distinct bands were obtained with two pairs of primers in this research, which is enough to distinguish the 60 materials from each other

    各種質之間的多態性帶在37 （河口野芭蕉）到58條（龍選，華農7號，黑腳芒）之間，多態性比例在28 . 03到43 . 94之間，選用e - acc m - cat和e - acc mcag兩對引物組合共擴增出132條dna條帶，足以把60個材料分開。
  17. Secondly, we compared and analyzed the growing state, plant height, flower shape and seed setting ability, etc, of m2 tube seedlings and m3 greenhouse plants treated with low - energy n *. we found that the growth rates of m2 tube seedlings and m3 greenhouse plants were lower than that of control and the growth rate decreased with the treated doses from 60x1015, 40x1015 to 80 x 1015. vfe also found that many phenotypic variations appeared in m2 tube seedlings, including yellowing, lethality, semi lethality, morphological variation ( such as leaf and flower shape variations, plant height variation, etc )

    最後，對80次劑量處現的擬南芥的mz代試管稍和60次劑員處理的m3代溫室茵採用rapd技術對dna的差異進行了分析，檢測發現經低能n 」注入處理的材料，其pcr條帶缺失或增加，而對照組未發現明顯的變化，而且條帶的變異率隨劑量的升高而上升，從引物的角度看， 80次劑量處理的比代試管苗的多態性表現為67
  18. The subunits of dpl were dpl - a 39. 6kd and dpl - 3 36. 8kd, respectively. it may consist two a subunits and two 3 subunits without disulphide bridges. dpl showed no specific agglutination with any type of human erythrocytes

    純化的dpl在page和等電聚焦中顯示一條帶，在非還原和還原sds - page中呈現兩條帶，分子量分別為- 39 . 6kd和- 36 . 8kd ，證明dpl由兩個亞基和兩個亞基組成，無二硫鍵。
  19. Meanwhile, the cloning and sequencing of which was also performed. and the main results were as follows : ( 1 ) only two obvious polymorphism bands were obtained in the fertile line from selective pcr amplification with 64 pairs e + 3 / m + rimer combinations, and which were named as mf - 14 and mf - 24, respectively. moreover the polymorphism band mf - 14 was cloned successfully

    結果如下： （ 1 ）本實驗分別以可育系和不育系基因組dna混合池為模板，選用64對e + 3 m3引物組合進行選擴篩選，分析得到了兩條可育系所特有的差異條帶mf - 14和mf - 24 。
  20. After a backup device is defined as part of a stripe set, it cannot be used for a single - device backup unless format is specified

    在備份設備已定義為條帶集的組成部分后，將不能用於單個設備備份，除非指定了format 。
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